Isolation and characterisation of carbapenem-resistant Pseudomonas aeruginosa from hospital environments in tertiary care hospitals in Dhaka, Bangladesh

Saha, Kuntal K.; Kabir, Nayel D.; Islam, Md. Rayhanul; Amin, Mohammed Badrul; Hoque, Kazi Injamamul; Halder, Kakali; Saleh, Ahmed Abu; Parvez, Md. Masud; Begum, Khurshida; Alam, Md. Akhtarul; Islam, Mohammad Aminul

doi:10.1016/j.jgar.2022.04.008

Cited by 14 publications

(17 citation statements)

References 47 publications

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“…Previous studies also suggested that larger number of patients may harbor frequent source of drug resistant bacterial infections and prolonged hospitalization may increase the risk of acquisition of drug resistance elements through horizontal gene transfer (38, 39). A recent study in Bangladesh also demonstrated that hospital environment is a major reservoir for carbapenem-resistant P. aeruginosa with MBL carriage (40).…”

Section: Discussionmentioning

confidence: 99%

Metallo-β-lactamase mediated rapid increase in carbapenem resistance ofPseudomonas aeruginosa

Anjum,

Mitu,

Arefin

et al. 2024

Preprint

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Antibiotic-resistantPseudomonas aeruginosais a common nosocomial pathogen all over the world. We detected the presence ofP. aeruginosain 22% (53 out of 238) of the test samples collected from patients with infections including secondary wound infections, abscesses and urinary tract infections admitted to two academic hospitals in Bangladesh. Resistance to carbapenems (imipenem, and meropenem) was present among 30% (16 out of 53) of these clinical P. aeruginosa isolates, which is more than 2-fold higher compared to that of previous studies. Such a rapid increase in carbapenem resistance was mediated by metallo-β-lactamase (MBL). Expression of MBL was detected in 90% (14 out of 16) of these resistant isolates. Molecular analyses revealed that the carbapenem-resistant isolates carried at least one of the MBL variants, either bla-VIM or bla-NDM-1. All the bla-NDM-1 positives carried a 0.5 MDa plasmid. ERIC-PCR revealed the highly heterogeneous nature of theP. aeruginosaisolates indicating multiple sources of infection within the hospital. However, the majority of XDR isolates belonged to a single cluster of drug-resistant bacterial infections. These findings indicate that Metallo-β-lactamase (MBL) mediated resistance to carbapenem inP. aeruginosaposes a serious threat to the spread of infections among hospitalized patients.

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Section: Discussionmentioning

confidence: 99%

Metallo-β-lactamase mediated rapid increase in carbapenem resistance ofPseudomonas aeruginosa

Anjum,

Mitu,

Arefin

et al. 2024

Preprint

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“…Previous studies in Bangladesh have focused on clinical isolates, healthcare workers’ mobile phones, and even the genomic diversity of MDR P . aeruginosa from burn patients [ 4 , 13 , 38 ]. Therefore, this study was designed to determine the antibiotic resistance profiles and virulence genes distribution in P .…”

Section: Discussionmentioning

confidence: 99%

Characterization of β-lactamase and virulence genes in Pseudomonas aeruginosa isolated from clinical, environmental and poultry sources in Bangladesh

Islam,

Ferdous,

Hoque

et al. 2024

PLoS ONE

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The emergence and spread of multidrug-resistant pathogens like Pseudomonas aeruginosa are major concerns for public health worldwide. This study aimed to assess the prevalence of P. aeruginosa in clinical, environmental, and poultry sources in Bangladesh, along with their antibiotic susceptibility and the profiling of β-lactamase and virulence genes using standard molecular and microbiology techniques. We collected 110 samples from five different locations, viz., BAU residential area (BAURA; n = 15), BAU Healthcare Center (BAUHCC; n = 20), BAU Veterinary Teaching Hospital (BAUVTH; n = 22), Poultry Market (PM; n = 30) and Mymensingh Medical College Hospital (MCCH; n = 23). After overnight enrichment in nutrient broth, 89 probable Pseudomonas isolates (80.90%) were screened through selective culture, gram-staining and biochemical tests. Using genus- and species-specific PCR, we confirmed 22 isolates (20.0%) as P. aeruginosa from these samples. Antibiogram profiling revealed that 100.0% P. aeruginosa isolates (n = 22) were multidrug-resistant isolates, showing resistance against Doripenem, Penicillin, Ceftazidime, Cefepime, and Imipenem. Furthermore, resistance to aztreonam was observed in 95.45% isolates. However, P. aeruginosa isolates showed a varying degree of sensitivity against Amikacin, Gentamicin, and Ciprofloxacin. The blaTEM gene was detected in 86.0% isolates, while blaCMY, blaSHV and blaOXA, were detected in 27.0%, 18.0% and 5.0% of the P. aeruginosa isolates, respectively. The algD gene was detected in 32.0% isolates, whereas lasB and exoA genes were identified in 9.0% and 5.0% P. aeruginosa isolates. However, none of the P. aeruginosa isolates harbored exoS gene. Hence, this study provides valuable and novel insights on the resistance and virulence of circulating P. aeruginosa within the clinical, environmental, and poultry environments of Bangladesh. These findings are crucial for understanding the emergence of β-lactamase resistance in P. aeruginosa, highlighting its usefulness in the treatment and control of P. aeruginosa infections in both human and animal populations.

