Isolation and callus formation of Gracilariopsis bailiniae (Gracilariales, Rhodophyta) protoplasts

Single-cell transcriptome sequencing revealing the difference in photosynthesis and carbohydrate metabolism between epidermal cells and non-epidermal cells of Gracilariopsis lemaneiformis (Rhodophyta)

“…Then, the tissues were incubated in mixed enzymes solution (2% w/v cellulase, 1% w/v macerozyme R-10, 0.8 M sorbitol, 600 μL of crude Marinomonas sp. YS-70 agarase solution, and 400 μL of deionized water) for 1 h at 27°C ( Chen et al., 2018 ). After that, the ECs were isolated from the epidermal tissues using a 10 μm filter screen and collected from the filtrate following centrifugation (200 ×g for 8 min).…”

Estimating the ploidy of Gracilariopsis lemaneiformis at both the cellular and genomic level1

“…Approximately 20 fragments were incubated in 1 mL of mixed enzyme solution (deionized water: crude Marinomonas sp. YS‐70 agarase solution = 2:3, containing 0.01 mmol · L ‐1 phosphate‐buffered saline (PBS) with pH 6.5, 2% w/v cellulose; C1794, Sigma, USA and 1% w/v macerozyme R‐10; Yuanye, Shanghai, China) for 1 h at 27°C with some modifications (Chen et al 2018). The fragments were then rinsed three times with deionized water and fixed in 3 mL Carnoy fixative (ethanol/acetic acid, 3:1) overnight at 4°C.…”

A new neoagarobiose-producing agarase from Vibrio sp. LA1

“…Similarly, NAOS possess diverse biological activities, such as moisturizing effects and whitening effects on skin [7,8], anti-oxidant activities [9][10][11], prebiotic effects [11,12], and potential treatment on type II diabetes [13]. It has been recognized that besides being mainly used in NAOS production, agarases can also be used in recovery of DNA from agarose gel [14,15], protoplast preparation of from red alga [16,17], and the screening of signal peptides [15]. All these applications rely on the search and the large-scale production of potent agarases.…”