Isolated 13ql4 Abnormalities and Normal Karyotypes Are Associated With Typical Lymphocyte Morphology in B-Cell Chronic Lymphocytic Leukemia

Abstract

Peripheral blood lymphocyte morphology and karyotype were correlated across the spectrum of cytogenetic abnormalities in 78 previously karyotyped cases of B-cell chronic lymphocytic leukemia (CLL). Cases were classified according to FrenchAmerican-British morphologic criteria as typical CLL or CLL, mixed-cell type; the latter category was divided into CLL with a mixture of small and large cells and CLL with increased prolymphocytes (CLL/PL). Other leukemic lymphoproliferative disorders were excluded from this analysis. CLL cases with normal karyotypes were more likely to demonstrate typical morphology than those with clonal abnormalities (P=.042). In addition, all six cases containing isolated 13ql4 abnormalities had typical morphology, compared with six of 16 other isolated abnormalities (P=.009), including one of seven cases of isolated trisomy 12. In contrast with the cases of isolated 13ql4 changes, only seven of 17 cases with 13ql4 as part of complex abnormalities had typical morphology (P=.012). Trisomy 12 was associated with mixed-cell morphology, particularly CLL/PL, consistent with previous reports. We conclude that isolated 13ql4 abnormalities and normal karyotype are associated with typical CLL morphology, while other clonal abnormalities, including trisomy 12, are associated with mixed-cell morphology. These results further support the concept of d i s t i n c t CLL s u b g r o u p s b a s e d on k a r y o t y p e . Furthermore, the association of trisomy 12 and complex abnormalities with mixed-cell morphology may have implications for clonal evolution in CLL.

Pan B‐cell markers are not redundant in analysis of chronic lymphocytic leukemia (CLL)

“…This finding was somewhat unexpected in light of several studies that have shown an apparent correlation between morphology and immunophenotype (65)(66)(67)(68)(69)(70)(71). In addition, CLL with trisomy 12 has been associated with atypical morphology (65)(66)(67)(68)(69)(70)(71).…”

“…This finding was somewhat unexpected in light of several studies that have shown an apparent correlation between morphology and immunophenotype (65)(66)(67)(68)(69)(70)(71). In addition, CLL with trisomy 12 has been associated with atypical morphology (65)(66)(67)(68)(69)(70)(71). Furthermore, cases of CLL with trisomy 12 have been shown to be associated with an atypical immunophenotype including increased CD20 expression (66,70) and expression of FMC7 (65,68,70,71).…”

Cyclin D1 overexpression allows identification of an aggressive subset of leukemic lymphoproliferative disorder

“…Most of the Correspondence: F Ajchenbaum-Cymbalista; Fax: 33 1 42 34 82 54 Received 7 January 1998; accepted 20 April 1999 studies conducted on atypical B-CLL have been devoted to their immunologic characteristics 2,3 or to cytogenetic abnormalities, [4][5][6][7][8][9][10][11] but clinical presentation and outcome have not been extensively studied, although they are considered as sharing a poorer prognosis than typical CLL. 12 Among the recurrent genetic alterations observed in B-CLD, the translocation t (11;14) has been recently highlighted, since it is the hallmark of MCL.…”

Secondary Abnormalities of Chromosome 6q in B-Cell Chronic Lymphocytic Leukemia: A Sequential Study of Karyotypic Instability in 51 Patients

“…When single cytogenetic abnormalities are examined, trisomy 12 is associated with shortened survival, whereas isolated abnormalities of chromosome 13q14 are associated with favorable outcomes, comparable to those of patients lacking clonal chromosome abnormalities [2]. Previous studies suggest that the morphologic and immunophenotypic variation observed in B-CLL is also related to karyotype [3][4][5][6][7].…”