Isolation and characterisation of a novel dehydration-induced Grp94 homologue from the resurrection plant Xerophyta viscosa

Walford, Sally-Ann; Thomson, Jennifer A.; Farrant, Jill M.; Musil, C.F.

doi:10.1016/s0254-6299(15)30175-7

Cited by 8 publications

(4 citation statements)

References 47 publications

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“…Like to leaves, the levels of stress-responsive proteins [Grp94 (HSP, R14) and disease resistance protein (R70)] in roots increased in response to Mg-deficiency (Table 2 ). This agrees with the reports that B-deficiency increased the level of NB-ARC domain-containing disease resistance protein in C. sinensis roots [ 16 ], and that the abundance of Grp94 in Xerophyta viscosa plants increased in response to heat and dehydration [ 50 ]. However, the abundances of mitochondrial heat shock 70 kDa protein (R53) and heat shock protein HSP26 (R100) were decreased in Mg-deficient roots (Table 2 ), which agrees with the studies that B-deficiency decreased the levels of several HSPs (HSP70, HSP70 family proteins, mitochondrial HSO70 2 and HSP10) in C. sinensis roots [ 16 ] and the abundances of three HSPs in Lupinus albus roots [ 51 ].…”

Section: Discussionsupporting

confidence: 91%

Proteomic analysis of Citrus sinensis roots and leaves in response to long-term magnesium-deficiency

Peng

Lee

et al. 2015

BMC Genomics

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BackgroundMagnesium (Mg)-deficiency is frequently observed in Citrus plantations and is responsible for the loss of productivity and poor fruit quality. Knowledge on the effects of Mg-deficiency on upstream targets is scarce. Seedlings of ‘Xuegan’ [Citrus sinensis (L.) Osbeck] were irrigated with Mg-deficient (0 mM MgSO4) or Mg-sufficient (1 mM MgSO4) nutrient solution for 16 weeks. Thereafter, we first investigated the proteomic responses of C. sinensis roots and leaves to Mg-deficiency using two-dimensional electrophoresis (2-DE) in order to (a) enrich our understanding of the molecular mechanisms of plants to deal with Mg-deficiency and (b) understand the molecular mechanisms by which Mg-deficiency lead to a decrease in photosynthesis.ResultsFifty-nine upregulated and 31 downregulated protein spots were isolated in Mg-deficient leaves, while only 19 upregulated and 12 downregulated protein spots in Mg-deficient roots. Many Mg-deficiency-responsive proteins were involved in carbohydrate and energy metabolism, followed by protein metabolism, stress responses, nucleic acid metabolism, cell wall and cytoskeleton metabolism, lipid metabolism and cell transport. The larger changes in leaf proteome versus root one in response to Mg-deficiency was further supported by our observation that total soluble protein concentration was decreased by Mg-deficiency in leaves, but unaffected in roots. Mg-deficiency had decreased levels of proteins [i.e. ribulose-1,5-bisphosphate carboxylase (Rubisco), rubisco activase, oxygen evolving enhancer protein 1, photosynthetic electron transfer-like protein, ferredoxin-NADP reductase (FNR), aldolase] involved in photosynthesis, thus decreasing leaf photosynthesis. To cope with Mg-deficiency, C. sinensis leaves and roots might respond adaptively to Mg-deficiency through: improving leaf respiration and lowering root respiration, but increasing (decreasing) the levels of proteins related to ATP synthase in roots (leaves); enhancing the levels of proteins involved in reactive oxygen species (ROS) scavenging and other stress-responsive proteins; accelerating proteolytic cleavage of proteins by proteases, protein transport and amino acid metabolism; and upregulating the levels of proteins involved in cell wall and cytoskeleton metabolism.ConclusionsOur results demonstrated that proteomics were more affected by long-term Mg-deficiency in leaves than in roots, and that the adaptive responses differed between roots and leaves when exposed to long-term Mg-deficiency. Mg-deficiency decreased the levels of many proteins involved in photosynthesis, thus decreasing leaf photosynthesis.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-015-1462-z) contains supplementary material, which is available to authorized users.

