Isolation and anticancer activity evaluation of rare Bisaryl anthraquinone antibiotics from novel Streptomyces sp. strain of NW Himalayan region

A biostatistical approach for augmenting rare bianthraquinone antibiotic production by Streptomyces sp. RA-WS2 using Taguchi design

“…Although few groups have reported the isolation and bioactivities of setomimycin from different Streptomyces strains (Präg et al, 2014 ; Omura et al, 1978 ), however could not be commercially exploited much due to its limited access in the market. Recently, our group has reported a potential Streptomyces strain for the production of setomimycin and its bioactivities (Manhas et al, 2022a , b ). Keeping in view the lower yields of production by the reported strains (Kay et al, 1981 ), attempts were made to develop an efficient fermentation process for the production of setomimycin.…”

“…The isolate has recently been reported for the production of a bianthraquinone antibiotic i.e. setomimycin under submerged fermentation for its antiviral and anticancer properties by Manhas and coworkers ( 2022a , b ).…”

“…Post fermentation, the fermented broth was homogenized with 10% methanol for about 2 h and thereafter extracted at least thrice with equal volumes of ethyl acetate as described previously (Manhas et al, 2022b ). The organic phase thus obtained was concentrated on a rotary evaporator at 50 °C to get crude extract.…”

“…Setomimycin is reported to be active against various Gram-positive bacteria such as B. subtilis, S. aureus, M. smegmatis and B. cereus. Recently, our group has also reported a potential strain for setomimycin production and its bioactive potential as antiviral as well as anticancer agent (Manhas et al, 2022a , b ).…”

“…In the present study, we have used an actinobacterium Streptomyces sp. strain RA-WS2 that has recently been identified for setomimycin production (Manhas et al, 2022a , b ). The culture parameters that could influence the production of setomimycin; such as carbon source, nitrogen source, agitation and aeration rate were optimized by methodological application of Taguchi design using an OA layout of L9.…”