Isolation and characterisation of endophytic actinobacteria and their effect on the growth and nodulation of chickpea (Cicer arietinum)

Vo, Q. A. Tuan; Ballard, Ross; Barnett, Stephen J.; Franco, Christopher M. M.

doi:10.1007/s11104-021-05008-6

Cited by 13 publications

(9 citation statements)

References 61 publications

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“…CP84B, Streptomyces spp. CP200B and CP21A were previously isolated from chickpea as endophytes and showed significant effects on chickpea growth and nodulation (Vo et al 2021). These endophytic actinobacteria were grown on Mannitol Soy flour agar (MS) and incubated at 27 °C for 10-14 days until spores matured.…”

Section: Experimental Designmentioning

confidence: 99%

“…CP84B, Streptomyces spp. CP200B and CP21A, increased chickpea growth and nodulation (Vo et al 2021), the underlying mechanisms responsible for the improvements are examined in this paper. Understanding the modes of action is critical to improve their efficacy, and to assist future endophyte selection and optimization of their application in agriculture.…”

Section: Introductionmentioning

confidence: 99%

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Revealing the underlying mechanisms mediated by endophytic actinobacteria to enhance the rhizobia - chickpea (Cicer arietinum L.) symbiosis

Barnett

et al. 2022

Plant Soil

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Purpose The effects of endophytic actinobacterial strains, Microbispora sp. CP56, Actinomadura sp. CP84B, Streptomyces spp. CP200B and CP21A, on the chickpea-Mesorhizobium symbiosis, were investigated in planta, with the aim of revealing the underlying mechanisms of action. Methods The actinobacterial endophytes were co-inoculated with Mesorhizobium ciceri onto chickpea seedlings to study the effect on plant growth parameters, nodulation development and grain yield. The role of actinobacterial exudates on rhizobial growth was investigated, as was the role of root exudates of actinobacteria-colonized plants on the expression of rhizobial nod factors and biofilm formation. Changes in expression of plant flavonoids and bacterial N-fixation genes resulting from actinobacterial co-inoculation were assessed using qPCR. Results Application of actinobacterial endophytes, together with M. ciceri, showed growth promotion of chickpea with an increase in root nodule number and weight. Enhanced nodulation was accompanied by increases in total plant nitrogen, larger total plant weight and a 2–3-fold increase in grain yield. Factors associated with this tripartite symbiosis are promotion of rhizobial growth, earlier nodule formation, increased secondary root formation, up-regulated expression of genes related to flavonoid synthesis and nif genes. In addition, exudates of chickpea roots colonised with actinobacteria increased nodulation-related biological processes - rhizobial chemotaxis, biofilm formation and nod gene expression. Conclusion These endophytic actinobacteria positively affect many aspects of the chickpea-Mesorhizobium symbiosis resulting in increases in grain yield. Similar improvements recorded in chickpea growing in potted field soils, shows the potential to enhance chickpea production in the field.

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Section: Experimental Designmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Revealing the underlying mechanisms mediated by endophytic actinobacteria to enhance the rhizobia - chickpea (Cicer arietinum L.) symbiosis

Barnett

et al. 2022

Plant Soil

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“…Besides, there were other genera such as Actinomadura, Microbiospora, Micromonospora and Nocardia also shared in two or three C. oleifera samples. Studies have reported that these actinobacterial genera showed potential for use as biofertilizers in other crops including chickpea [56], alfalfa [57], Casuarina glauca [58]. These suggested that the endophytic actinobacteria from C. oleifera possessed wide range of application and great development value.…”

Section: Discussionmentioning

confidence: 99%

Biodiversity of Culturable Endophytic Actinobacteria Isolated from High Yield Camellia oleifera and Their Plant Growth Promotion Potential

