Isolation and characterisation of microsatellite loci in the tire track eel, Mastacembelus favus and cross-species amplification

Genetic diversity of the tire track eel Mastacembelus favus in Southeast Asia inferred from microsatellite markers

“…Samples from caudal fin were clipped and immediately preserved in 90% ethanol until DNA extraction was performed. Total Genomic (Jamsari et al 2014) were used in a multiplex genotyping panel. Hale et al (2012) suggested that estimates of H E based on loci with low polymorphism are likely to be less accurate than those based on highly polymorphic loci, even when reasonably large samples are taken from each population.…”

“…Therefore, we only selected loci with high polymorphism to increase the accuracy and precision of expected heterozygosities. Seven selected loci were shown to be highly informative with high polymorphism (PIC > 0.5) and only one locus was moderately informative with moderate polymorphism (0.25 < PIC < 0.5) (Jamsari et al 2014). Each primer pair was fluorescently labeled at the 5 ' end (FAM or HEX) of the forward primer.…”

“…The PCR protocol consisted of an initial 95°C for 5 min followed by 35 cycles of 95 °C for 30 s, 50 °C for 30 s, 72 °C for 30 s and a final 10 min extension at 72°C. The PCR products were then pooled in two 25 µL mixes (Jamsari et al 2014) and genotyped by First BASE Laboratories Sdn. Bhd.…”

“…The aim of the study was to assess genetic diversity and population genetic structure of M. favus across its geographical distribution based on eight microsatellite markers developed by Jamsari et al (2014). Analysis of these genetic data will provide useful information for appropriate conservation and management plan of this fish species.…”