Isolation and characterisation of mouse intestinal mesoangioblasts

Perin, Silvia; McCann, Conor J.; Coppi, Paolo De; Thapar, Nikhil

doi:10.1007/s00383-018-4373-7

Cited by 4 publications

(5 citation statements)

References 24 publications

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“…Thus, it allows for preserving the native cell phenotype, gene expression, and function ( 98 ). The disadvantage is represented by costs, complexity, and personnel specialization ( 99 , 100 ). LCM was used to isolate pericytes from snap-frozen human and mouse brains ( 101 ).…”

Section: Current Protocols For the Derivation Of Pericyte Populationsmentioning

confidence: 99%

Approaches for the isolation and long-term expansion of pericytes from human and animal tissues

Alvino

Mohammed

et al. 2023

Front. Cardiovasc. Med.

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Pericytes surround capillaries in every organ of the human body. They are also present around the vasa vasorum, the small blood vessels that supply the walls of larger arteries and veins. The clinical interest in pericytes is rapidly growing, with the recognition of their crucial roles in controlling vascular function and possible therapeutic applications in regenerative medicine. Nonetheless, discrepancies in methods used to define, isolate, and expand pericytes are common and may affect reproducibility. Separating pure pericyte preparations from the continuum of perivascular mesenchymal cells is challenging. Moreover, variations in functional behavior and antigenic phenotype in response to environmental stimuli make it difficult to formulate an unequivocal definition of bona fide pericytes. Very few attempts were made to develop pericytes as a clinical-grade product. Therefore, this review is devoted to appraising current methodologies’ pros and cons and proposing standardization and harmonization improvements. We highlight the importance of developing upgraded protocols to create therapeutic pericyte products according to the regulatory guidelines for clinical manufacturing. Finally, we describe how integrating RNA-seq techniques with single-cell spatial analysis, and functional assays may help realize the full potential of pericytes in health, disease, and tissue repair.

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Section: Current Protocols For the Derivation Of Pericyte Populationsmentioning

confidence: 99%

Approaches for the isolation and long-term expansion of pericytes from human and animal tissues

Alvino

Mohammed

et al. 2023

Front. Cardiovasc. Med.

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“…Hasta ahora, la cavidad peritoneal parece ser el entorno in vivo ideal para la incubación de TEI, y se ha objetivado la obtención de buenos pedículos vasculares al implantar el TEI en zonas como la membrana del cuerno uterino, el mesenterio intestinal y el omento (36). Con este mismo fin, se están desarrollando trabajos enfocados al estudio de los mesangioblastos como posible fuente de derivados vasculares (37), así como el factor de crecimiento de fibroblastos básico (bFGF) que, a través de una liberación mantenida, ha demostrado en trabajos experimentales favorecer la angiogénesis así como estimular la proliferación de muchos componentes del tejido intestinal esenciales en su funcionamiento, como lo son las células del músculo liso (38). Asimismo los mesangioblastos contribuyen a la regeneración de la pared neuromuscular que, junto con las células de músculo liso intestinal constituyen un componente esencial para la restauración funcional de la contractilidad de cualquier órgano desarrollado mediante ingeniería tisular (37).…”

Section: Cirugía Generalunclassified

“…Con este mismo fin, se están desarrollando trabajos enfocados al estudio de los mesangioblastos como posible fuente de derivados vasculares (37), así como el factor de crecimiento de fibroblastos básico (bFGF) que, a través de una liberación mantenida, ha demostrado en trabajos experimentales favorecer la angiogénesis así como estimular la proliferación de muchos componentes del tejido intestinal esenciales en su funcionamiento, como lo son las células del músculo liso (38). Asimismo los mesangioblastos contribuyen a la regeneración de la pared neuromuscular que, junto con las células de músculo liso intestinal constituyen un componente esencial para la restauración funcional de la contractilidad de cualquier órgano desarrollado mediante ingeniería tisular (37). Además de los miocitos, es necesario un plexo nervioso plenamente funcional que favorezca una motilidad efectiva, para lo cual las células madre pluripotentes inducidas (iPSC) y las células derivadas de la cresta neural están mostrando resultados prometedores (39).…”

Section: Cirugía Generalunclassified

Application of tissue engineering in paediatric

Botía Martínez,

Fernández Valadés,

Alaminos Mingorance

2023

Actual. Med.

