Isolation and biochemical characterization of heavy-metal resistant bacteria from tannery effluent in Chittagong city, Bangladesh: Bioremediation viewpoint

Marzan, Lolo Wal; Hossain, Mehjabeen; Mina, Sohana Akter; Akter, Yasmin; Chowdhury, A. M. Masudul Azad

doi:10.1016/j.ejar.2016.11.002

Cited by 160 publications

(71 citation statements)

References 22 publications

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“…and Micrococcus sp. showed resistance to heavy metals like lead (Pb), chromium (Cr) and cadmium (Cd) [18]. CMG 457 was highly resistant to the pesticides ripcord and thiodon.…”

Section: Discussionmentioning

confidence: 99%

Indigenous Oil Degrading Bacteria: Isolation, Screening and Characterization

Shahab

Shafi

Ahmed

2017

Natl. j. health sci.

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Background: Oil contamination is increasing at an alarming rate and to overcome to this problem the most efficient method used is biodegradation. Biodegradation is a natural mechanism that can be used effectively for degradation of recalcitrant hydrocarbon pollutants prevailed in the environment by employing environmentally friendly microbes.Methods: Strain CMG 457 was isolated from petrol pump site from Firdous Colony, Karachi and was identified as Enterobacter sp. after morphological and biochemical characterization. Strain resistance towards different organic hydrocarbons (paraffin, aromatics and pesticides) and utilization of various organic compounds as sole carbon source were analyzed on Nutrient agar. MIC of antibiotics and heavy metals was checked on Bushnell Haas (BH) agar at 37°C for 24 hrs. Results:Research investigated the role of Enterobacter sp. in biodegradation of petroleum hydrocarbons. The results showed that the bacterium is a gram-negative aerobic bacillus. Enterobacter sp. designated as CMG 457 was screened for oil degradation using BH agar. Resistance of strain against different organic hydrocarbons was tested and CMG 457 showed high resistance of upto 50% against paraffin. The Minimum Inhibitory Concentration (MIC) of heavy metals and antibiotic sensitivity were investigated for bacterial strains. Screening for antibiotic resistance revealed that CMG 457 was resistant to ampicillin and erythromycin and was able to tolerate heavy metals like CuSO 4 , CdCl 2 and CrCl 2 upto 3mM. Conclusions:From the study, it is concluded that oil contaminated areas are the best source for isolation of hydrocarbon degrading bacteria. Enterobacter sp. has a potential for oil degradation as it showed better hydrocarbon degrading ability.

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“…and Micrococcus sp. showed resistance to heavy metals like lead (Pb), chromium (Cr) and cadmium (Cd) [18]. CMG 457 was highly resistant to the pesticides ripcord and thiodon.…”

Section: Discussionmentioning

confidence: 99%

Indigenous Oil Degrading Bacteria: Isolation, Screening and Characterization

Shahab

Shafi

Ahmed

2017

Natl. j. health sci.

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“…From the contaminated area, two bacterial isolates, Gemella sp. and Micrococcus sp., which were identified based on morphological, cultural, physiological and biochemical characteristics, showed resistance against Lead, chromium and cadmium (Marzan et al, 2017). Although the tested cave bacteria have not exposed to anthropogenic antibiotics before, isolates of Pseudomonas earuginosa were the most resistance to all tested antibiotics; percentages of resistance were 91, 64, 54 and 43 % for Ampicillin, Streptomycin, Tetracycline and Neomycin respectively.…”

Section: Name Of Premiersmentioning

confidence: 99%

Microbial Diversity and Phylogenetic Studies of Some Microbes Obtained From Unexplored Caves of Saudi Arabia

Amasha¹

2018

JEBAS

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The microbial diversity within cave ecosystems is largely unknown. This study aimed to studying the microbial communities of the three caves viz Mossy, Hotel and Reda caves which are located approximately 200 km of Riyadh region, between Riyadh and Al Kharj road, Saudi Arabia. These caves have an intricate cave system which developed in the calcareous sandstone and clay. Morphological interpretation revealed that Mossy cave was different from Hotel and Reda caves where it is tall and have a linear cave passage and narrow canyons. Various field studies revealed that many bacteria inhibited in caves and obtained their energy from degradation of inorganic substances, thus they directly interact with the surfaces where they live on. In present study, soil and wall dust samples were collected and cultured on Nutrient agar, starch nitrate agar and PDA plates. During study various Gram positive, Gram negative bacteria, actinomycetes and fungi were isolated from the cave soil and wall. The isolated bacteria were characterized and identified by using culture-dependent methods (morphological and physiological methods). Identifications of the most occurring bacterial genera were confirmed by using 16S rRNA gene amplicon sequencing which revealed the presence of 11 broad taxonomic species of bacteria. Among these, Proteobacteria were dominant in all caves and this was followed by Actinobacteria, Firmicutes and Bacteroidetes. Majority of the true bacterial isolates belong to the genera Alcaligenes, Bacillus, Brevibacterium, Klebsiella, Planomicrobium, Shigella, Pseudomonas and Staphylococcus. During study, percentage of resistance to some metallic cations, heavy metals and antibiotics were also determined. Result of study revealed that Bacillus cereus, Klebsiella pneumoniae

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“…Depending on gram staining, three isolates (W, PW and Y) were identified as gram positive bacteria (Table 1) by detecting peptidoglycan which is present in a thick layer in bacteria (Marzan et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Characterization, identification and antibiogram studies of endophytic bacteria from cowpea [Vigna unguiculata (L.) Walp]

Marzan

Alam

Hossain³

2018

Bangladesh J. Agric. Res

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This study included isolation, characterization and identification of endophytic bacteria from cowpea [Vigna unguiculata (L.) Walp] roots from Hathazari upazilla, Chittagong, Bangladesh and three specis of bacteria, Staphylococcus intermedius, Staphylococcus caprae and Staphylococcus saprophyticus were finally identified based on their morphological and biochemical characteristics according to established protocol. Results of antibiotic sensitivity pattern of those endophytic bacteria suggested that they can be utilized as a cost-effective biological control agent in future applications, such as delivery of enzymes for controlling certain plant diseases against various pathogens. The findings of this study may help to determine the potential application of those endophytic bacteria in biotechnology, medicine and agriculture.

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Isolation and biochemical characterization of heavy-metal resistant bacteria from tannery effluent in Chittagong city, Bangladesh: Bioremediation viewpoint

Cited by 160 publications

References 22 publications

Indigenous Oil Degrading Bacteria: Isolation, Screening and Characterization

Indigenous Oil Degrading Bacteria: Isolation, Screening and Characterization

Microbial Diversity and Phylogenetic Studies of Some Microbes Obtained From Unexplored Caves of Saudi Arabia

Characterization, identification and antibiogram studies of endophytic bacteria from cowpea [Vigna unguiculata (L.) Walp]

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