Isolating the metabolic pathways involved in the hepatoprotective effect of Muntingia calabura against CCl4-induced liver injury using LC/MS Q-TOF

Rofiee, Mohd Salleh; Yusof, Mat Ikram; Hisam, Elly Ezlinda Abdul; Bannur, Zakaria; Zunita, Z.; Somchit, M. N.; Teh, Lay Kek; Salleh, Mohd Zaki

doi:10.1016/j.jep.2015.03.016

Cited by 30 publications

(19 citation statements)

References 28 publications

(26 reference statements)

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“…Thus, we emphasized the comparisons between EA/LPS group and the other extracts/LPS groups to find potential metabolomic biomarkers associated with idiosyncratic hepatotoxicity. Reports have shown that metabolomic approach based on the UHPLC-MS can be used as a robust tool to inspect the effects of hepatotoxic compounds (Weiss and Kim, 2011; Xue et al, 2014; Rofiee et al, 2015). Accordingly, UHPLC-MS based metabolomic technique was utilized used to investigate the alterations of plasma metabolite profiles in rats after combined exposure to LPS and with different extracts of Heshouwu.…”

Section: Discussionmentioning

confidence: 99%

“…Because this is a noninvasive technique, many diagnostic biomarkers and pathways of the organism can be tracked prior to toxicant exposure or during disease. Metabolomics has been successfully employed to identify specific biomarkers of toxicant exposure, which eventually could be used for early warnings of liver injury in clinic (Weiss and Kim, 2011; Xue et al, 2014; Rofiee et al, 2015). …”

Section: Introductionmentioning

confidence: 99%

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Metabolomic Study on Idiosyncratic Liver Injury Induced by Different Extracts of Polygonum multiflorum in Rats Integrated with Pattern Recognition and Enriched Pathways Analysis

Gao

et al. 2016

Front. Pharmacol.

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Currently, numerous liver injury cases related to a famous Chinese herb- Polygonum Multiflorum (Heshouwu in Chinese) have attracted great attention in many countries. Our previous work showed that Heshouwu-induced hepatotoxicity belonged to idiosyncratic drug-induced liver injury (IDILI). Unfortunately, the components and mechanisms attributed to IDILI of Heshouwu are difficult to determine and thus remain unknown. Attempts to explore puzzles, we prepared the chloroform (CH)-, ethyl acetate (EA)-, and residue (RE) extracts of Heshouwu to investigate IDILI constituents and underlying mechanisms, using biochemistry, histopathology, and metabolomics examinations. The results showed that co-treatment with non-toxic dose of lipopolysaccharide (LPS) and EA extract could result in evident liver injury, indicated by the significant elevation of plasma alanine aminotransferase and aspartate aminotransferase activities, as well as obvious liver histologic damage; whereas other two separated fractions, CH and RE extracts, failed to induce observable liver injury. Furthermore, 21 potential metabolomic biomarkers that differentially expressed in LPS/EA group compared with other groups without liver injury were identified by untargeted metabolomics, mainly involved two pathways: tricarboxylic acid cycle and sphingolipid metabolism. This work illustrated EA extract had close association with the idiosyncratic hepatotoxicity of Heshouwu and provided a metabolomic insight into IDILI of different extracts from Heshouwu.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Metabolomic Study on Idiosyncratic Liver Injury Induced by Different Extracts of Polygonum multiflorum in Rats Integrated with Pattern Recognition and Enriched Pathways Analysis

Gao

et al. 2016

Front. Pharmacol.

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“…It has been reported that free radicals or reactive oxygen species are the main factors responsible for liver injury (Lin, Dan, et al, 2019;Lu, Wang, Zhang, Liu, & Xu, 2017). Through lipid peroxidation of biological membranes, they cause damage to liver cell membranes, mitochondrial membranes and other biological membranes, resulting in degeneration and necrosis of liver cells, and an increase of lipid peroxides in the body (Kakuni et al, 2012;Rofiee et al, 2015). MDA is the product of lipid peroxidation.…”

Section: Discussionmentioning

confidence: 99%

Analysis of Streptococcus agalactiae ‐induced liver injury in tilapia ( Oreochromis niloticus )