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The aim of this work is to review the current state of the use of tissue engineering in the field of pediatric surgery. We conducted a search of articles in PubMed under the terms of “Pediatric Surgery AND Tissue Engineering”. All those articles that included in the name of the journal and / or of the article any of the following terms were selected: “bioengineered”, “engineering”, “pediatric”, “children” “pediatric surgery”; In addition to those articles resulting from this same search, which, in our opinion, should be included in our review because they also deal with issues related to tissue engineering applied to disciplines that are part of the daily healthcare task of a pediatric surgeon. 178 articles were obtained. After carrying out a first review, we eliminated those referring to the applications of tissue engineering in cardiovascular surgery, traumatology, dentistry, and otorhinolaryngology, as they are specialties not included within pediatric surgery in our setting. Finally, 117 articles distributed as follows were reviewed: 36 on the area of general surgery (6 bibliographic reviews, 30 experimental), 26 plastic surgery (3 clinical trials, 15 experimental, 8 bibliographic reviews), 33 urology (26 experimental, 7 bibliographic reviews), 22 cleft palate (1 clinical trial, 13 experimental, 8 bibliographic reviews). There are many works related to tissue engineering and its application to pediatric surgery and, although in the theoretical panorama tissue engineering promises to be a hopeful therapeutic alternative in many pathologies included within this specialty, and although there are multiple experimental studies that suggest a hopeful application in the clinic, there are few works that deal with its real application in pediatric patients.

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“…Furthermore, studies on muscle cell culture, including the use of mesoangioblasts to generate skeletal muscle in engineered oesophagus 20 and the first report of isolation and characterisation of mesoangioblasts from small intestinal tissue 135 , offer insights into the continued improvements of smooth muscle cells for ITE.…”

Section: Engineering Muscle Enteric Nerves and Vasculaturementioning

confidence: 99%

Building gut from scratch — progress and update of intestinal tissue engineering

Tullie

Jones

Coppi

et al. 2022

Nat Rev Gastroenterol Hepatol

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Short bowel syndrome (SBS), a condition defined by insufficient absorptive intestinal epithelium, is a rare disease, with an estimated prevalence up to 0.4 in 10,000 people. However, it has substantial morbidity and mortality for affected patients. The mainstay of treatment in SBS is supportive, in the form of intravenous parenteral nutrition, with the aim of achieving intestinal autonomy. The lack of a definitive curative therapy has led to attempts to harness innate developmental and regenerative mechanisms to engineer neo-intestine as an alternative approach to address this unmet clinical need. Exciting advances have been made in the field of intestinal tissue engineering (ITE) over the past decade, making a review in this field timely. In this Review, we discuss the latest advances in the components required to engineer intestinal grafts and summarise the progress of ITE. We also explore some key factors to consider and challenges to overcome when transitioning tissue engineered intestine towards clinical translation and provide the future outlook of ITE in therapeutic applications and beyond. BlurbIntestinal tissue engineering offers a potential therapeutic option for short bowel syndrome. This Review examines the progress in intestinal tissue engineering, discusses the components required for engineered intestinal grafts, preclinical progress and efforts towards clinical translation, including challenges to overcome. Key points• Intestinal tissue engineering has the potential to offer curative therapy for patients with short bowel syndrome • Multiple components, including an absorptive mucosa, smooth muscle, enteric nerves and vasculature are required to generate a functional full thickness intestinal graft• Advances in intestinal tissue engineering include endothelial cell reprogramming and vascular engineering, generation of mucosal grafts using patient-derived materials and colon mucosal repurposing using small intestinal organoids.• Vascularisation and lymphatic engineering, generation of multi-layered personalised intestinal grafts and scaling-up of graft size present some of the future challenges in

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Isolation and characterisation of mouse intestinal mesoangioblasts

Cited by 4 publications

References 24 publications

Approaches for the isolation and long-term expansion of pericytes from human and animal tissues

Approaches for the isolation and long-term expansion of pericytes from human and animal tissues

Application of tissue engineering in paediatric

Building gut from scratch — progress and update of intestinal tissue engineering

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