Isolated heart perfusion according to Langendorff—Still viable in the new millennium

Skrzypiec-Spring, Monika; Grotthus, Bartosz; Szeląg, Adam; Schulz, Richard

doi:10.1016/j.vascn.2006.05.006

Cited by 272 publications

(196 citation statements)

References 57 publications

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“…The isolated Langendorff perfused heart remains a prominent tool for studying cardiac physiology 2 . It is especially useful in studies of cardiac arrhythmias, particularly those that use fluorescence imaging of transmembrane potential 20 .…”

Section: Discussionmentioning

confidence: 99%

NADH Fluorescence Imaging of Isolated Biventricular Working Rabbit Hearts

Asfour

Wengrowski

Jaimes

et al. 2012

JoVE

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Since its inception by Langendorff 1 , the isolated perfused heart remains a prominent tool for studying cardiac physiology 2 . However, it is not well-suited for studies of cardiac metabolism, which require the heart to perform work within the context of physiologic preload and afterload pressures. Neely introduced modifications to the Langendorff technique to establish appropriate left ventricular (LV) preload and afterload pressures 3 . The model is known as the isolated LV working heart model and has been used extensively to study LV performance and metabolism [4][5][6] . This model, however, does not provide a properly loaded right ventricle (RV). Demmy et al. first reported a biventricular model as a modification of the LV working heart model 7,8 . They found that stroke volume, cardiac output, and pressure development improved in hearts converted from working LV mode to biventricular working mode 8 . A properly loaded RV also diminishes abnormal pressure gradients across the septum to improve septal function. Biventricular working hearts have been shown to maintain aortic output, pulmonary flow, mean aortic pressure, heart rate, and myocardial ATP levels for up to 3 hours 8 .When studying the metabolic effects of myocardial injury, such as ischemia, it is often necessary to identify the location of the affected tissue. This can be done by imaging the fluorescence of NADH (the reduced form of nicotinamide adenine dinucleotide) [9][10][11] , a coenzyme found in large quantities in the mitochondria. NADH fluorescence (fNADH) displays a near linearly inverse relationship with local oxygen concentration 12 and provides a measure of mitochondrial redox state 13. fNADH imaging during hypoxic and ischemic conditions has been used as a dye-free method to identify hypoxic regions 14,15 and to monitor the progression of hypoxic conditions over time 10.The objective of the method is to monitor the mitochondrial redox state of biventricular working hearts during protocols that alter the rate of myocyte metabolism or induce hypoxia or create a combination of the two. Hearts from New Zealand white rabbits were connected to a biventricular working heart system (Hugo Sachs Elektronik) and perfused with modified Krebs-Henseleit solution 16 at 37 °C. Aortic, LV, pulmonary artery, and left & right atrial pressures were recorded. Electrical activity was measured using a monophasic action potential electrode. To image fNADH, light from a mercury lamp was filtered (350±25 nm) and used to illuminate the epicardium. Emitted light was filtered (460±20 nm) and imaged using a CCD camera. Changes in the epicardial fNADH of biventricular working hearts during different pacing rates are presented. The combination of the heart model and fNADH imaging provides a new and valuable experimental tool for studying acute cardiac pathologies within the context of realistic physiological conditions. Video LinkThe video component of this article can be found at https://www. 10.0 glucose, 2.00 NaPyruvate, and 20.0 mg/L albumin). The soluti...

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Section: Discussionmentioning

confidence: 99%

NADH Fluorescence Imaging of Isolated Biventricular Working Rabbit Hearts

Asfour

Wengrowski

Jaimes

et al. 2012

JoVE

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“…This vital technique is still widely used by a variety of cardiovascular researchers. Because the preparation is free of other organs, it allows the complete exclusion of peripheral influences on the isolated heart and the direct evaluation of heart function [22]. The analysis showed a slight elevation of LVPSP and a slight depression of LVEDP in the heart of DPZ rats, resulting in a significantly greater LVDP in the DPZ group than in the untreated group ( Figure 1 and Table 1).…”

Section: Discussionmentioning

confidence: 98%

Donepezil, Therapeutic Acetylcholinesterase Inhibitor, Prevents the Progression of Ventricular Dysfunction by Promoting Myocardial Glucose Utilization in Rat Model of Chronic Heart Failure Following Myocardial Infarction

Arikawa¹,

Kakinuma²,

Nakatani³

et al. 2014

Cardiol Pharmacol

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“…Modelling inflammatory responses The mucosal irritation potential of Kietzmann et al, 1993;Lugol's (Bäumer et al, 2002) chemical and pharmacological parameters and is generally accepted as a model to study drug-induced cardiotoxicity and ECG conductivity (Skrzypiec-Spring et al, 2007;Bell et al, 2011). The isolated heart can also be perfused in the working heart mode, i.e., the perfusate enters the left atrium via the pulmonary vein, flows into the left ventricle and onwards into the aorta (Chinchoy et al, 2000;Araki et al, 2005).…”

Section: Uterus (Bovine)mentioning

confidence: 99%

Extracorporeal perfusion of isolated organs of large animals – Bridging the gap between in vitro and in vivo studies

Daniel¹

2018

ALTEX

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Isolated heart perfusion according to Langendorff—Still viable in the new millennium

Cited by 272 publications

References 57 publications

NADH Fluorescence Imaging of Isolated Biventricular Working Rabbit Hearts

NADH Fluorescence Imaging of Isolated Biventricular Working Rabbit Hearts

Donepezil, Therapeutic Acetylcholinesterase Inhibitor, Prevents the Progression of Ventricular Dysfunction by Promoting Myocardial Glucose Utilization in Rat Model of Chronic Heart Failure Following Myocardial Infarction

Extracorporeal perfusion of isolated organs of large animals – Bridging the gap between in vitro and in vivo studies

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