Isolation and analysis of mannose/trehalose/maltose specific lectin from jack bean with antibruchid activity

Sakthivelkumar, Shanmugavel; Veeramani, Velayutham; Kamalanathan, Tamilarasan; Mullainadhan, P.; Arumugam, Muthu; Janarthanan, Sundaram

doi:10.1016/j.ijbiomac.2016.05.093

Cited by 9 publications

(5 citation statements)

References 56 publications

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“…Canavalia oxyphylla seed lectin was likewise reported to retain full activity until 60 °C, lost considerable activity at 70 °C and no activity was observed at 90 °C (Santiago et al, 2014). Lectin purified from Phaseolus coccineus (Pan and Ng, 2015), Chenopodium quinoa (Pompeu et al, 2015), Apuleia leiocarpa (Carvalho et al, 2015) and Canavalia virosa (Shanmugavel et al, 2016) are all thermostable up to 60 °C for at least 30 min. High thermostability possessed by these lectins may be advantageous, as stable bioactive substance is more efficient during all phases of their processing and on the other hand this is considered as antinutritional factors that cause many adverse phenomena in animals if ingested (e Lacerda et al, 2017).…”

Section: Resultsmentioning

confidence: 99%

“…the reported pH stability for lectin from Erythrina indica leaves lectin that exhibited pH stability at pH 6.0 -pH 8.0 (Konozy et al, 2002), A. hypogea seed which maintained its maximum activity at pH 5.0 to pH 8.0 (Sun et al, 2011), Dioclea lasiocarpa seeds lectin optimum pH was between 6.0 and 8.0 (do Nascimento et al, 2012) and C. virosa seeds lectin have highest activity at pH 4.0 -pH 9.0 (Shanmugavel et al, 2016).…”

Section: Resultsmentioning

confidence: 99%

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Purification, Characterization and Antioxidant Potential of a Novel Lectin from Pterocarpus soyauxii Taub Seeds

2019

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The study investigated the presence of lectin in the seeds of Pterocarpus soyauxii, purified the lectin and determined its physicochemical properties as well as its antioxidant potential. Purification of P. soyauxii seeds lectin (PSSL) was accomplished by ammonium sulphate precipitation and gel filtration on Sephadex G-100, hemagglutination assay was by serial dilution and DPPH radical scavenging, metal chelating and lipid peroxidation inhibition assays were employed to evaluate its antioxidant potential. PSSL agglutinated erythrocytes from human blood group ABO, rabbit and rat. Trypsinization enhanced agglutination which was inhibited by galactose, lactose and arabinose. The lectin activity was independent of metal ion, thermal stable up to 60 °C and at pH range of 6 to 8. Gel filtration estimated molecular weight was 65 kDa and subunit molecular weight was 35 kDa. PSSL showed significant antioxidant activity. The lectin was able to scavenge DPPH radical, chelate iron metal and inhibit lipid peroxidation with IC50 of 0.14 ± 1.33 mg/ml, 3.40 ± 0.37 mg/ml and 0.50 ± 0.08 mg/ml respectively. The results indicate that PSSL might be applied as natural antioxidant for the development of drug and food products for the benefit of human health.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Purification, Characterization and Antioxidant Potential of a Novel Lectin from Pterocarpus soyauxii Taub Seeds

2019

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“…Previous studies revealed that trehalose can remarkably reduce the mean day survival rate of sweet potato whitefly Bemisia tabaci (Gennadius) [ 29 ], and trehalose/mannose/maltose specific lectins exhibited promising insecticidal activities against stored product pests [ 30 ]. Azelaic acid was also detected at the active sites of a variety of plants with remarkable insecticidal activities [ 31 ].…”

Section: Resultsmentioning

confidence: 99%

Insecticidal Activities Against Odontotermes formosanus and Plutella xylostella and Corresponding Constituents of Tung Meal from Vernicia fordii

