Isolation and Characterisation of Alongshan Virus in Russia

Kholodilov, Ivan S.; Litov, Alexander G.; Klimentov, Alexander S.; Belova, Oxana A.; Polienko, Alexandra E.; Nikitin, Nikolai A.; Щетинин, А. М.; Ivannikova, Anna Y.; Bell‐Sakyi, Lesley; Yakovlev, Alexander S.; Bugmyrin, Sergey; Bespyatova, L. A.; Gmyl, Larissa V.; Luchinina, Svetlana V.; Gmyl, Anatoly P.; Gushchin, Vladimir A.; Karganova, Galina G.

doi:10.3390/v12040362

Cited by 50 publications

(64 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Medium was changed at weekly intervals by removal and replacement of 1.1 mL; the spent medium was used to harvest the virus, as described below. Subcultures were carried out occasionally, as described previously [ 7 ].…”

Section: Methodsmentioning

confidence: 99%

“…Reverse transcription was performed with random hexamer primers (R6) and M-MLV reverse transcriptase (Promega, Madison, WI, USA), according to the manufacturer’s protocols. Viral genomic cDNA was amplified by PCR using primers for the genus Flavivirus [ 33 ] and specific primers for ALSV: Alongshan_seg1_5 and Miass_NS5_1R for segment 1 ( Table S3 ) and Miass_gly_3F and Miass_gly_3R for segment 2 [ 7 ]. Sequencing was carried out in both directions directly from PCR-amplified DNA on the ABI PRISM 3730 (Applied Biosystems, Foster City, CA, USA) sequencer using ABI PRISM ® BigDye™ Terminator v. 3.1.…”

Section: Methodsmentioning

confidence: 99%

“…The representatives of the Jingmenvirus (JMV) group, such as the Jingmen tick virus (JMTV), Alongshan virus (ALSV), Yanggou tick virus (YGTV) and others, have segmented ssRNA(+) genomes, two segments of which have homology with the well-studied flavivirus RNA-dependent RNA polymerase NS5 (segment 1) and RNA helicase-protease NS3 (segment 3) [ 3 , 5 ]. The remaining two segments are specific only to JMVs and encode glycoproteins—a novel upstream open reading frame (nuORF), VP1a and VP1b proteins (segment 2) [ 5 , 6 , 7 ] and other virus proteins—VP2 and VP3 (segment 4) [ 2 , 3 , 5 ]. Excluding plant viruses, before the discovery of JMTVs [ 3 ], only representatives of the genus Omegatetravirus of the family Tetraviridae , which infect insects [ 8 ], and the genera Alphanodavirus and Betanodavirus of the family Nodaviridae , which infect insects and fish, respectively [ 9 ], were known to have a segmented ssRNA(+) genome.…”

Section: Introductionmentioning

confidence: 99%

“…Representatives of the JMV group are found on almost all continents: Eurasia [ 2 , 3 , 5 , 7 , 10 , 11 , 12 , 13 ], Africa [ 11 , 14 ], South America [ 11 , 14 , 15 , 16 , 17 ] and North America [ 18 ]. RNA of this virus group has been detected in different species of insects (fruit flies [ 19 ] and mosquitoes [ 20 ]), ticks [ 2 , 3 , 5 , 7 , 10 , 11 , 13 , 17 , 20 ] and mammals [ 3 , 11 , 14 , 15 , 21 ], including humans [ 10 , 12 , 20 ].…”

Section: Introductionmentioning

confidence: 99%

“…The first detection of ALSV occurred in China in a patient with fever of unknown aetiology. Later, this virus was detected in mosquitoes [ 20 ], ticks [ 7 , 11 , 13 , 20 ] and mammals [ 20 , 22 ]. We showed, for the first time, that the virus can reproduce and cause persistent infection in tick cell cultures [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Geographical and Tick-Dependent Distribution of Flavi-Like Alongshan and Yanggou Tick Viruses in Russia

Kholodilov

Belova

Morozkin

et al. 2021

Viruses

Self Cite

View full text Add to dashboard Cite

The genus Flavivirus includes related, unclassified segmented flavi-like viruses, two segments of which have homology with flavivirus RNA-dependent RNA polymerase NS5 and RNA helicase-protease NS3. This group includes such viruses as Jingmen tick virus, Alongshan virus, Yanggou tick virus and others. We detected the Yanggou tick virus in Dermacentor nuttalli and Dermacentor marginatus ticks in two neighbouring regions of Russia. The virus prevalence ranged from 0.5% to 8.0%. We detected RNA of the Alongshan virus in 44 individuals or pools of various tick species in eight regions of Russia. The virus prevalence ranged from 0.6% to 7.8%. We demonstrated the successful replication of the Yanggou tick virus and Alongshan virus in IRE/CTVM19 and HAE/CTVM8 tick cell lines without a cytopathic effect. According to the phylogenetic analysis, we divided the Alongshan virus into two groups: an Ixodes persulcatus group and an Ixodes ricinus group. In addition, the I. persulcatus group can be divided into European and Asian subgroups. We found amino acid signatures specific to the I. ricinus and I. persulcatus groups and also distinguished between the European and Asian subgroups of the I. persulcatus group.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Geographical and Tick-Dependent Distribution of Flavi-Like Alongshan and Yanggou Tick Viruses in Russia

