Isolation and Characterisation of a Reserve Protein from the Seeds of Cereus jamacaru (Cactaceae)

Costa, Itayguara Ribeiro da; Souza, P.; Bloch, Carlos; Llamoca-Zárate, Rómulo M.; Campos, F. A. P.

doi:10.1590/s1516-89132001000400002

Cited by 7 publications

(3 citation statements)

References 10 publications

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“…Ep‐AMP1 is the first ICK peptide reported from the Cactaceae family. Indeed, few peptides have previously been described from this plant family: a methionine‐rich polypeptide from seeds of Cereus jamacaru ,30 an arginine‐rich polypeptide (48 residues, unsequenced) from Opuntia ficus‐indica 31 and a trypsin inhibitory peptide (38 residues) from Opuntia streptacantha . Compared to other ICK peptides, Ep‐AMP1 has high structural similarity to spider toxins, and lacks the chitin‐binding properties of peptides with the same sequence pattern (i.e., plant C6‐type antimicrobial peptides).…”

Section: Discussionmentioning

confidence: 99%

A Cactus‐Derived Toxin‐Like Cystine Knot Peptide with Selective Antimicrobial Activity

et al. 2015

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Naturally occurring cystine knot peptides show a wide range of biological activity, and as they have inherent stability they represent potential scaffolds for peptide-based drug design and biomolecular engineering. Here we report the discovery, sequencing, chemical synthesis, three-dimensional solution structure determination and bioactivity of the first cystine knot peptide from Cactaceae (cactus) family: Ep-AMP1 from Echinopsis pachanoi. The structure of Ep-AMP1 (35 amino acids) conforms to that of the inhibitor cystine knot (or knottin) family but represents a novel diverse sequence; its activity was more than 500 times higher against bacterial than against eukaryotic cells. Rapid bactericidal action and liposome leakage implicate membrane permeabilisation as the mechanism of action. Sequence homology places Ec-AMP1 in the plant C6-type of antimicrobial peptides, but the three dimensional structure is highly similar to that of a spider neurotoxin.

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Section: Discussionmentioning

confidence: 99%

A Cactus‐Derived Toxin‐Like Cystine Knot Peptide with Selective Antimicrobial Activity

et al. 2015

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“…Medicinal properties have been attributed to the mandacaru, for example, as infusion for treatment of various diseases, including renal, hepatic, respiratory, stomach, and sinus problems (Agra et al 2007;Albuquerque et al 2007;Saraiva et al 2015;Silva et al 2014). Other studies have reported the cytotoxic activity of an aqueous extract (non-protein) from the stem (Silva 2015); antibacterial activity of ethanolic extract from the stem (Davet et al 2009); as well as the purification and characterization of two proteins from C. jamacaru seeds (Aragão et al 2000;da Costa et al 2001).…”

Section: Popular Use and Possible Applicationmentioning

confidence: 99%

Food Plants in the Caatinga

et al. 2021

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“…The seeds by weight and volume of molar solution were used by 1:4 ratios. Then this supernatant was subjected to 12000 rpm for 20 min at 4°C and again the supernatant was collected for further processing (Costa et al 2001).…”

Section: Extract Preparationmentioning

confidence: 99%

Antidiabetic, Antibacterial and Antioxidant Potential of Bioactive Peptides Extracted from the Germinated Seeds of Poncirus trifoliata

Mehmood¹

2021

IJAB

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The seeds proteins are converted into the peptides during the germination through peptidases. These bioactive peptides are fractionated through the enzymes. The bioactive peptides are reported with the number of therapeutic effects like antioxidant, antibacterial, antidiabetic, anti-cancerous and antihypertensive etc. Synthetic drugs have number of adverse effects along with the therapeutic effects, but natural sources are safer and free from this problem. In the present research, Poncirus trifoliata seedling peptides extracts were used to determine antibacterial, antioxidant and anti-diabetic activities. The peptides extract showed substantial growth of inhibition against pathogenic strains including Escherichia coli, Staphylococcus aureus and Bacillus subtilis having the superior effect as compared to amoxicillin antibiotic as a standard drug. The IC50 value of P. trifoliata seeds peptides extract was 32.91 μg mL-1 as compared with standard (IC50) value 25.52 μg mL-1 of ascorbic acid showed good potential as antioxidant activity. The extracted germinated seeds peptides showed considerable antidiabetic activity in controlling the blood glucose level of alloxan induced diabetic rabbits to compare with standard drugs like glibenclamide. Thus, this study confirmed the antibacterial, antioxidant and antidiabetic effects of P. trifoliata seeds peptides is significantly higher as compared to pure drugs. Therefore, further complete separation and detailed investigation study is needed to isolate target peptides and revealed mechanism of action of bioactive peptides and formulation as a drug to replace the synthetics drugs with safe one. © 2021 Friends Science Publishers

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Isolation and Characterisation of a Reserve Protein from the Seeds of Cereus jamacaru (Cactaceae)

Cited by 7 publications

References 10 publications

A Cactus‐Derived Toxin‐Like Cystine Knot Peptide with Selective Antimicrobial Activity

A Cactus‐Derived Toxin‐Like Cystine Knot Peptide with Selective Antimicrobial Activity

Food Plants in the Caatinga

Antidiabetic, Antibacterial and Antioxidant Potential of Bioactive Peptides Extracted from the Germinated Seeds of Poncirus trifoliata

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