Zhang

Chen

Lü

et al. 2021

Insects

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The environmental pollution, pesticide resistance, and other associated problems caused by traditional chemical pesticides with limited modes of action make it urgent to seek alternative environmentally-friendly pesticides from natural products. Tung meal, the byproduct of the detoxified Vernicia fordii (Hemsl.) seed, has been commonly used as an agricultural fertilizer and as a pesticide. However, its active insecticidal extracts and ingredients remain elusive. In the present study, the contact toxicities of tung meal extracts against the agricultural and forest pests like O. formosanus and P. xylostella were examined. Our results showed that ethyl acetate and petroleum ether extracts showed the strongest toxicity against O. formosanus and P. xylostella, respectively. In order to further explore the chemical profiles of the ethyl acetate and petroleum ether extracts, UPLC-Q/TOF-MS and GC-MS analyses have been performed, and 20 and 29 compounds were identified from EA and PE extracts, respectively. The present study, for the first time, verified the noteworthy insecticidal activities on the aforementioned agricultural and forest pesticides and revealed the potential active parts and chemical composition, which are conducive to further exploiting the potential of tung meal as a natural plant-derived insecticide for biological control of agricultural and forest pests.

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“…The seed lectins of some legume species have been shown to be highly toxic to C . maculatus 25–27 . Because LecRK possesses a lectin domain, it may be toxic to C .…”

Section: Discussionmentioning

confidence: 99%

Development of an SNP-based high-density linkage map and QTL analysis for bruchid (Callosobruchus maculatus F.) resistance in black gram (Vigna mungo (L.) Hepper)

Somta

Chen

Yundaeng

et al. 2019

Sci Rep

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Black gram ( Vigna mungo var. mungo ) is an important pulse crop in Asia. The cowpea weevil ( Callosobruchus maculatus ) is a stored-seed insect pest (seed weevil/bruchid) that causes serious postharvest losses in pulse crops, including black gram. In this study, we constructed a high-density linkage map for black gram and identified quantitative trait loci (QTLs) for C . maculatus resistance. A recombinant inbred line (RIL) population of 150 lines from a cross between BC48 [cultivated black gram (var. mungo ); bruchid-susceptible] and TC2210 [wild black gram (var. silvestris ); bruchid-resistant] were used to construct a linkage map of 3,675 SNP markers from specific-locus amplified fragment sequencing. The map comprised 11 linkage groups spanning 1,588.7 cM with an average distance between adjacent markers of 0.57 cM. Seeds of the RIL population grown in 2016 and 2017 were evaluated for C . maculatus resistance through two traits; the percentage of damaged seeds (PDS) and infestation severity progress (AUDPS). Inclusive composite interval mapping identified three QTLs each for PDS and AUDPS. Two QTLs, qVmunBr6 .1 and qVmunBr6 .2, mapped about 10 cM apart on linkage group 6 were common between PDS and AUDPS. Comparative genome analysis revealed that qVmunBr6 . 1 and qVmunBr6 . 2 are new loci for C . maculatus resistance in Vigna species and that genes encoding a lectin receptor kinase and chitinase are candidates for qVmunBr6 . 2 . The high-density linkage map constructed and QTLs for bruchid resistance identified in this study will be useful for molecular breeding of black gram.

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Isolation and analysis of mannose/trehalose/maltose specific lectin from jack bean with antibruchid activity

Cited by 9 publications

References 56 publications

Purification, Characterization and Antioxidant Potential of a Novel Lectin from Pterocarpus soyauxii Taub Seeds

Purification, Characterization and Antioxidant Potential of a Novel Lectin from Pterocarpus soyauxii Taub Seeds

Insecticidal Activities Against Odontotermes formosanus and Plutella xylostella and Corresponding Constituents of Tung Meal from Vernicia fordii

Development of an SNP-based high-density linkage map and QTL analysis for bruchid (Callosobruchus maculatus F.) resistance in black gram (Vigna mungo (L.) Hepper)

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