Isolation and analyses of uranium tolerant Serratia marcescens strains and their utilization for aerobic uranium U(VI) bioadsorption

Kumar, Rakshak; Acharya, Celin; Joshi, S. R.

doi:10.1007/s12275-011-0366-0

Cited by 57 publications

(29 citation statements)

References 23 publications

(20 reference statements)

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“…These kinds of media are composed of complex, chemically undefined substances which precipitate metals and make them unavailable for bacteria, thus resulting in higher EC 50 and MIC values Rathnayake et al, 2013). Moreover, higher EC 50 and MIC values can also be observed in solid media in comparison to liquid media (Kumar et al, 2011;Yilmaz, 2003). Growth experiments in the present study show that the tolerance to Hg is almost ten times higher in the NB compared to LP (Figs.…”

Section: Effect Of Mercury On Growth Of Strain Sa2mentioning

confidence: 49%

Bioremediation potential of a highly mercury resistant bacterial strain Sphingobium SA2 isolated from contaminated soil

et al. 2016

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A mercury resistant bacterial strain, SA2, was isolated from soil contaminated with mercury. The 16S rRNA gene sequence of this isolate showed 99% sequence similarity to the genera Sphingobium and Sphingomonas of α-proteobacteria group. However, the isolate formed a distinct phyletic line with the genus Sphingobium suggesting the strain belongs to Sphingobium sp. Toxicity studies indicated resistance to high levels of mercury with estimated EC50 values 4.5 mg L(-1) and 44.15 mg L(-1) and MIC values 5.1 mg L(-1) and 48.48 mg L(-1) in minimal and rich media, respectively. The strain SA2 was able to volatilize mercury by producing mercuric reductase enzyme which makes it potential candidate for remediating mercury. ICP-QQQ-MS analysis of Hg supplemented culture solutions confirmed that almost 79% mercury in the culture suspension was volatilized in 6 h. A very small amount of mercury was observed to accumulate in cell pellets which was also evident according to ESEM-EDX analysis. The mercuric reductase gene merA was amplified and sequenced. The deduced amino acid sequence demonstrated sequence homology with α-proteobacteria and Ascomycota group.

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Section: Effect Of Mercury On Growth Of Strain Sa2mentioning

confidence: 49%

Bioremediation potential of a highly mercury resistant bacterial strain Sphingobium SA2 isolated from contaminated soil

et al. 2016

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“…The second approach involves any type of manipulated or stimulated or enhanced biological remediation of an environment (Hazen 2010). The introduction of specific microorganisms to the contaminated site accelerates the degradation process by generating or enhancing conducive physicochemical conditions (Kumar et al 2011b). When site conditions are not suitable, engineered bioremediations are introduced to the particular site, especially using genetically engineered bacteria.…”

Section: In Situ Bioremediationmentioning

confidence: 99%

“…Solid phase treatment includes organic wastes (leaves, manures and agricultural wastes) and problematic wastes, for example domestic/industrial wastes and sewage sludge/ municipal solid wastes. The treatment processes include soil biopiles, composting, land farming and hydroponics (Ramos et al 2011;Kumar et al 2011b;Rayu et al 2012). A biopile is a short-term bioremediation technology in which excavated soils are mixed with soil amendments, formed into compost piles or cells aboveground and enclosed for treatment supported with aeration system (Li et al 2004).…”

Section: Ex Situ Bioremediationmentioning

confidence: 99%

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Biotechnological advances in bioremediation of heavy metals contaminated ecosystems: an overview with special reference to phytoremediation

Mani

Kumar

2013

Int. J. Environ. Sci. Technol.

