Isolation and characterisation of proline/arginine-rich cathelicidin peptides from ovine neutrophils

ChBac3.4: A Novel Proline-Rich Antimicrobial Peptide from Goat Leukocytes

“…The results obtained by the broth microdilution assay allowed to compare the activity of ChBac5 with previously described bovine Bac5 and Bac7 (Gennaro et al 1989) and ovine Bac5 which were tested in similar conditions. The activity of ChBac3.4 against gramnegative bacteria closely matched to that of ChBac5 and that described for bovine Bac5, Bac7 and their N-terminal fragments (Gennaro et al 1989;Podda et al 2006;Benincasa et al 2004); and to the activity of Bac5, OaBac5mini and OaBac7.5mini of sheep (Anderson and Yu 2003;Anderson et al 2004). Conversely, ChBac3.4 was significantly more active against gram-positive bacteria than ChBac5 as well as than bovine Bac5, Bac7 and somewhat more active than a 24-residues N-terminal fragment of ovine Bac5 (OaBac5mini) and OaBac7.5mini, which also demonstrated some effects in respect with Staphylococci (Anderson et al 2004).…”

“…Pro-rich AMPs have been found in many invertebrate animals, including insects (Casteels et al 1989;Bulet et al 1993;Cociancich et al 1994), crustaceans (Destoumieux et al 1997;Schnapp et al 1996), and annelids . In vertebrate animals, prolinerich AMPs were discovered in cattle (Gennaro et al1989), sheep (Huttner et al 1998;Shamova et al 1999;Anderson et al 2003), goats , deer (Treffers et al 2005) and pigs (Agerberth et al 1991;Harwig et al 1995). Their high proline content and the presence of certain repeated proline motifs enables the peptides to interact with a variety of proteins that participate in signal transduction, transcription and other essential biological processes.…”

The influence of porcine cathelicidins on neutrophils isolated from rabbits in the course of bone graft implantation

“…Anderson and Yu (2003) showed that a crude extract obtained from ovine neutrophils had properties expanding beyond the properties displayed by proline/arginine rich peptides. This may be due to the presence of other ovine antimicrobial peptides which can act synergistically in the studied extract.…”

“…Apart from synthetic peptides, naturally obtained cathelicidins from neutrophils are considered for therapeutic usage (Anderson and Yu, 2003). Previously, the ovine neutrophil extract was assessed as the source of cathelicidins (Anderson and Yu, 2008).…”

Antimicrobial fragments of the pro-region of cathelicidins and other immune peptides

“…Our previous work showed that the majority of the antimicrobial activity of the ovine crude extract was due to a group of proline/arginine-rich cathelicidin peptides (Anderson and Yu 2003). Cathelicidins are synthesised in maturing myeloid cells in the bone marrow as pre-pro-peptides.…”