Kholodilov

Belova

Morozkin

et al. 2021

Viruses

Self Cite

View full text Add to dashboard Cite

show abstract

Characterisation of putative novel tick viruses and zoonotic risk prediction

Lin,

Pascall

2024

Ecology and Evolution

View full text Add to dashboard Cite

Tick‐associated viruses remain a substantial zoonotic risk worldwide, so knowledge of the diversity of tick viruses has potential health consequences. Despite their importance, large amounts of sequences in public data sets from tick meta‐genomic and ‐transcriptomic projects remain unannotated, sequence data that could contain undocumented viruses. Through data mining and bioinformatic analysis of more than 37,800 public meta‐genomic and ‐transcriptomic data sets, we found 83 unannotated contigs exhibiting high identity with known tick viruses. These putative viral contigs were classified into three RNA viral families (Alphatetraviridae, Orthomyxoviridae and Chuviridae) and one DNA viral family (Asfarviridae). After manual checking of quality and dissimilarity towards other sequences in the data set, these 83 contigs were reduced to five contigs in the Alphatetraviridae from four putative viruses, four in the Orthomyxoviridae from two putative viruses and one in the Chuviridae which clustered with known tick‐associated viruses, forming a separate clade within the viral families. We further attempted to assess which previously known tick viruses likely represent zoonotic risks and thus deserve further investigation. We ranked the human infection potential of 133 known tick‐associated viruses using a genome composition‐based machine learning model. We found five high‐risk tick‐associated viruses (Langat virus, Lonestar tick chuvirus 1, Grotenhout virus, Taggert virus and Johnston Atoll virus) that have not been known to infect human and two viral families (Nairoviridae and Phenuiviridae) that contain a large proportion of potential zoonotic tick‐associated viruses. This adds to the knowledge of tick virus diversity and highlights the importance of surveillance of newly emerging tick‐associated diseases.

show abstract

The Tick-Borne Pathogens: An Overview of China’s Situation

et al. 2023

View full text Add to dashboard Cite

Background Ticks are important medical arthropods that can transmit hundreds of pathogens, such as parasites, bacteria, and viruses, leading to serious public health burdens worldwide. Unexplained fever is the most common clinical manifestation of tick-borne diseases. Since the emergence of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), the surge of coronavirus disease 2019 (COVID-19) cases led to the hospital overload and fewer laboratory tests for tick-borne diseases. Therefore, it is essential to review the tick-borne pathogens and further understand tick-borne diseases. Purpose The geographic distribution and population of ticks in the Northern hemisphere have expanded while emerging tick-borne pathogens have been introduced to China continuously. This paper focused on the tick-borne pathogens that are threatening public health in the world. Their medical significant tick vectors, as well as the epidemiology, clinical manifestations, diagnosis, treatment, prevention, and control measures, are emphasized in this document. Methods In this study, all required data were collected from articles indexed in English databases, including Scopus, PubMed, Web of Science, Science Direct, and Google Scholar. Results Ticks presented a great threat to the economy and public health. Although both infections by tick-borne pathogens and SARS-CoV-2 have fever symptoms, the history of tick bite and its associated symptoms such as encephalitis or eschar could be helpful for the differential diagnosis. Additionally, as a carrier of vector ticks, migratory birds may play a potential role in the geographical expansion of ticks and tick-borne pathogens during seasonal migration. Conclusion China should assess the risk score of vector ticks and clarify the potential role of migratory birds in transmitting ticks. Additionally, the individual and collective protection, vector control, comprehensive surveillance, accurate diagnosis, and symptomatic treatment should be carried out, to meet the challenge. Graphical Abstract

show abstract

Isolation and Characterisation of Alongshan Virus in Russia

Cited by 50 publications

References 41 publications

Geographical and Tick-Dependent Distribution of Flavi-Like Alongshan and Yanggou Tick Viruses in Russia

Geographical and Tick-Dependent Distribution of Flavi-Like Alongshan and Yanggou Tick Viruses in Russia

Characterisation of putative novel tick viruses and zoonotic risk prediction

The Tick-Borne Pathogens: An Overview of China’s Situation

Contact Info

Product

Resources

About