Isolation and Characterisation of Biosurfactant-producing Pseudomonas specie from Soil

Kabir, Kamaludeen

doi:10.47430/ujmr.1942.001

Cited by 2 publications

(2 citation statements)

References 31 publications

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“…The mixture was then shaken for 24 hours at 180 rpm using an orbital shaker and 0.1mL of the sample was poured on a freshly prepared nutrient agar. The colonies were observed, counted and recorded as colony forming unit per gram of soil (CFU/g) after 24 hours (Aminu et al, 2022;Kabir, 2019).…”

Section: Isolation Of Heterotrophic Bacteriamentioning

confidence: 99%

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Identification and Screening of Biosurfactant Producing Bacteria from Mechanic Workshops Soil in Gusau Metropolis, Nigeria

Aliyu,

Usman,

Mohammed

et al. 2023

USci

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Biosurfactants are molecules that reduce interfacial tension. Their chemical composition can vary widely, but they have in common their amphiphilic or amphipatic nature and can thus be soluble in aqueous as well as in organic solvents. The study was carried out to identify and screen biosurfactant producing bacteria from mechanic workshops in Gusau metropolis. Eight (8) soil samples were collected at the depth of 0-7 and 8-15cm from selected mechanic workshops located at Gada Biyu, Taqama bye-pass, Birnin Ruwa and non-oil contaminated soil as control for analysis. The physicochemical parameters were analysed using standard procedures (blood heamolysis, drop collapse, oil displacement and emulsification index) methods were used to screen biosurfactant production by the isolates. Mineral salt medium supplemented with 1% Actual gasoline oil (AGO) as sole sources of carbon was used to isolate hydrocarbon degrading bacteria, while heterotrophic bacteria were isolated using nutrient Agar. The soil samples from the study area were characterised with smooth, grey to dark brown soil with an unpleasant smell as well as as well as high temperature and pH. The three mechanic workshops used for this study, shows relatively higher counts for Hydrocarbon Degrading Bacteria while higher heterotrophic bacterial count was obtained from the control site though there was no significant difference between individual mechanic workshop (p>0.05). The isolates are potential biosurfactant producers based on their performance especially blood haemolysis and emulsification index. The isolates identified belonged to the species of Bacillus, Pseudomonas, Acinetobacter, Micrococcus and Serratia.

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Section: Isolation Of Heterotrophic Bacteriamentioning

confidence: 99%

“…Blood agar plates were prepared and the newly formed single colonies from the isolated cultures were streaked and incubate at 37 °C, for 24 hrs as described by Kabir (2019) and Kurniati et al,. (2019), the plates were then observed for a clear zone around the colonies.…”

Section: Haemolytic Activitymentioning

confidence: 99%

Identification and Screening of Biosurfactant Producing Bacteria from Mechanic Workshops Soil in Gusau Metropolis, Nigeria

Aliyu,

Usman,

Mohammed

et al. 2023

USci

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Biodegradation of Premium Motor Spirit using surfactant-expressing bacteria from mechanic workshops in Malumfashi, Katsina State, Nigeria

Aliyu¹,

Onusiriuka²,

Umar³

et al. 2022

UJMR

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This study was aimed at investigating the potential use of biosurfactant-expressing bacterial isolates in the biodegradation of premium motor spirit (PMS) at various concentrations (100-100,000ppm). The biosurfactant-expressing bacteria were isolated from mechanic workshop in Malumfashi, Katsina, Nigeria using standard techniques. The isolates identified belonged to the genera Acinetobacter, Bacillus, Micrococcus, Pseudomonas and Stenotrophomonas. These isolates were screened for biosurfactant expression using drop collapse, haemolysis, oil–water behavior assays and emulsification index test. Positives isolates were investigated for PMS degradation by growing the isolates on mineral salt media supplemented with (0.1ml) premium motor spirit (PMS) as sole source of carbon. Although, higher total hydrocarbon degrading bacterial counts were obtained from soils where isolates positive for biosurfactant expression are predominant, there was no statistically significant difference between isolate source using Kruskal-Wallis H test (p = 0.67). The isolates Bacillus velezensis and Stenotrophomonas maltophilia were positive for biosurfactant-production potential using drop-collapse, β-haemolysis, oil spreading, and emulsification index and drop collapse tests with higher tolerance to PMS at concentrations up to 100,000 ppm. Statistical analysis using multiple-comparison analysis of variance (ANOVA) confirmed that the isolates exhibited varying PMS degradation response (p = 0.0066); furthermore, the tolerance of the bacteria to the PMS is dose-dependent (p = 0.00012). Post-hoc analysis using Tukey’s test identified Bacillus velezensis as the most efficient biosurfactant-producing and hydrocarbon degrading isolate (p = 0.0264 and 0.0034); moreover, the threshold concentration for high PMS tolerance was found to be 1000ppm and above (p = 0.0174, 0.0008 and 0.0001).These isolates’ ability to grow on mineral salt media supplemented with PMS as a sole source of carbon presents a veritable avenue for exploitation in biotechnology, towards biosurfactants-mediated bioremediation of hydrocarbon pollutants in oil contaminated soils. Keywords: Biodegradation, Petroleum Hydrocarbons, Premium Motor Spirit

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Isolation and Characterisation of Biosurfactant-producing Pseudomonas specie from Soil

Cited by 2 publications

References 31 publications

Identification and Screening of Biosurfactant Producing Bacteria from Mechanic Workshops Soil in Gusau Metropolis, Nigeria

Identification and Screening of Biosurfactant Producing Bacteria from Mechanic Workshops Soil in Gusau Metropolis, Nigeria

Biodegradation of Premium Motor Spirit using surfactant-expressing bacteria from mechanic workshops in Malumfashi, Katsina State, Nigeria

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