Isolation and characterisation of a novel rhabdovirus from snakehead fish showing surface ulcerations and mortality from India: First report

John, K. Riji; George, M. Rosalind; Mageshkumar, Paulraj; Mansoor, Mohideenpitchai Mohamed; Devaraj, K.V.; Sivasankar, P; Selvamagheswaran, M.; Petchimuthu, M.

doi:10.1016/j.aquaculture.2021.737120

Cited by 7 publications

(5 citation statements)

References 25 publications

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“…The pathological lesions observed in the infected fish, including hemorrhages and focal necrosis in the liver, spleen, and kidney, were similar to those reported in farmed Japanese flounder snakehead fish (Channa striata) [36,[44][45][46][47]. In situ hybridization showed that CAPRV2023 was distributed in different tissues, with a higher positive signal intensity in the spleen, liver, and muscle tissues of golden pompano, which was different from that of VHSV in juvenile herring.…”

Section: Discussionsupporting

confidence: 73%

Mass mortality associated with Carpione Rhabdovirus in golden pompano (Trachinotus ovatus) in China: First report

Sun,

Huang,

Wang

et al. 2024

Preprint

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A severe epizootic outbreak occurred in a deep-water cultured golden pompano (Trachinotus ovatus) in Guangdong Province, China (August–November 2023); the cumulative mortality was 65–82%. The diseased fish showed clinical signs of lethargy, anorexia, whirling movements, and hemorrhage at the base of the fins and in the upper and lower jaws before mass mortality. A Rhabdovirus strain, CARPV2023, was successfully isolated using FHM cells. Cytopathic effects of the tissue filtrate of the diseased golden pompano on FHM and EPC cell monolayers were characterized by rounded cells, grape-like cluster formation, detachment, and lysis. Histopathology revealed congestion, hemorrhage, and necrosis in the spleen, liver, kidney, and intestinal tissues of naturally and experimentally infected golden pompanos. Electron microscopy showed that bullet-shaped viral particles 183.5–201.6-nm long and 57.3–82.7-nm wide budded from cell membrane or aggregated around the infected FHM cells. The virus remained stable for 7 days at 4–33 °C and grew optimally at 28 °C. Whole-genome sequencing and phylogenetic analysis based on the full genome further indicated that the CARPV2023 strain is a member of Carpione Rhabdovirus, which is related to Novirhabdovirus with unclassification. A specific nested reverse transcriptase-polymerase chain reaction confirmed the presence of CARPV in the affected golden pompano. Much higher mortality was observed in challenged golden pompanos than in the controls through intraperitoneal injection and immersion infection. In situ hybridization showed positive reactions in the fish tissues. This is the first study to report natural CARPV infections in marine fish in the world.

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Section: Discussionsupporting

confidence: 73%

Mass mortality associated with Carpione Rhabdovirus in golden pompano (Trachinotus ovatus) in China: First report

Sun,

Huang,

Wang

et al. 2024

Preprint

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“…The samples were then dehydrated in 70%–100% alcohol, placed twice in xylene (10 min each), and embedded in paraffin (56°C melting point). The parts were sliced transversely into 4‐μm‐thick sections using a microtome (Thermo Shandon, UK) and stained with haematoxylin and eosin (John et al, 2021). Then the stained slides were analysed and photographed by Nikon microscope (Nikon, Japan) installed with a digital camera.…”

Section: Methodsmentioning

confidence: 99%

“…and stained with haematoxylin and eosin (John et al, 2021). Then the stained slides were analysed and photographed by Nikon microscope (Nikon, Japan) installed with a digital camera.…”

Section: Histopathology Of Experimentally Infected Fishmentioning

confidence: 99%

Pathogenicity potential of antimicrobial‐resistant Photobacterium damselae subsp. damselae isolated from maricultured and wild‐caught fish of the southeast coast of India

