Isolation and Assessment of Plant Growth Promoting Activity of Siderophore ProducingPseudomonas fluorescensin Crops

Abstract

The Pseudomonas fluorescens a major Rhizobacteria encourage the plant growth through producing yellowish green fluroscent siderophore involve in high affinity transport of iron into the cell. The P. fluorescens isolates grow in iron deficient media because of production capacity of siderophore depends on iron content. Fifty nine Pseudomonas fluorescens were isolated in King's B media under 260 nm wavelengths from the rhizosphere and non-rhizospheric soil of cave, forest, fellow land and agriculture field in Chhattisgarh region. The amounts of siderophore produce by P. fluorescens isolates were screen in iron deficient succinate media and most of them were found positive for the production of much siderophores. One of the isolate from Pakhanjore area "P3" produce highest siderophore, which further assessed for plant growth promotion activities of crops rice, urd, mung and arhar. The study showed significantly higher increase in root length over control plants was observed in Rice (68.45%) followed by Urd (40.1%), Mung (33.71%) and Arhar (29.53%). Similarly, significantly higher increase in shoot length over control plants was observed in Rice (68.925%) followed by Urd (43.42%), Mung (39.96%) and Arhar (19.45%). It has been shown that these bacteria competively colonize plant roots and cause the plant statistically significant root and shoot increases by stimulating plant growth and reduce the incidence of plant disease of above mention crops under green house of tray culture conditions. Highlights• There were 59 isolates of pseudomonas fluorescens isolated from rhizosphere and non-rhizospheric soil.• P. fluorescens isolates were screen in iron deficient succinate media for amount of siderophore production.• One of isolate highest siderophore producing further assessed for PGPA.• The study showed significantly higher increase in shoot length and root length by P. fluorescens

Temperature-induced modulation of stress-tolerant PGP genes bioprospected from Bacillus sp. IHBT-705 associated with saffron (Crocus sativus) rhizosphere: A natural -treasure trove of microbial biostimulants

“…Siderophore-mediated iron uptake in plants is a major benefit imparted by PGPRs (Braud et al, 2006;Mandal and Kotasthane, 2014). Siderophores are grouped into three principal categories based on their functional groups which are hydroxamates, catecholate, and carboxylates (Suarez et al, 2019;Kramer et al, 2020).…”

Plant growth-promoting rhizobacteria (PGPR) and its mechanisms against plant diseases for sustainable agriculture and better productivity

“…The provision of iron to plants through soil bacteria is more important when the plants are exposed to an environmental stress such as heavy metal pollution. In these cases, siderophores help to get rid of the stresses imposed on plants by high soil levels of heavy metals (Mandal and Kotasthane, 2014). The continuous use of fungicides leads to development of fungal resistant strains transforming fungicides ineffective.…”