ISOLASI DAN UJI AKTIVITAS ANTIBAKTERI DARI BAKTERI YANG BERASOSIASI DENGAN ALGA Turbinaria ornata (Turner) J. Agardh SERTA IDENTIFIKASI SECARA BIOKIMIA

Abstract

Algae are a place to live for various microorganisms’ symbiosis with them, and some of them were known to be used as antibacterial. This study aims to determine the antibacterial activity of associated bacteria isolates from Turbinaria ornata algae obtained from the Bay of Manado against Staphylococcus aureus and Escherichia coli bacteria. In the course of testing the isolation and purification of symbiotic bacteria from Turbinaria ornata algae, then antibacterial activity was testing using agar diffusion method, then gram staining and biochemical tests were carried out including motility test, tets, carbohydrate fermentation test, citrate test, lysine test, indole test and catalase test. The result showed that there were 3 bacterial isolates, which had antibacterial activity in medium category against S.aureus and E.coli bacteria. Based on the results of biochemical identification, the isolates that have the greatest inhibitory power, they were thought to belong to genus Bacillus and the genus Yersinia.Keywords : Antibacterial, Symbionic bacteria, Turbinaria ornata (Turner) J. Agardh. ABSTRAK Alga merupakan tempat hidup berbagai mikroorganisme yang bersimbiosis dengannya, dan beberapa dari spesies alga diketahui sering dimanfaatkan salah satunya sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui adanya aktivitas antibakteri dari isolat bakteri yang berasosiasi dengan alga Turbinaria ornata yang diperoleh dari Teluk Manado terhadap bakteri Staphylococcus aureus dan Escherichia coli. Dalam pengujian dilakukan isolasi dan purfifikasi bakteri simbion dari alga Turbinari ornata, kemudian dilakukan pengujian aktivitas antibakteri menggunakan metode difusi agar, selanjutnya silakukan pewarnaan gram dan uji biokimia meliputi uji motilitas, uji H2S, uji fermentasi karbohidrat, uji sitrat, uji lisin, uji indol dan uji katalase. Hasil penelitian menunjukkan bahwa terdapat 3 isolat bakteri yang mempunyai daya aktivitas antibakteri dalam kategori sedang terhadap bakteri S. aureus dan E. coli. Berdasarkan hasil identifikasi secara biokimia isolat yang mempunyai daya hambat paling besar diduga termasuk dalam genus Bacillus dan genus Yersinia. Kata kunci : Antibakteri, Bakteri simbion, Turbinaria ornata (Turner) J. Agardh.

Case report; Identification of Bacteria in Catfish (Pangasius sp.) at the Palembang Fish Quarantine Station

“…The formation of H2S is marked by the appearance of black color on the butt. This is in accordance with the research results of Kosasi et al (2019) positive H2S test results indicate the presence of black deposits at the bottom of the media used. In the TSIA test, if the H2S results produce a dark black color so that you cannot see the results of the color change in the pierced media, then the way to anticipate this is by checking periodically after incubation for 18 hours or more.…”

Inhibition of Lactobacillus acidophillus, Lactobacillus, Bacteria plantarum and Bifidobacterium spp. against Escherichia coli and Salmonella sp. in the digestive tract in vitro

“…The media that has become solid will then be inoculated with bacterial isolates aseptically using a loop needle inserted in the middle until it reaches 3/4 of the surface of the media. The ose needle that was inserted will be scratched on the inclined plane of the media and will then be incubated for 1x24 hours at 37°C [9].…”

“…is facultative, Bifidobacterium is anaerobic, L. acidophillus and L. plantarum are known to not have bacterial growth. The fermentation test is carried out to determine the ability of a bacterium to ferment sugar to produce acid or gas [9]. The results of the tests carried out are listed in table 1 This research was conducted with the aim of knowing the ability of probiotics such as Lactobacillus acidophilus, Lactoacilus plantarum, and Bifidobacterium sp.…”

Isolasi Bakteri Fiksasi Nitrogen dan Fosfat pada Limbah Cair Karet (Hevea brasiliensis)

“…Isolasi bakteri limbah cair karet dilakukan tes uji biokimia yang terdiri dari katalase, sitrat, motolitas, dan TSIA. Hasil uji katalase pada isolat limbah cair karet BS1, BS2, BS3, BS4, BS6, BS7, BS8, BS9, dan BS10 menunjukkan reaksi positif yang ditandai dengan terbentuk gelembung oksigen pada permukaan koloni setelah ditetesi dengan H2O2 menunjukkan bahwa organisme menghasilkan enzim katalase yang mengubah hydrogen peroksida menjadi air dan oksigen (Kosasi et al, 2019).…”