Petchimuthu

George

John

et al. 2022

Aquaculture Research

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Antimicrobial resistance and zoonotic pathogen transmission from wild fish to high‐density cage‐cultured fish are two of the emerging concerns in mariculture. Photobacterium damselae is one of the most destructive bacterial pathogens which is responsible for huge economic loss to the cage culture systems. To investigate the above, both wild‐caught and cage‐cultured fish were collected from the southeast coast of India. P. damselae subsp. damselae was isolated and their genotypic profiling was done using a specific multiplex polymerase chain reaction. Out of 280 isolates, 61 isolates were confirmed as P. damselae subsp. damselae. The overall prevalence of P. damselae subsp. damselae in wild fish was significantly higher compared with cage‐cultured fish. Among the wild fish, parrot fish showed more prevalence when compared with other fish. Around 90% of the identified bacterial isolates were multiple antibiotic‐resistant and carried antibiotic resistance genes such as beta‐lactam and streptomycin genes. To know the virulence of P. damselae bacteria isolated from wild‐caught fish to commercially important cage‐cultured fish (cobia and silver pompano) experimentally, a challenging study was carried out. The results revealed that cobia and silver pompano showed 100% and 85% mortality at a bacterial concentration of 3.5 × 104 CFU Fish−1 and 2.75 × 106 CFU Fish−1 respectively. Severe tissue damage and ulceration were also noticed in both fish. In conclusion, the present study found a high rate of AMR in P. damselae subsp. damselae isolated from the fish of the southeast coast of India that exhibit the strong pathogenic potential to cage‐cultured fish.

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“…Recently, isolation of a snakehead fish vesiculovirus (SHVV) was reported from hybrid catfish in China [85]. First report of emergence of a rhabdovirus (SHRV In) in India was from an infected snakehead that were undergoing mortality in the extensive type aquaculture tanks in the rural areas of South India that had behavioral abnormalities and surface ulcerations (Figure 14) [86]. Experimental infection with SHRV-In exhibited clinical signs of listlessness, uncoordinated spiral swimming, anorexia, lethargy, loss of scales, discoloration, small pale white patches on the body, hemorrhagic areas, and surface ulcers in juvenile snakeheads.…”

Section: Transmission Electron Micrographs Of Snakehead Rhabdovirus P...mentioning

confidence: 99%

“…Experimental infection with SHRV-In exhibited clinical signs of listlessness, uncoordinated spiral swimming, anorexia, lethargy, loss of scales, discoloration, small pale white patches on the body, hemorrhagic areas, and surface ulcers in juvenile snakeheads. Internally, fish showed hemorrhages and ascitic fluid [86]. Status of Emerging and Reemerging Fish Viral Infections in India DOI: http://dx.doi.org/10.5772/intechopen.109012…”

Section: Transmission Electron Micrographs Of Snakehead Rhabdovirus P...mentioning

confidence: 99%

Status of Emerging and Reemerging Fish Viral Infections in India

John¹,

Sivasankar²,

George³

2023

Viral Outbreaks - Global Impact and Newer Horizons

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Aquaculture, being the fastest growing food production sector, has now become vital to the socioeconomic development of many countries. In India, aquaculture plays a significant role in food production, ensuring nutritional security, boosting agricultural exports, and generating job opportunities. The production of farmed fish has greatly expanded qualitatively and quantitatively in both freshwater and marine water regimes to fulfill the ever-growing demand. However, the occurrence of diseases is the main obstacle to sustainable aquaculture production, which has an impact on the socioeconomic status of fish farmers of the country. Viral diseases inflict irreparable damage to the aquaculture enterprise causing large-scale economic losses and ecological problems. Recently, there has been a spike in the incidence of new emerging viral diseases in diverse species of aquaculture species. Prophylactics by far being the only feasible method of viral disease control, the development of viral vaccines is highly imperative. A precise understanding of the disease pathology, etiological agent, and species susceptible to the specific diseases are highly essential in this perspective. The chapter highlights the emerging and reemerging viral diseases in the Indian aquaculture sector.

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Isolation and characterisation of a novel rhabdovirus from snakehead fish showing surface ulcerations and mortality from India: First report

Cited by 7 publications

References 25 publications

Mass mortality associated with Carpione Rhabdovirus in golden pompano (Trachinotus ovatus) in China: First report

Mass mortality associated with Carpione Rhabdovirus in golden pompano (Trachinotus ovatus) in China: First report

Pathogenicity potential of antimicrobial‐resistant Photobacterium damselae subsp. damselae isolated from maricultured and wild‐caught fish of the southeast coast of India

Status of Emerging and Reemerging Fish Viral Infections in India

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