Isolation and Characterisation of Bergenin From Ethyl Acetate Extract of Flueggea Virosa Leaves

Abstract

Bergenin is an important constituent of Flueggea virosa (Euphorbiaceae), a tropical plant with several traditional uses. While there are numerous reports on the isolation and characterization of bergenin, a rapid, high-throughput, readily accessible method for the isolation and characterization of the compound locally has not been reported. Isocratic elution of ethyl acetate extract via vacuum liquid chromatography (VLC) with methanol produced a white amorphous solid (100 mg), which was successfully isolated from 250 g of the plant. On the basis of spectral data (1H, C NMR, COSY, HSQC and HMBC) and comparison with literature reports, the structure of this solid was shown to be bergenin, a dihydroisocoumarin derivative of glucopyranosyl gallic acid.

Separation Methods of Phenolic Compounds from Plant Extract as Antioxidant Agents Candidate

“…Dummy templates have an analogous structure to the target analyte molecules, with similar shape, size, and functional group, but they do not interfere with analytical determinations [236,237]. Eidi et al [86] developed a dummy template molecularly imprinted polymer (DMIP) for the selective isolation of sesquiterpene coumarins from asafoetida. They used 7-hydroxycomarin (7-HC), the parent compound of sesquiterpene coumarins, as a dummy template.…”