507

137

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The ability of heavy metals bioaccumulation to cause toxicity in biological systems-human, animals, microorganisms and plants-is an important issue for environmental health and safety. Recent biotechnological approaches for bioremediation include biomineralization (mineral synthesis by living organisms or biomaterials), biosorption (dead microbial and renewable agricultural biomass), phytostabilization (immobilization in plant roots), hyperaccumulation (exceptional metal concentration in plant shoots), dendroremediation (growing trees in polluted soils), biostimulation (stimulating living microbial population), rhizoremediation (plant and microbe), mycoremediation (stimulating living fungi/mycelial ultrafiltration), cyanoremediation (stimulating algal mass for remediation) and genoremediation (stimulating gene for remediation process). The adequate restoration of the environment requires cooperation, integration and assimilation of such biotechnological advances along with traditional and ethical wisdom to unravel the mystery of nature in the emerging field of bioremediation. This review highlights better understanding of the problems associated with the toxicity of heavy metals to the contaminated ecosystems and their viable, sustainable and eco-friendly bioremediation technologies, especially the mechanisms of phytoremediation of heavy metals along with some case studies in India and abroad. However, the challenges (biosafety assessment and genetic pollution) involved in adopting the new initiatives for cleaning-up the heavy metals-contaminated ecosystems from both ecological and greener point of view must not be ignored.

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“…A total of 155 selected isolates were subjected to 16S rDNA sequence analysis following the methods as described by Kumar et al (2011). Briefly, Genomic DNA was extracted using Genomic DNA isolation kit (HiPurA Bacterial and Yeast DNA Purification Spin Kit, HiMedia, India).…”

Section: Molecular Characterizationmentioning

confidence: 99%

Cultivable bacterial diversity along the altitudinal zonation and vegetation range of tropical Eastern Himalaya

Lyngwi

Koijam

Sharma

et al. 2013

RBT

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Abstract:The Northeastern part of India sprawls over an area of 262 379km 2 in the Eastern Himalayan range. This constitutes a biodiversity hotspot with high levels of biodiversity and endemism; unfortunately, is also a poorly known area, especially on its microbial diversity. In this study, we assessed cultivable soil bacterial diversity and distribution from lowlands to highlands (34 to 3 990m.a.s.l.). Soil physico-chemical parameters and forest types across the different altitudes were characterized and correlated with bacterial distribution and diversity. Microbes from the soil samples were grown in Nutrient, Muller Hinton and Luria-Bertani agar plates and were initially characterized using biochemical methods. Parameters like dehydrogenase and urease activities, temperature, moisture content, pH, carbon content, bulk density of the sampled soil were measured for each site. Representative isolates were also subjected to 16S rDNA sequence analysis. A total of 155 cultivable bacterial isolates were characterized which were analyzed for richness, evenness and diversity indices. The tropical and sub-tropical forests supported higher bacterial diversity compared to temperate pine, temperate conifer, and sub-alpine rhododendron forests. The 16S rRNA phylogenetic analysis revealed that Firmicutes was the most common group followed by Proteobacteria and Bacteroidetes. Species belonging to the genera Bacillus and Pseudomonas were the most abundant. Bacterial CFU showed positive but insignificant correlation with soil parameters like pH (r=0.208), soil temperature (r=0.303), ambient temperature (r=0.443), soil carbon content (r=0.525), soil bulk density (r=0.268), soil urease (r=0.549) and soil dehydrogenase (r=0.492). Altitude (r=-0.561) and soil moisture content (r=-0.051) showed negative correlation. Altitudinal gradient along with the vegetation and soil physico-chemical parameters were found to influence bacterial diversity and distribution. This study points out that this is a biome with a vast reservoir of bacteria which decrease with increasing altitudes, and highlights the microbiological importance of the poorly studied Eastern Himalayan range, justifying efforts to explore the prevalence of novel species in the biome. Rev. Biol. Trop. 61 (1): 467-490. Epub 2013 March 01.

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Isolation and analyses of uranium tolerant Serratia marcescens strains and their utilization for aerobic uranium U(VI) bioadsorption

Cited by 57 publications

References 23 publications

Bioremediation potential of a highly mercury resistant bacterial strain Sphingobium SA2 isolated from contaminated soil

Bioremediation potential of a highly mercury resistant bacterial strain Sphingobium SA2 isolated from contaminated soil

Biotechnological advances in bioremediation of heavy metals contaminated ecosystems: an overview with special reference to phytoremediation

Cultivable bacterial diversity along the altitudinal zonation and vegetation range of tropical Eastern Himalaya

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