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“…However, sufficient information is unavailable on the environmental distribution of potential pathogenic MDR P. aeruginosa in Bangladesh. Previous studies in Bangladesh have focused on clinical isolates, healthcare workers’ mobile phones, and even the genomic diversity of MDR P. aeruginosa from burn patients [4, 36, 37]. Therefore, this study was designed to determine the antibiotic resistance profiles and virulence genes distribution in P. aeruginosa circulating in different environments of Bangladesh.…”

Section: Discussionmentioning

confidence: 99%

“…Their presence underscores the potential for these bacteria to cause severe infections and emphasizes the need for stringent infection control measures and judicious use of antibiotics to mitigate the emergence and spread of multidrug-resistant strains The difference between the results of this study and other investigations might be attributed to differences in various circumstances comprising incubators or to commonly occurring hyper-mutation among P. aeruginosa strains exhibiting varied antibiotic resistance. Furthermore, antibiotic-resistant bacteria can swiftly spread across the food chain and cause the majority of public health problems [34, 36].…”

Section: Discussionmentioning

confidence: 99%

Characterization of β-lactamase and virulence genes inPseudomonas aeruginosaisolated from clinical, environmental and poultry sources in Bangladesh

Islam,

Ferdous,

Hoque

et al. 2023

Preprint

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The emergence and spread of multidrug-resistant pathogens likePseudomonas aeruginosaare major concerns for public health worldwide. This study aimed to investigate the prevalence of circulatingP. aeruginosaisolated from clinical, environmental and poultry sources in Bangladesh, their antibiotic susceptibility, β-lactamase and virulence gene profiling using standard molecular and microbiology techniques. We collected 110 samples from five different locations,viz., BAU residential area (BAURA; n=15), BAU Healthcare Center (BAUHCC; n = 20), BAU Veterinary Teaching Hospital (BAUVTH; n=22), Poultry Market (PM; n=30) and Mymensingh Medical College Hospital (MCCH; n=23). After overnight enrichment in nutrient broth, 89 probablePseudomonasisolates (80.90%) were screened through selective culture, gram-staining and biochemical tests. Using genus- and species-specific PCR, we confirmed 22 isolates (20.0%) asP. aeruginosafrom these samples. Antibiogram profiling revealed that 100.0%P. aeruginosaisolates (n = 22) were multidrug-resistant isolates, showing resistance against Doripenem, Penicillin, Ceftazidime, Cefepime, and Imipenem. Furthermore, resistance to aztreonam was observed in 95.45% isolates. However,P. aeruginosaisolates showed a varying degree of sensitivity against Amikacin, Gentamicin, and Ciprofloxacin. TheblaTEMgene was detected in 86.0% isolates, whileblaCMY,blaSHVandblaOXA,were detected in 27.0%, 18.0% and 5.0% of theP. aeruginosaisolates, respectively. ThealgDgene was detected in 32.0% isolates, whereaslasBandexoAgenes were identified in 9.0% and 5.0%P. aeruginosaisolates. However, none of theP. aeruginosaisolates harboredexoSgene. Thus, this study provides novel and important data on the resistance and virulence ofP. aeruginosacurrently circulating in clinical, environmental and poultry environment of Bangladesh. These data provide important insights into the emergence of β-lactamase resistance inP. aeruginosa, highlighting its usefulness in the treatment and control ofP. aeruginosainfections in both humans and animals.

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Isolation and characterisation of carbapenem-resistant Pseudomonas aeruginosa from hospital environments in tertiary care hospitals in Dhaka, Bangladesh

Cited by 14 publications

References 47 publications

Metallo-β-lactamase mediated rapid increase in carbapenem resistance ofPseudomonas aeruginosa

Metallo-β-lactamase mediated rapid increase in carbapenem resistance ofPseudomonas aeruginosa

Characterization of β-lactamase and virulence genes in Pseudomonas aeruginosa isolated from clinical, environmental and poultry sources in Bangladesh

Characterization of β-lactamase and virulence genes inPseudomonas aeruginosaisolated from clinical, environmental and poultry sources in Bangladesh

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