Isolation and analysis of restriction endonuclease digestive patterns of chromosomal DNA from Mycobacterium paratuberculosis and other Mycobacterium species

Whipple, Diana L.; Febvre, R B Le; Andrews, Robert E.; Thiermann, A B

doi:10.1128/jcm.25.8.1511-1515.1987

Cited by 74 publications

(24 citation statements)

References 13 publications

(11 reference statements)

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“…Merkal's proposal was motivated by the atypical properties of St-18, including rapid and profuse growth, mycobactin production, and absence of pathogenicity for calves. Our study unambiguously shows identity between St 18 and the M. avium type strain and thus confirmed previous analyses of restriction fragment patterns by conventional electrophoresis and serotype-specific peptidoglycolipid characterization (4,16,28). Pulsedfield electrophoresis revealed that M. paratuberculosis strain 316 F (14) differed from the other M. paratuberculosis strains, A VOL.…”

Section: Discussionsupporting

confidence: 90%

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DNA polymorphism in Mycobacterium paratuberculosis, "wood pigeon mycobacteria," and related mycobacteria analyzed by field inversion gel electrophoresis

et al. 1989

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Mycobacterium paratuberculosis strains, mycobacteria from patients suffering from Crohn's disease, "wood pigeon mycobacteria," and representatives of Mycobacterium avium-Mycobacterium intracellulare were compared by restriction endonuclease DraI digestion and field inversion gel electrophoresis. Characteristic profiles were seen for M. paratuberculosis, including isolates from patients suffering from Crohn's disease, for wood pigeon mycobacteria, and for M. avium-M. intracelulare serotypes 2, 16, 18, and 19. Two M. paratuberculosis strains used for vaccine production (St 18 and 316 F) presented patterns different from those of the other M. paratuberculosis strains. Strains St 18 yielded a pattern identical to that of the M. avium type strain serotype 2, whereas 316 F gave a unique pattern. The method developed in this study represents a useful taxonomic tool for the identification and classification of mycobacteria.

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Section: Discussionsupporting

confidence: 90%

“…Restriction endonuclease digestion profiles of the DNA from M. avium and M. paratuberculosis strains showed stringent conservation of genetic composition among strains of M. paratuberculosis and high heterogeneity within M. avium species (9,27,28). However, these electrophoretic * Corresponding author.…”

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confidence: 99%

DNA polymorphism in Mycobacterium paratuberculosis, "wood pigeon mycobacteria," and related mycobacteria analyzed by field inversion gel electrophoresis

et al. 1989

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“…Paratuberculosis or Johne's disease (JD) is a chronic infectious disease that affects all categories of domestic and wild ruminants including cattle, sheep, goats, camels, buffaloes, and farmed deer (20,26,33,38). JD is known to occur worldwide and is responsible for important economic losses (8,41). The causative agent, Mycobacterium paratuberculosis, is a slowly growing, acid-fast microorganism that, unlike other cultivable mycobacteria, requires exogenous supplementation with ferric mycobactin J for growth in primary culture (8).…”

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confidence: 99%

Molecular characterization of Mycobacterium paratuberculosis isolates from sheep, goats, and cattle by hybridization with a DNA probe to insertion element IS900

