Isolation and characterisation of phytase from dormant Corylus avellana seeds

Andriotis, Vasilios M. E.; Ross, James D.

doi:10.1016/s0031-9422(03)00415-1

Cited by 19 publications

(29 citation statements)

References 36 publications

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“…These results were discussed as evidence of active metabolism in germinating seed [87]. In agreement, phytase is strongly and competitively inhibited by P i , while the decrease in phytase activity coincided with maximal IP6 turnover [88]. It was found that about 87% of IP6 is digested during the first 6 days of germination [89].…”

Section: Hydrolysis Of Phytic Acid During Seed Germinationmentioning

confidence: 69%

Metabolic Processes During Seed Germination

Ali¹,

Elozeiri²

2017

Advances in Seed Biology

125

102

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Seed germination is crucial stage in plant development and can be considered as a determinant for plant productivity. Physiological and biochemical changes followed by morphological changes during germination are strongly related to seedling survival rate and vegetative growth which consequently affect yield and quality. This study is aimed to focus on proceeding of the most vital metabolic processes namely reserve mobilization, phytohormonal regulation, glyoxylate cycle and respiration process under either stressful or non-stressful conditions that may be led to suggest and conduct the more successful experimental improvements. Seed imbibition triggered the activation of various metabolic processes such as synthesis of hydrolytic enzymes which resulted in hydrolysis of reserve food into simple available form for embryo uptake. Abiotic stresses potentially affect seed germination and seedling establishment through various factors, such as a reduction in water availability, changes in the mobilization of stored reserves, hormonal balance alteration and affecting the structural organization of proteins. Recent strategies for improving seed quality involved classical genetic, molecular biology and invigoration treatments known as priming treatments. H 2 O 2 accumulation and associated oxidative damages together with a decline in antioxidant mechanisms can be regarded as a source of stress that may suppress germination. Seed priming was aimed primarily to control seed hydration by lowering external water potential, or shortening the hydration period.

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Section: Hydrolysis Of Phytic Acid During Seed Germinationmentioning

confidence: 69%

Metabolic Processes During Seed Germination

Ali¹,

Elozeiri²

2017

Advances in Seed Biology

125

102

View full text Add to dashboard Cite

show abstract

“…Protein and Pi were quantified as in Andriotis and Ross (2003) with bovine serum albumin and KH 2 PO 4 as standards, respectively. Native-PAGE and native-IEF with post-electrophoresis staining for APase activity were performed as previously outlined (Andriotis and Ross 2003).…”

Section: Methodsmentioning

confidence: 99%

“…Native-PAGE and native-IEF with post-electrophoresis staining for APase activity were performed as previously outlined (Andriotis and Ross 2003).…”

Section: Methodsmentioning

confidence: 99%

“…During the purification of the three APase isozymes and for time-course experiments, all activity assays were performed at pH 5.6 as previously described (Andriotis and Ross 2003). However, the activity of each purified isozyme was assayed at the stated pH during subsequent biochemical characterisation (pH 3: APase1, pH 5.6: APase2, pH 6: APase3; all with 0.1 M citrate buffers).…”

Section: Apase Activity Assaymentioning

confidence: 99%

“…This is regarded as a limitation in our current understanding of the processes involved in the transition from the dormant to the germinating state. We have previously isolated and characterised the hazel acidic phytase (Andriotis and Ross 2003). In this paper we report on the APase complement of hazel seeds during dormancy relief.…”

Section: Introductionmentioning

confidence: 98%

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Isolation and partial characterisation of acid phosphatase isozymes from dormant oilseed of Corylus avellana L.

Andriotis

Ross

2004

Planta

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The acid phosphatase (orthophosphoric-monoester phosphohydrolase, EC 3.1.3.2) complement from dormant hazel (Corylus avellana L.) seeds was found to exhibit significant electrophoretic heterogeneity partially attributable to the presence of distinct molecular forms. In axiferous tissue, total acid phosphatase activity increased in a biphasic fashion during chilling, a treatment necessary to alleviate seed dormancy. Three acid phosphatase isozymes were isolated from cotyledons of dormant hazel seeds by successive ammonium sulphate precipitation, size-exclusion, Concanavalin A affinity, cation- and anion-exchange chromatographies resulting in 75-, 389- and 191-fold purification (APase1, APase2, APase3, respectively). The three glycosylated isoforms were isolated to catalytic homogeneity as determined by electrophoretic, kinetic and heat-inactivation studies. The native acid phosphatase complement of hazel seeds had an apparent Mr of 81.5 +/- 3.5 kDa as estimated by size-exclusion chromatography, while the determined pI values were 5.1 (APase1), 6.9 (APase2) and 7.3 (APase3). The optimum pH for p-nitrophenyl phosphate hydrolysis was pH 3 (APase1), pH 5.6 (APase2) and pH 6 (APase3). The hazel isozymes hydrolysed a variety of phosphorylated substrates in a non-specific manner, exhibiting low Km and the highest specificity constant (Vmax/ Km) for pyrophosphate. They were not primary phytases since they could not initiate phytic acid hydrolysis, while APase2 and APase3 had significant phospho-tyrosine phosphatase activity. Inorganic phosphate was a competitive inhibitor, while activity was significantly impaired in the presence of vanadate and fluoride.

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Purification and characterization of a novel acid phosphatase from the split gill mushroom Schizophyllum commune

Zhang

Chen

Jian

et al. 2013

J. Basic Microbiol.

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A monomeric acid phosphatase (ACP) with a molecular mass of 72.5 kDa was purified from fresh fruiting bodies of cultured Schizophyllum commune mushroom. The isolation procedure entailed ion exchange chromatography on DEAE-cellulose, CM-cellulose, and Q-sepharose, and gel filtration by fast protein liquid chromatography on Superdex 75. It demonstrated a unique N-terminal amino acid sequence of NAPWAQIDEV, which exhibited 60% amino acid identity to that of S. commune hypothetical histidine ACP based on its genome sequence, but less than 30% amino acid identity to that of other fungal ACPs previously reported. The ACP exhibited an optimum temperature at 50 °C, an optimum pH at pH 4.6, and was considerably stable at a pH range of 4.0 to 9.0, and a temperature range of 20-40 °C. The Km of the purified enzyme for ρ-nitrophenyl phosphate (ρNPP) was 0.248 mM and the Vmax was 9.093 × 10(-3) μM/min. ACP activity was strongly inhibited by Al(3+) and Fe(3+) , but enhanced by Co(2+) , Mg(2+) , and Ca(2+) at a concentration of 0.5 mM.

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Isolation and characterisation of phytase from dormant Corylus avellana seeds

Cited by 19 publications

References 36 publications

Metabolic Processes During Seed Germination

Metabolic Processes During Seed Germination

Isolation and partial characterisation of acid phosphatase isozymes from dormant oilseed of Corylus avellana L.

Purification and characterization of a novel acid phosphatase from the split gill mushroom Schizophyllum commune

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