Isolation &amp; characterization of Brucella melitensis isolated from patients suspected for human brucellosis in India

Barua, Anita; Kumar, Adarsh; Thavaselvam, Duraipandian; Mangalgi, Smita S; Prakash, Avinash; Tiwari, Sapana; Arora, Sonia; Sathyaseelan, Kannusamy

doi:10.4103/0971-5916.187115

Cited by 26 publications

(14 citation statements)

References 24 publications

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“…Most misdiagnosed patients were admitted in Department of Rheumatology, Hematology, Orthopedics, and Respiration at the first visit. Brucella bacteria culture is the “gold standard” for the diagnosis of brucellosis [ 95 , 96 ]. In the study, 87% of brucellosis patients have fever.…”

Section: Discussionmentioning

confidence: 99%

A Systematic Review and Meta-Analysis of Epidemiology and Clinical Manifestations of Human Brucellosis in China

Zheng

Xie

et al. 2018

BioMed Research International

157

126

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Background. Brucellosis has a wide spectrum of clinical manifestations and it may last several days or even several years; however, it is often misdiagnosed and therefore may cause inadequate therapy and prolonged illness. Previous studies about meta-analysis of manifestations of brucellosis reported in English lacked the data published in Chinese, which did not provide details about the contact history, laboratory tests, and misdiagnosis. We undertake a meta-analysis of clinical manifestations of human brucellosis in China to identify those gaps in the literature. We have searched published articles in electronic databases up to December 2016 identified as relating to clinical features of human brucellosis in China. 68 studies were included in the analysis. The main clinical manifestations were fever, fatigue, arthralgia, and muscle pain (87%, 63%, 62%, and 56%, resp.). There are significant differences between adults and children. Rash, respiratory and cardiac complications, and orchitis/epididymitis were more prevalent in children patients. The common complications of brucellosis were hepatitis, followed by osteoarthritis, respiratory diseases, cardiovascular diseases, central nervous system dysfunction, hemophagocytic syndrome, and orchitis/epididymitis in male. In the nonpastoral areas, brucellosis has a high ratio of misdiagnosis. Our analysis provides further evidence for the accurate diagnosis, particularly in assessing severe, debilitating sequelae of this infection.

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Section: Discussionmentioning

confidence: 99%

A Systematic Review and Meta-Analysis of Epidemiology and Clinical Manifestations of Human Brucellosis in China

Zheng

Xie

et al. 2018

BioMed Research International

157

126

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“…Naturally, the Castañeda method is not specific for brucellae, and other microorganisms, including true pathogens and contaminants, may grow in the flask; therefore, full identification of the isolate is required. Although the performance of the Castañeda method has been superseded in recent years by automated blood culture systems, it is still extensively employed in countries of endemicity with limited economic and technical resources due to its low cost and practicality (27,(42)(43)(44)(45)(46)(47).…”

Section: Table 1 Factors That Determine the Positivity Rate Of Blood mentioning

confidence: 99%

Laboratory Diagnosis of Human Brucellosis

2019

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SUMMARY The clinical presentation of brucellosis in humans is variable and unspecific, and thus, laboratory corroboration of the diagnosis is essential for the patient’s proper treatment. The diagnosis of brucellar infections can be made by culture, serological tests, and nucleic acid amplification assays. Modern automated blood culture systems enable detection of acute cases of brucellosis within the routine 5- to 7-day incubation protocol employed in clinical microbiology laboratories, although a longer incubation and performance of blind subcultures may be needed for protracted cases. Serological tests, though they lack specificity and provide results that may be difficult to interpret in individuals repeatedly exposed to Brucella organisms, nevertheless remain a diagnostic cornerstone in resource-poor countries. Nucleic acid amplification assays combine exquisite sensitivity, specificity, and safety and enable rapid diagnosis of the disease. However, long-term persistence of positive molecular test results in patients that have apparently fully recovered is common and has unclear clinical significance and therapeutic implications. Therefore, as long as there are no sufficiently validated commercial tests or studies that demonstrate an adequate interlaboratory reproducibility of the different homemade PCR assays, cultures and serological methods will remain the primary tools for the diagnosis and posttherapeutic follow-up of human brucellosis.

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“…melitensis [ 53 ]. Several studies have demonstrated that the 16S rRNA can be used as a rapid tool for Brucella identification [ 53 , 54 ]. In this study, we identified 38 Brucella isolates with PCR by targeting the 16S rRNA gene in the first round of screening and these were further identified as B .…”

Section: Discussionmentioning

confidence: 99%

Abortion and various associated risk factors in dairy cow and sheep in Ili, China

et al. 2020

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We studied livestock abortion and various associated risk factors in the Ili region of northwest China. Livestock abortion prevalence was estimated and correlated with infections (Brucellosis, Salmonellosis, Mycoplasma and Chlamydia seropositivity) and management (farming type and contact with other herds/flocks) risk factors. A total of 2996 serum samples (1406 cow, 1590 sheep) were identified by RBPT (Rose Bengal Plate Test) and c-ELISA (competitive-enzyme linked immunosorbent assay), and they showed the overall seroprevalence of brucellosis in the study area was cow 6.76%, sheep 9.50%. The seroprevalence of brucellosis in X county was cow 7.06%, sheep 9.12%; in H county was cow 11.70%, sheep 10.80%; and in Q county was cow 4.22%, sheep 9.11%. The overall seroprevalence of Mycoplasma in the study area was cow 3.20%, sheep 6.42%. The seroprevalence of Mycoplasma in X county was cow 3.39%, sheep 7.98%; in H county was cow 5.26%, sheep 9.97%; and in Q county was cow 2.11%, sheep 4.33%. The Odds ratio of brucellosis for cow and sheep, respectively, were 45.909 [95% CI 26.912–78.317, P <0.001] and 70.507 [95% CI 43.783–113.544, P <0.001] times higher than other abortion-related factors including mixed farming, contact with other flocks and Mycoplasma infection. A total of 54 samples, including aborted cow (22), sheep (30) fetuses and milk samples (2), were identified as Brucella melitensis ( B . melitensis ) positive. A total of 38 Brucella were isolated from 16 aborted cow, 20 sheep fetuses and 2 milk samples. All of these isolates were identified, and confirmed, as B . melitensis . A phylogenetic tree showed that the Brucella isolates closely matched the B . melitensis biovar 3 isolated in Inner Mongolia, China, and B . melitensis isolated from Norway and India. These results suggest that B . melitensis biovar 3 is the main pathogen responsible for cow and sheep abortion and also pose a human health risk. Additionally, livestock reproduction can also be influenced by Mycoplasma infection and managerial factors (farming type and contact with other herds/flocks), especially in remote areas.

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Isolation & characterization of Brucella melitensis isolated from patients suspected for human brucellosis in India

Cited by 26 publications

References 24 publications

A Systematic Review and Meta-Analysis of Epidemiology and Clinical Manifestations of Human Brucellosis in China

A Systematic Review and Meta-Analysis of Epidemiology and Clinical Manifestations of Human Brucellosis in China

Laboratory Diagnosis of Human Brucellosis

Abortion and various associated risk factors in dairy cow and sheep in Ili, China

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