Isolation and Abiotic Stress Resistance Analyses of a Catalase Gene fromIpomoea batatas(L.) Lam

Abstract

As an indicator of the antioxidant capability of plants, catalase can detoxify reactive oxygen species (ROS) generated by environmental stresses. Sweet potato is one of the top six most important crops in the world. However, its catalases remain largely unknown. In this study, a catalase encoding gene, IbCAT2 (accession number: KY615708), was identified and cloned from sweet potato cv. Xushu 18. It contained a 1479 nucleotides' open reading frame (ORF). S-R-L, Q-K-L, and a putative calmodulin binding domain were located at the C-terminus of IbCAT2, which suggests that IbCAT2 could be a peroxisomal catalase. Next-generation sequencing (NGS) based quantitative analyses showed that IbCAT2 was mainly expressed in young leaves and expanding tuberous roots under normal conditions. When exposed to 10% PEG6000 or 200 mmol/L NaCl solutions, IbCAT2 was upregulated rapidly in the first 11 days and then downregulated, although different tissues showed different degree of change. Overexpression of IbCAT2 conferred salt and drought tolerance in Escherichia coli and Saccharomyces cerevisiae. The positive response of IbCAT2 to abiotic stresses suggested that IbCAT2 might play an important role in stress responses.

RNA-sequencing analysis revealed genes associated drought stress responses of different durations in hexaploid sweet potato

“…These genes have a major role in detoxification of ROS in the plant cell, which is extremely harmful as it causes lipid oxidation, DNA damage, and apoptosis. In agreement with our study, the role of protein detoxification has been documented in many plant species, i.e., Ipomoea batatas 18,88 , Ipomoea pes-caprae 89 , Arabidopsis thaliana 90 , Nicotiana tabacum 91 , Oryza sativa L. 92 and Solanum lycopersicum 93 . In addition, TSA genes are involved in the detoxification of reactive oxygen species (ROS), reactive sulfur species (RSS), and reactive nitrogen species (RNS).…”

Impact of Drought Stress during Germination on Antioxidant Capacities and Antioxidant Enzymes Activities of Madura Local Maize (<i>Zea mays</i>) Seeds

“…CAT gene expression is also affected by the stress condition, and it significantly increased after 2 days. A Similar result in Ipomea batatas was reported by literature [30] CAT gene expression is increased significantly after 2 days and decreased on the 20 days. The same finding is observed in other plants such as Pitaya [36], rice [37], Medicago falcet, and Cleome spinosa.…”

“…Stress affects the expression of the APX gene in the early stage of germination (11 days). After 6 weeks, it has no significant effect [29] [30]. In literature [31] was reported that some APX gene is increased under stress condition on rice.…”

Transcriptome analysis of phosphorus stress responsiveness in the seedlings of Dongxiang wild rice (Oryza rufipogon Griff.)

“…And another one gene encoded homeobox domain containing protein ( LOC_Os11g06020.1 ), whose products are transcription factors sharing a characteristic protein fold structure that bound DNA [ 63 – 65 ], regulating gene transcription. Furthermore, another gene, LOC_Os11g44310.1 , which encoded calmodulin binding protein, regulated the expression of calmodulin to regulate the intracellular calcium concentration to control cells in many important biochemical reactions, which might play an important role in stress responses [ 66 ].…”