Isolation and characterisation of the homogalacturonan from type II cell walls of the commelinoid monocot wheat using HF-solvolysis

Spatial Distribution of Selected Chemical Cell Wall Components in the Embryogenic Callus of Brachypodium distachyon

“…Pectins are also known to play protective roles in the interactions between plants and phytopathogens [52, 53]. To date, studies on the functions of pectins in monocots are very limited [34, 40, 54, 55]. In their study on androgenic cultures of wheat cultivars, Pilarska et al [56] demonstrated that the xylogalacturonan-targeting LM8 antibody gave signals only in a peripheral cell and that the JIM5 antibody specifically detected methyl ester-rich pectins, in the cell walls of the callus parenchyma, thus suggesting the importance of pectic substances in wheat androgenesis, and possibly in the regulation of cellular adhesion.…”

Advances in biomedical applications of pectin gels

“…To extract pectin from the middle lamella, cold and hot aqueous solutions of calcium chelating compounds, as ethylene diamine tetraacetate-EDTA [60], cyclohexane diamine tetraacetate -CDTA [61,62], imidazole buffer [45,62], sodium hexametaphosphate [40] or ammonium oxalate [63,64] remove calcium ions from calcium pectate without damaging the pectin galacturonic chains. However, the complete removal of the reagents is difficult to obtain upon purification, and the residual chelating agents may deeply affect the gelling properties of pectin, reducing the ability to form stable gels.…”

Pectic substances: From simple pectic polysaccharides to complex pectins—A new hypothetical model

“…Different kinds of HG cross-links are important for strengthening the cell wall, intercellular adhesion, and normal growth in vascular plants (O'Neill, Ishii, Albersheim, & Darvill, 2004;Sila et al, 2009). Various methods, including mild alkali (NaOH) hydrolysis (Link & Nedden, 1931;Morell, Baur, & Link, 1934), mild acid (HCl, H 2 SO 4 or HNO 3 ) hydrolysis (MAH) under different experimental conditions (Chambat & Joseleau, 1980;Jarvis, Forsyth, & Duncan, 1988;Thibault, Renard, Axelos, Roger, & Crépeau, 1993;Yapo, Robert, Etienne, Wathelet, & Paquot, 2007;Yapo, 2009a), hydrogen fluoride (HF)-solvolysis at −23 • C for at least 30 min (Mort, 1983;Wiethölter et al, 2003;Zhang, Pierce, & Mort, 2007) and enzymatic procedures combining rhamnogalacturonan-hydrolase (RG-ase), ␣-(1 → 5)-l-endo-arabinanase (Endo-A), ␤-(1 → 4)-dendo-galactanase (Endo-G), ␣-l-arabinofuranosidase (␣-Ara-ase), and ␤-d-galactosidase (␤-Gal-ase) (Hellín, Ralet, Bonnin, & Thibault, 2005;Nakamura, Furuta, Maeda, Takao, & Nagamatsu, 2002a;B. M. Yapo, unpublished results) have been reported to afford the isolation of HGs from extracted pectins or 'intact' CWMs.…”