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Section: Discussionsupporting

confidence: 91%

Proteomic analysis of Citrus sinensis roots and leaves in response to long-term magnesium-deficiency

Peng

Lee

et al. 2015

BMC Genomics

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“…In loblolly pine GRP94 was highly expressed under severe water stress condition as well as after rehydration (Vasquez-Robinet et al 2010). In resurrection plant Xerophyta viscose GRP94 was significantly induced by dehydration and heat stress and slightly by salt stress but not induced by cold, ABA and high light (Walford et al 2004). Protein aggregates can be efficiently resolubilized by HSP100/Clp family chaperones and then refolded by the HSP70 and HSP60 family chaperones (Ben-Zvi and Goloubinoff 2001).…”

Section: Heat Shock Proteinsmentioning

confidence: 99%

Genome-wide transcriptomic analysis of cotton under drought stress reveal significant down-regulation of genes and pathways involved in fibre elongation and up-regulation of defense responsive genes

et al. 2011

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Cotton is an important source of natural fibre used in the textile industry and the productivity of the crop is adversely affected by drought stress. High throughput transcriptomic analyses were used to identify genes involved in fibre development. However, not much information is available on cotton genome response in developing fibres under drought stress. In the present study a genome wide transcriptome analysis was carried out to identify differentially expressed genes at various stages of fibre growth under drought stress. Our study identified a number of genes differentially expressed during fibre elongation as compared to other stages. High level up-regulation of genes encoding for enzymes involved in pectin modification and cytoskeleton proteins was observed at fibre initiation stage. While a large number of genes encoding transcription factors (AP2-EREBP, WRKY, NAC and C2H2), osmoprotectants, ion transporters and heat shock proteins and pathways involved in hormone (ABA, ethylene and JA) biosynthesis and signal transduction were up-regulated and genes involved in phenylpropanoid and flavonoid biosynthesis, pentose and glucuronate interconversions and starch and sucrose metabolism pathways were down-regulated during fibre elongation. This study showed that drought has relatively less impact on fibre initiation but has profound effect on fibre elongation by down-regulating important genes involved in cell wall loosening and expansion process. The comprehensive transcriptome analysis under drought stress has provided valuable information on differentially expressed genes and pathways during fibre development that will be useful in developing drought tolerant cotton cultivars without compromising fibre quality.

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“…The two Nuclear/cytosolic HSPs, GmDjp1 and DNAj in soybean, have been associated with abiotic [142] and biotic [141] stress tolerance. Proteins present in endoplasmic reticulum (ER) HSP90s is also named as glucose-regulated protein 94 (GRP94), which is responsible for drought stress tolerance in Xerophyta viscosa, a drought-tolerant plant [145]. Another ER-resident Hsp70s known as BiPs (luminal Binding Proteins) the overexpression of SoyBiPD (soybean BiPs) conferred drought stress tolerance in tobacco.…”

Section: Heat Shock Proteins (Hsps)mentioning

confidence: 99%

Approaches for the amelioration of adverse effects of drought stress on crop plants

Dubey

Kumar

Malla

et al. 2021

Front Biosci (Landmark Ed)

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Isolation and characterisation of a novel dehydration-induced Grp94 homologue from the resurrection plant Xerophyta viscosa

Cited by 8 publications

References 47 publications

Proteomic analysis of Citrus sinensis roots and leaves in response to long-term magnesium-deficiency

Proteomic analysis of Citrus sinensis roots and leaves in response to long-term magnesium-deficiency

Genome-wide transcriptomic analysis of cotton under drought stress reveal significant down-regulation of genes and pathways involved in fibre elongation and up-regulation of defense responsive genes

Approaches for the amelioration of adverse effects of drought stress on crop plants

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