Cui

Chen

et al. 2021

Agriculture

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Camellia oleifera Abel. is one of the world’s four famous woody oil trees and has drawn increasing attention because of its high commercial value. Endophytes are microorganisms inhabiting inside plant tissues, and their functions vary with the change of host status and environmental parameters. To deepen our understanding of the interactions between C. oleifera and their endophytic actinobacteria, the present study investigated the four endophytic actinobacterial composition-residing high-yield C. oleifera trees. A total of 156 endophytic actinobacterial isolates were obtained distributed in 17 genera. Among them, Streptomyces was the dominant endophytic actinobacteria, followed by Nocardia, Amycolatopsis, Microbiospora, Micromonospora and other rare actinobacteria genera. Soil characteristics including soil pH and organic matter were found to play crucial roles in shaping the endophytic actinobacterial community composition. Furthermore, all isolates were studied to determine their plant growth-promotion traits, 86.54% could produce Indole 3-Acetic Acid, 16.03% showed nitrogen-fixing, 21.15% showed phosphorus solubilizing, and 35.26% produced siderophore. Under the glasshouse condition, some isolates exhibited growth promotion effects on C. oleifera seedlings with significant increase in spring shoot length and ground diameter. Altogether, this study demonstrated that C. oleifera plants harbored a high diversity and novelty of culturable endophytic actinobacteria, which represent important potential as natural biofertilizers for the high production of C. oleifera.

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“…In fact, a wide diversity of strains belonging to this genus has been isolated from Medicago nodules in different countries (Australia, Spain) and different soils (Martínez-Hidalgo et al 2020). Beneficial endophytic Streptomyces have been isolated from plant roots and leguminous nodules (Coombs and Franco 2003;Le et al 2016;Mukhtar et al 2020;Vo et al 2021), and as shown in this study, two Streptomyces strains were also isolated from Medicago root nodules. Streptomyces (isolated from nodules and roots) and Mesorhizobium ciceri coinoculation increased the dry weight of chickpea (Vo et al 2021) supporting the hypothesis that nodule isolates are effective inocula.…”

Section: Discussionmentioning

confidence: 53%

“…Beneficial endophytic Streptomyces have been isolated from plant roots and leguminous nodules (Coombs and Franco 2003;Le et al 2016;Mukhtar et al 2020;Vo et al 2021), and as shown in this study, two Streptomyces strains were also isolated from Medicago root nodules. Streptomyces (isolated from nodules and roots) and Mesorhizobium ciceri coinoculation increased the dry weight of chickpea (Vo et al 2021) supporting the hypothesis that nodule isolates are effective inocula. In summary, a large number of diverse Gram-positive and Gram-negative bacteria belonging to 10 different genera were obtained.…”

Section: Discussionmentioning

confidence: 67%

Medicago root nodule microbiomes: insights into a complex ecosystem with potential candidates for plant growth promotion

et al. 2021

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microbes within and upon them. We analyzed the microbiomes of these underground organs from both an important crop plant (Medicago sativa) and a related legume (M. polymorpha) using metagenomic and culture-based techniques to identify the main cultivatable contributors to plant growth enhancement. Methods Using high-throughput sequencing, culturing, and in planta techniques, we identified and analyzed a broad population of the bacterial taxa within Medicago nodules and the surrounding soil. Results Fifty-one distinct bacterial strains were isolated and characterized from nodules of both Medicago

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Isolation and characterisation of endophytic actinobacteria and their effect on the growth and nodulation of chickpea (Cicer arietinum)

Cited by 13 publications

References 61 publications

Revealing the underlying mechanisms mediated by endophytic actinobacteria to enhance the rhizobia - chickpea (Cicer arietinum L.) symbiosis

Revealing the underlying mechanisms mediated by endophytic actinobacteria to enhance the rhizobia - chickpea (Cicer arietinum L.) symbiosis

Biodiversity of Culturable Endophytic Actinobacteria Isolated from High Yield Camellia oleifera and Their Plant Growth Promotion Potential

Medicago root nodule microbiomes: insights into a complex ecosystem with potential candidates for plant growth promotion

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