Isolating and evaluating lactic acid bacteria strains for effectiveness of Leymus chinensis silage fermentation

Abstract

Five LAB strains were evaluated using the acid production ability test, morphological observation, Gram staining, physiological, biochemical and acid tolerance tests. All five strains (LP1, LP2, LP3, LC1 and LC2) grew at pH 4·0, and LP1 grew at 15°C. Strains LP1, LP2 and LP3 were identified as Lactobacillus plantarum, whereas LC1 and LC2 were classified as Lactobacillus casei by sequencing 16S rDNA. The five isolated strains and two commercial inoculants (PS and CL) were added to native grass and Leymus chinensis (Trin.) Tzvel. for ensiling. All five isolated strains decreased the pH and ammonia nitrogen content, increased the lactic acid content and LP1, LP2 and LP3 increased the acetic content and lactic/acetic acid ratio of L. chinensis silage significantly. The five isolated strains and two commercial inoculants decreased the butyric acid content of the native grass silage. LP2 treatment had lower butyric acid content and ammonia nitrogen content than the other treatments. The five isolated strains improved the quality of L. chinensis silage. The five isolated strains and the two commercial inoculants were not effective in improving the fermentation quality of the native grass silage, but LP2 performed better comparatively. Significance and impact of the study: Leymus chinensis is an important grass in China and Russia, being the primary grass of the short grassland 'steppe' regions of central Asia. However, it has been difficult to make high-quality silage of this species because of low concentration of water-soluble carbohydrates (WSC). Isolating and evaluating lactic acid bacteria strains will be helpful for improving the silage quality of this extensively grown species.

Effects of moisture content and additives on the ensiling quality and vitamins changes of alfalfa silage with or without rain damage

“…The pH and lactic acid were significantly affected by the LP + sucrose, which is consistent with previous studies (Filya, Muck, & Contreras-Govea, 2007;Muck et al, 2018;Wang, Wang, Zhou, & Feng, 2009). The AN/TN from this study was apparently reduced after supplementation of LP + sucrose, which was similar to the results of Zhang et al (2014) by using LP. The rain damage reduced the DM content of the silage in this study.…”

“…The concentrations of CP, neutral detergent fiber (NDF), acid detergent fiber (ADF), and WSC were 202, 351, 309, and 63.9 g/kg on a DM basis, respectively. The information of the L. plantarum (LP) was described previously, which was screened from grass silage by our Lab (Zhang, Li, Zhao, & Yu, 2014). Its nucleotide sequence for the 16S ribosomal DNA was deposited with GenBank under accession numbers: KR107070.…”

Fermentation Characteristics of Lactobacillus Plantarum and Pediococcus Species Isolated from Sweet Sorghum Silage and Their Application as Silage Inoculants

“…Yuan 2015, also found high LA when applied Lactobacillus spp in total mixed rations among other chemicals [44]. As the previous studies mentioned, the addition of LAB inoculants at ensiling is intended to ensure rapid and vigorous fermentation [45].…”

Effects of Lactic Acid Bacteria Isolated From Rumen Fluid and Feces of Dairy Cows on Fermentation Quality, Microbial Community, and in vitro Digestibility of Alfalfa Silage

“…The LAB strains with fast growth capacity and high acid-producing ability were tested for their physiological and biochemical characteristics. And 16S rRNA gene sequencing was employed to identify the screened strains genetically (Zhang et al, 2014).…”