et al. 1996

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Mycobactin J-dependent mycobacterial isolates from sheep, goat, and cattle herds with Johne's disease in Morocco, South Africa, the United States, and Germany were tested for the repetitive insertion sequence IS900 of Mycobacterium paratuberculosis by PCR. The IS900 PCR target sequence was detected in 90 of 93 fecal culture isolates tested (96.8%). Restriction fragment length polymorphisms (RFLPs) and in vitro growth characteristics were studied in 46 of the IS900-positive isolates and in two bovine vaccine strains of M. paratuberculosis. Five different RFLP types were identified in PvuII digests of genomic DNA by Southern hybridization with a DNA probe specific for IS900. All isolates of M. paratuberculosis could be classified into two major clusters by their growth rates as well as the relatedness of their PvuII-RFLP hybridization patterns. All of the sheep isolates were classified into cluster I (extremely slow growth), while all cattle and goat isolates were members of cluster II (moderately slow growth). Different PvuII-RFLP patterns were detected in different sheep flocks from Morocco and South Africa. Our results demonstrate that genetically and phenotypically different strains of M. paratuberculosis were present in ruminant populations. The strains from sheep in Morocco and South Africa tested in the study appeared to belong to a unique group of M. paratuberculosis strains that might have adapted to this host species. The presence of several genetically distinct strains in different sheep flocks suggested that analysis of IS900-specific RFLP patterns may provide a useful tool for the epidemiologic investigation of ovine paratuberculosis outbreaks. MATERIALS AND METHODSBacterial strains and growth conditions. A total of 93 mycobacterial field isolates was examined in the study. All of the strains had been cultured from fecal samples by standard procedures and were maintained aerobically at 37ЊC on Loewenstein-Jensen solid medium supplemented with mycobactin J (Rhone Merieux, Laupheim, Germany). Isolates were presumptively identified as M. paratuberculosis on the basis of slow growth, mycobactin J dependency, acid fastness (by Ziehl-Neelsen staining), and colony morphology (8). The strains obtained in Morocco were isolated during a survey of three farms located 85 km north (farms M1 and M2) and 15 km south (farm M3) of the city of Rabat. A frequent exchange of some animals between farms M1 and M2 was reported. On farm M1, 17 presumptive M. paratuberculosis strains were isolated from 51 sheep by fecal culture methods, 23 strains were isolated from 80 sheep on farm M2, and 14 strains were isolated from 49 sheep on farm M3. On farm M1, one strain was also occasionally isolated from a cow. All animals tested were suspected of suffering from paratuberculosis because they exhibited slow, progressive weight loss and in some cases had diarrhea nonresponsive to anthelmintic or antibiotic treatment. Isolation of M. paratuberculosis strains from sheep on farm M1 and corresponding clinical and histopathological finding...

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“…The upstream primer was constructed with a HindIll restriction site, and the downstream primer was constructed with a Sall restriction site. M. leprae chromosomal DNA was purified by a modification of methods described by Whipple et al (55). Template DNA consisted of ca.…”

Section: Methodsmentioning

confidence: 99%

Chemical definition, cloning, and expression of the major protein of the leprosy bacillus

et al. 1994

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The decline in prevalence of leprosy is not necessarily matched by a fall in incidence, emphasizing the need for new antigens to measure disease transmission and reservoirs of infection. Mycobacterium leprae obtained from armadillo tissues was disrupted and subjected to differential centrifugation to arrive at preparations of cell wall, cytoplasmic membrane, and cytosol. By committing 0.3 g of M. leprae to the task, it was possible to isolate from the cytosol and fully define the major cytosolic protein. Amino-terminus sequencing and chemical and enzymatic cleavage, followed by more sequencing and fast atom bombardment-mass spectrometry of fragments, allowed description of the entire amino acid sequence of a protein of 10,675-Da molecular mass. The sequence derived by chemical means is identical to that deduced previously from DNA analysis of the gene of a 10-kDa protein, a GroES analog. The work represents the first complete chemical definition of an M. leprae protein. PCR amplification of the 10-kDa protein gene, when cloned into Escherichia coli with a pTRP expression vector, allowed production of the recombinant protein. Chemical analysis of the expressed protein demonstrated that it exactly reflected the native protein. The recombinant major cytosolic protein appears to be a promising reagent for skin testing, still probably the most appropriate and pragmatic means of measuring incidence of leprosy.

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Isolation and analysis of restriction endonuclease digestive patterns of chromosomal DNA from Mycobacterium paratuberculosis and other Mycobacterium species

Cited by 74 publications

References 13 publications

DNA polymorphism in Mycobacterium paratuberculosis, "wood pigeon mycobacteria," and related mycobacteria analyzed by field inversion gel electrophoresis

DNA polymorphism in Mycobacterium paratuberculosis, "wood pigeon mycobacteria," and related mycobacteria analyzed by field inversion gel electrophoresis

Molecular characterization of Mycobacterium paratuberculosis isolates from sheep, goats, and cattle by hybridization with a DNA probe to insertion element IS900

Chemical definition, cloning, and expression of the major protein of the leprosy bacillus

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