Isolation and antimicrobial activity of the phytoalexin 6-methoxymellein from cultured carrot cells

Kurosaki, Fumiya; Nishi, Arasuke

doi:10.1016/s0031-9422(00)86959-9

Cited by 130 publications

(61 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Four replicate tubes were always prepared and incubated at26°C for 24 h. A set of replicates was then combined and cells were harvested by filtration. 6-Methoxymellein was extracted from the cells, partially purified, and developed on a TLC plate (Merck, silica gel 60 F 254) as described previously in detail (10). Concentration of the compound was determined by the scanning method at sample wavelength 265 nm on a dual wavelength chromatoscanner (Shi.…”

Section: Methodsmentioning

confidence: 99%

Breakdown of Phosphatidylinositol during the Elicitation of Phytoalexin Production in Cultured Carrot Cells

Kurosaki¹,

Tsurusawa²,

Nishi³

1987

Plant Physiol.

Self Cite

106

View full text Add to dashboard Cite

Elicitor-induced production of the phytoalexin, 6-methoxymellein, in cultured carrot cells was appreciably depressed by the calmodulin inhibitors N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide and trifluo-perazine. An inhibitor of Ca2-phospholipid dependent protein kinase (protein kinase C), 1-(5-lisquinolinesulfonyl)-2-methylpiperazine, also inhibited the phytoalexin production in carrot. Both phorbol ester and synthetic diacylglycerol, activators of protein kinase C, showed an ability to induce 6-methoxymellein production even in the absence of elicitor.Phosphatidylinositol-degrading phospholipase activity increased in elicitor-treated carrot cells without a notable lag, and a product of this reaction, inositol trisphosphate, appeared to increase in parallel with the phospholipase activity. These results suggest that breakdown of phosphatidylinositol takes place in the elicitor-treated carrot cells. The messengers liberated from the phospholipid in the plasma membrane may participate in the elicitation process by controlling the activity of protein kinase C-like enzyme(s) and Ca2@-inediated processes including calmodula.

show abstract

Section: Methodsmentioning

confidence: 99%

Breakdown of Phosphatidylinositol during the Elicitation of Phytoalexin Production in Cultured Carrot Cells

Kurosaki¹,

Tsurusawa²,

Nishi³

1987

Plant Physiol.

Self Cite

106

View full text Add to dashboard Cite

show abstract

“…The isolation of amicoumacins A (1) and B (2) [4ϳ6] and closely related AI-77s [7,8] from Bacillus pumilus evoked interest in medicinal research because of their characteristic biological activities including antibacterial, anti-inflammatory, antiulcer, gastroprotective, and anti-Helicobacter pylori activities [1ϳ3, 7,9,10]. Their unique basic structures ( Fig.…”

mentioning

confidence: 99%

Isolation of 8′-Phosphate Ester Derivatives of Amicoumacins: Structure-activity Relationship of Hydroxy Amino Acid Moiety

et al. 2007

View full text Add to dashboard Cite

Two new compounds, 8Ј-phospho derivatives of amicoumacins A and B, were isolated from the culture broth of a strain of Bacillus pumilus together with amicoumacins A and B. Their structures were elucidated on the basis of spectroscopic methods and alkaline phosphatase treatments. Comparison of the antibacterial activities against methicillin-resistant Staphylococcus aureus (MRSA) of these compounds suggested that C-8Ј hydroxyl and C-12Ј amide group of amicoumacin A played a critical role for anti-MRSA activity.

show abstract

“…globosum stimulated the release of heat-stable secondary elicitors from carrot cells. Since we have shown that a commercial pectinase elicits the production of phytoalexin in carrot cells (Kurosaki & Nishi, 1984), we did experiments to determine whether the heat-labile factor derived from the fungus was a pectinase or related enzyme.…”

Section: R E S U L T Smentioning

confidence: 99%

“…2) may be attributable to the extraction procedure, which involves pronase treatment. Pronase has been shown to release heat-stable elicitor from carrot cell homogenates (Kurosaki & Nishi, 1984). The difference in response of cultured carrot cells to the hydrolysates of carrot pectic fraction and citrus pectin may be partly related to differences in their chemical composition.…”

Section: A M I N F K U R O S a K I A N D A N I S H Imentioning

confidence: 99%

“…Lee & West ( I98 1 a, b) and Davis et al (1984) showed that pectinolytic enzymes released into the culture filtrates of Rhizopus stolonifer and Erwinia carotovora elicited phytoalexin accumulation in castor bean and soybean, respectively. Kurosaki & Nishi (1983 showed that carrot produces 6-methoxymellein as a common phytoalexin after being challenged by various fungi, and that wall fragments released from carrot cells by a commercial pectinase or protease elicit the production of the phytoalexin. These findings suggested that fungal pectinolytic enzymes play a role in the elicitation of phytoalexin production in carrot-fungi interactions.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Extracellular Pectinolytic Enzymes of Fungi Elicit Phytoalexin Accumulation in Carrot Suspension Culture

1986

View full text Add to dashboard Cite

A heat-labile elicitor of phytoalexin accumulation in carrot (Daucus curota L.) was detected in culture filtrate of Chaetomium globosum grown in Czapek-Dox medium. The elicitor activity correlated with the activity of pectinolytic enzymes in the culture filtrates. The culture filtrates induced the release of heat-stable elicitors from carrot cell homogenates, pectic fraction of carrot cell walls and citrus pectin. The coincidence of heat sensitivity and sodium periodate inactivation profiles of elicitor and pectinolytic activity suggested that the activity of pectinolytic enzymes is essential for the release of the heat-stable elicitor. The culture filtrates of Botrytis cinerea, Fusarium rnoniliforrne and Helrninthosporium oryzue showed similar elicitation patterns.

show abstract

Isolation and antimicrobial activity of the phytoalexin 6-methoxymellein from cultured carrot cells

Cited by 130 publications

References 16 publications

Breakdown of Phosphatidylinositol during the Elicitation of Phytoalexin Production in Cultured Carrot Cells

Breakdown of Phosphatidylinositol during the Elicitation of Phytoalexin Production in Cultured Carrot Cells

Isolation of 8′-Phosphate Ester Derivatives of Amicoumacins: Structure-activity Relationship of Hydroxy Amino Acid Moiety

Extracellular Pectinolytic Enzymes of Fungi Elicit Phytoalexin Accumulation in Carrot Suspension Culture

Contact Info

Product

Resources

About