Isolating highly enriched populations of circulating epithelial cells and other rare cells from blood using a magnetic sweeper device

Talasaz, AmirAli; Powell, Ashley A.; Huber, David; Berbee, James G.; Roh, Kyung Ho; Yu, Wong; Xiao, Wenzhong; Davis, Mark M.; Pease, R. F. W.; Mindrinos, Michael; Jeffrey, Stefanie S.; Davis, Ronald W.

doi:10.1073/pnas.0813188106

Cited by 437 publications

(310 citation statements)

References 24 publications

Supporting

Mentioning

306

Contrasting

Unclassified

Order By: Relevance

“…We demonstrated that our MDA-MB-231 xenograft model shed CTCs into mouse circulation using a new technology that enabled viable human CTC capture from mouse blood (Talasaz et al, 2009). Magnetic beads attached to the epithelial cell adhesion molecule antigen on CTCs appeared as dark clusters of beads on the cell membrane surrounding translucent cells.…”

Section: Distinctive Expression Of Anoxia-induced Factors In Hypoxic mentioning

confidence: 99%

“…Magnetically labelled blood samples were transferred to a six-well plate and brought up to a final volume of 10 ml using PBS. The magnetically labelled samples were then processed by the newly developed MagSweeper that isolates CTCs by multiple rounds of magnetic capture, wash, and release as previously described (Talasaz et al, 2009). Briefly, magnetic capturing rods covered by plastic sheaths were robotically swept through wells containing magnetically labelled mouse blood.…”

Section: Isolation Of Circulating Tumour Cellsmentioning

confidence: 99%

See 1 more Smart Citation

Circulating tumour cells demonstrate an altered response to hypoxia and an aggressive phenotype

et al. 2010

View full text Add to dashboard Cite

BACKGROUND: Tumours contain hypoxic regions that select for an aggressive cell phenotype; tumour hypoxia induces metastasisassociated genes. Treatment refractory patients with metastatic cancer show increased numbers of circulating tumour cells (CTCs), which are also associated with disease progression. The aim of this study was to examine the as yet unknown relationship between hypoxia and CTCs. METHODS: We generated human MDA-MB-231 orthotopic xenografts and, using a new technology, isolated viable human CTCs from murine blood. The CTCs and parental MDA-MB-231 cells were incubated at 21 and 0.2% (hypoxia) oxygen, respectively. Colony formation was assayed and levels of hypoxia-and anoxia-inducible factors were measured. Xenografts generated from CTCs and parental cells were compared. RESULTS: MDA-MB-231 xenografts used to generate CTCs were hypoxic, expressing hypoxia factors: hypoxia-inducible factor1 alpha (HIF1a) and glucose transporter protein type 1 (GLUT1), and anoxia-induced factors: activating transcription factor 3 and 4 (ATF3 and ATF4). Parental MDA-MB-231 cells induced ATF3 in hypoxia, whereas CTCs expressed it constitutively. Asparagine synthetase (ASNS) expression was also higher in CTCs. Hypoxia induced ATF4 and the HIF1a target gene apelin in CTCs, but not in parental cells. Hypoxia induced lower levels of carbonic anhydrase IX (CAIX), GLUT1 and BCL2/adenovirus E1B 19-KD protein-interacting protein 3 (BNIP3) proteins in CTCs than in parental cells, supporting an altered hypoxia response. In chronic hypoxia, CTCs demonstrated greater colony formation than parental cells. Xenografts generated from CTCs were larger and heavier, and metastasised faster than MDA-MB-231 xenografts. CONCLUSION: CTCs show an altered hypoxia response and an enhanced aggressive phenotype in vitro and in vivo.

show abstract

Section: Distinctive Expression Of Anoxia-induced Factors In Hypoxic mentioning

confidence: 99%

Section: Isolation Of Circulating Tumour Cellsmentioning

confidence: 99%

Circulating tumour cells demonstrate an altered response to hypoxia and an aggressive phenotype

et al. 2010

View full text Add to dashboard Cite

show abstract

“…Magnetic field-affected flow While pressure and electrokinetic manipulation of fluidic flows in microchannels are usually referred to as label-free techniques, magnetophoretic isolation of species belongs to the so-called labeled methods [53]. Magnetophoretic techniques, also referred to as magnetic-activated sorting methods, have been thoroughly described for cells utilizing adhesion-based microfluidic cell-sorting devices in a recent review [54].…”

Section: Electric Field-affected Flowsmentioning

confidence: 99%

Modeling of cell sorting and rare cell capture with microfabricated biodevices

Járvás

Guttman

2013

Trends in Biotechnology

View full text Add to dashboard Cite

“…Over the last years, there has been a rapid development of technologies for isolating DTCs, employing enrichment methods for further characterization of these cells. The enumeration and characterization of CTCs in PB can be done by different devices using EpCAM coating as antibody in the CellSearch system [59], microfluid devices (CTC-chip) [60], or MagSweeper [61]. In addition, the latest techniques generate microvortices to significantly increase the number of interactions between CTCs and the antibody-coated chip surface.…”

Section: Predictive Biomarkersmentioning

confidence: 99%

Radiotherapy and inhibition of the EGF family as treatment strategies for prostate cancer: combining theragnostics with theragates

2010

View full text Add to dashboard Cite

Isolating highly enriched populations of circulating epithelial cells and other rare cells from blood using a magnetic sweeper device

Cited by 437 publications

References 24 publications

Circulating tumour cells demonstrate an altered response to hypoxia and an aggressive phenotype

Circulating tumour cells demonstrate an altered response to hypoxia and an aggressive phenotype

Modeling of cell sorting and rare cell capture with microfabricated biodevices

Radiotherapy and inhibition of the EGF family as treatment strategies for prostate cancer: combining theragnostics with theragates

Contact Info

Product

Resources

About