Cao

Jia

et al. 2020

Aquac Res

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Streptococcosis, a bacterial disease for multiple fish species, especially tilapia, caused more disruption to the fish culture industry. However, the underlying mechanisms for the occurrence of streptococcosis remain unclear. The aim of this study was to study the effects of streptococcus on the liver of tilapia. In this study, tilapia were injected streptococcal solution (0.05 ml/10 g body weight) and collected blood and liver at 4, 8, 24, 48, 72, 96, 120 and 144 hr after injection. The results showed that the activities of glutamate oxalate transaminase (GOT), glutamate pyruvate transaminase (GPT), alkaline phosphatase (AKP) and superoxide dismutase (SOD) in the serum of tilapia exhibited irregular changes: increase at first, then decrease and increase again. The levels of total antioxidant capacity (T‐AOC), glutathione (GSH) and malondialdehyde (MDA) showed a decreasing tendency in serum after injection, except GSH at 4 hr. The activity of catalase (CAT) decreased in serum at 4, 24, 120 and 144 hr after injection of streptococcal solution, while the content TP enhanced in serum at 48, 72 and 120 hr after injection. Observations of pathological sections showed obvious damage to the liver tissue structure in response to streptococcal infection. Western blotting revealed an increase in ikkbeta (Ikkβ) protein with prolonged infection time and a decrease in NF‐kappa‐B inhibitor alpha (IkB‐α) and ikappab kinase alpha (Ikkα) proteins. The gene expression of cytochrome P4501A (CYP1A) was downregulated in liver at 24 and 48 after injection, while the mRNA levels of heat stress protein 70 (HSP70) and tumour necrosis factor alpha (TNF‐α) increased at 48 and/or 72 hr. Taken together, our findings confirmed that streptococcus can cause serious damage to the liver of tilapia.

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“…Oleh karena itu, tanaman yang mengandung flavonoid banyak dimanfaatkan sebagai bahan obat herbal. Selain itu, beberapa penelitian telah melaporkan efek farmakologi dari M. calabura sebagai antioksidan (Buhian et al, 2017;Balan et al, 2015;Zakaria et al, 2014a;Sindhe et al, 2013;Gomathi et al, 2013;Preethi et al, 2012;Zakaria et al, 2011;Siddiqua et al, 2010;Preethi et al, 2010); anti-inflamasi (Balan et al, 2015;Zakaria et al, 2014a;Gomathi et al, 2013;Preethi et al, 2012;Zakaria et al, 2007a); antimikroba (Buhian et al, 2016;Rajesh et al, 2014;Sufian et al, 2013;Sibi et al, 2013;Sibi et al, 2012); antidiabetes (Khan et al, 2015;Sindhe et al, 2013;Sridhar et al, 2011); antikanker (Rofiee et al, 2015;Sufian et al, 2013;Chen et al, 2004;Chen et al, 2005;Kaneda et al, 1991); antinyeri (Zakaria et al, 2016;Zakaria et al, 2014b;Yusof et al, 2013;Sani et al, 2012;Zakaria et al, 2007b); antiulcer (Balan et al, 2015;Ibrahim et al, 2012) dan lainlain. Berdasarkan bioaktivitas M. calabura dari tinjauan tersebut, review ini bertujuan untuk memberikan fakta ilmiah terkait sinergitas senyawa metabolit sekunder dan aktivitas biologis dari M. calabura sebagai bahan acuan pada penelitian selanjutnya.…”

Section: Pendahuluanunclassified

Metabolit sekunder dari Muntingia calabura dan bioaktivitasnya

Putri

Fatmawati

2019

ALCHEMY J.Pen.Kim

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Muntingia calabura (Muntingiaceae) merupakan Jamaican cherry yang dikenal di Indonesia sebagai Kersen atau Talok. Metabolit sekunder sebagai konstituen kimia telah diisolasi dari daun, batang dan akar M. calabura. Flavonoid merupakan konstituen utama penyusun metabolit sekunder dari tanaman ini. Kelompok flavonoid telah dilaporkan memiliki efek farmakologi yang baik. Beberapa literatur melaporkan bioaktivitas M. calabura sebagai antioksidan, antidiabetes, antimikroba, antikanker, anti-inflamasi dan lain-lain. Review ini bertujuan memberikan fakta ilmiah terkait sinergitas metabolit sekunder dan bioaktivitas M. calabura yang diperlukan untuk penelitian kimia bahan alam lebih lanjut.The secondary metabolites of Muntingia calabura and its bioactivity. Muntingia calabura (Mutingiaceae) was recognized as Jamaican cherry called as Kersen or Talok in Indonesia. The chemical constituents have been isolated from leave, stem and root of M. calabura. The main chemical constituent of the secondary metabolite is flavonoid. The flavonoid group has been reported as a good source in pharmacological aspect. Most of literatures reported that M. calabura has a good bioactivity as an antioxidant, antidiabetic, antimicrobial, anticancer, anti-inflammatory and others. This review aims to provide the scientific evidences related to the synergism of secondary metabolites and the bioactivities of M. calabura for further research on natural products.

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Isolating the metabolic pathways involved in the hepatoprotective effect of Muntingia calabura against CCl4-induced liver injury using LC/MS Q-TOF

Cited by 30 publications

References 28 publications

Metabolomic Study on Idiosyncratic Liver Injury Induced by Different Extracts of Polygonum multiflorum in Rats Integrated with Pattern Recognition and Enriched Pathways Analysis

Metabolomic Study on Idiosyncratic Liver Injury Induced by Different Extracts of Polygonum multiflorum in Rats Integrated with Pattern Recognition and Enriched Pathways Analysis

Analysis of Streptococcus agalactiae ‐induced liver injury in tilapia ( Oreochromis niloticus )

Metabolit sekunder dari Muntingia calabura dan bioaktivitasnya

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