Isolasi dan Identifikasi Senyawa Flavonoid pada Tanaman

Ichsani, Audi; Lubis, Christina Febiola; Urbaningrum, Lestari Mahardika; Rahmawati, Nurma Dwi; Anggraini, Sridevi

doi:10.46799/jhs.v2i6.188

Cited by 5 publications

(4 citation statements)

References 2 publications

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“…After that the plant extract was dripped with a 10% NaOH solution. If the color of plant extracts changes from red to brown, the sample is declared positive for phenol group flavonoids [57].…”

Section: Flavonoids Testmentioning

confidence: 99%

Biological potential of eight medicinal plants collected in the restored landscape after mining in South Kalimantan

Wiraswati,

Pradini,

Fauziah

et al. 2024

Discov Appl Sci

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Land restoration is expected to enhance the supply of valuable ecosystem resources such as herbaceous bushes and weeds. This study aimed to determine the primary phytochemical constituents and bioactivities of methanol extracts from eight bushes and weeds collected from a restored post-mining landscape in South Kalimantan, Indonesia. Qualitative phytochemical analysis showed the presence of phenolic compounds, flavonoids, tannins, alkaloids, steroids, terpenoids and saponins in the methanol extracts of herbaceous plants. Their antioxidant activity was measured by using the 2,2-diphenyl-1-picryl-hydrazyl-hydrate assay. Their superoxide dismutase (SOD) activity was also measured. In addition, selected plant extracts were screened against the common human pathogens Staphylococcus aureus and Candida albicans. Phytochemical analysis showed that the methanol extracts contained all the bioactive compounds examined in this study except the one from Lycopodium cernuum, which lacked flavonoids and alkaloids. Further investigation revealed that all methanol extracts except the one from L. cernuum had promising antioxidant potential. The methanol extracts from Chromolaena odorata (stem), Trema micrantha, Melastoma malabathricum (flower and leaf) and Thypa angustifolia exhibited effective antibacterial activity. In addition, the methanol extracts from M. malabathricum (flower and leaf), T. micrantha, Scleria sumatrensis and Breynia cernua (leaf) exhibited effective antifungal activity. M. malabathricum (flower and leaf) has the greatest potential as a herbaceous plant since its methanol extract exhibits the most potent antioxidant, antibacterial and antifungal activities.

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Section: Flavonoids Testmentioning

confidence: 99%

Biological potential of eight medicinal plants collected in the restored landscape after mining in South Kalimantan

Wiraswati,

Pradini,

Fauziah

et al. 2024

Discov Appl Sci

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“…Pengujian flavonoid dengan pereksi Mg(s)+HCl(p), FeCl 3 5%, NaOH10% memberikan hasil positif karena terbentuknya warna orange-jingga yang menandakan adanya flavonoid (Aribowo et al, 2021;Nikmah et al, 2022;Pratama et al, 2023). Pengujian tanin dengan pereaksi FeCl 3 1% memberikan hasil positif yang ditandai dengan perubahan warna hijau kehitaman yang menandakan posistif adanya senyawa tanin (Nikmah et al, 2022).…”

Section: Hasil Pembuatan Ekstrak Daun Salam (Syzygium Polyanthum)unclassified

SKRINING DAN UJI AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL DAUN SALAM (Syzygium polyanthum (Wight) Walp) DENGAN METODE FRAP (Ferric Reducing Antioxidant Power)

Tambunan,

Nadia,

Siregar

2024

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Tanaman dari keluarga Myrtaceae yaitu daun salam yang memiliki nama taksonomi syzygium polyanthum (Wight) Walp memiliki banyak metabolit turunannya yang bisa menurunkan kolestrol dan hipertensi sehingga tanaman ini dijadikan tanaman obat di Indonesia. Senyawa flavonoid yang menjadi bagian dari metabolit turunan dalam tanaman ini yang dijadikan sebagai antioksidan alami. Penelitian ini merupakan metode deskriptif dan eksperimental. Proses maserasi dipakai untuk mendapatkan ekstrak kental dari daun salam dengan pelarut etanol 96%. Dilakukan pemeriksaan fitokimia dari hasil ekstraksi yang bertujuan untuk memastikan secara pasti metabolit turunan yang terdapat dalam daun salam. Metode FRAP dipilih sebagai pendekatan untuk mengukur kapasitas antioksidan di dalam daun salam. Rendemen dari proses ekstraksi dari daun salam dengan pelarut etanol 96% diperoleh sebanyak 6,3%. Pada pemeriksiaan fitokimia dari ekstraksi dari daun salam mendapatkan hasil positif yaitu saponin, flavonoid, tanin, glikosida, alkaloid dan steroid/triterpenoid. Pendekatan dengan menggunakan metode FRAP dilakukan 3 kali pengulangan. Nilai yang didapat dari masing-masing replikasi yaitu replikasi 1 (83,66 mgAAE/g ekstrak), replikasi 2 (71,58 mgAAE/g ekstrak), replikasi 3 (83,36 mgAAE/g ekstrak) dan mendapatkan nilai rata-rata dari ke 3 replikasi yaitu 79,53±6,88.

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“…Furthermore, the red color formed is caused by benzopyrylium salt. The reduction of polyhydroxy groups from flavanols produces a red color due to the existence of the Mg element in HCl [33].…”

Section: Phytochemical Test and Functional Groupsmentioning

confidence: 99%

The activity of Pometia pinnata leaf extract against pathogenic bacteria in fish

Setiaji,

Febian Prokoso,

Heriyanto

et al. 2024

BIO Web Conf.

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The use of antibiotics to cure bacterial diseases has a negative impact on fish and the environment, so it is necessary to look for natural products that are safe for treating fish diseases. This study aims to determine the activity of P. pinnata extract as an antibacterial towards V. alginolyticus, P. aeruginosa, A. salmonicida, E. ictaluri, A. hydrophila, and E. tarda bacteria. P. pinnata extract was obtained by maceration using ethanol solvent. This extract was tested for its phytochemical content, characterized by FT-IR and tested for its antibacterial activity by the agar diffusion method. The results of the phytochemical test of P. pinnata extract produced terpenoids compounds flavonoids, saponins and phenolic. The results of the FT-IR characterization of P. pinnata extract contained O-H, C-H, C=O and C-O groups. The results of the inhibition test showed that P. pinnata extract was able to inhibit the growth of V. alginolyticus bacteria by 13.0 mm to 14.7 mm. P. aeruginosa 14.5 mm to 17.1 mm. A. salmonicida by 12.4 mm to 14.9 mm. E. ictaluri by 13.5 mm to 15.5 mm. A. hydrophila 13.9 mm to 17.1 mm. E. tarda 13.6 mm to 15.5 mm. In conclusion, P. pinnata extract can be used to inhibit the growth of pathogenic bacteria in fish.

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Isolasi dan Identifikasi Senyawa Flavonoid pada Tanaman

Cited by 5 publications

References 2 publications

Biological potential of eight medicinal plants collected in the restored landscape after mining in South Kalimantan

Biological potential of eight medicinal plants collected in the restored landscape after mining in South Kalimantan

SKRINING DAN UJI AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL DAUN SALAM (Syzygium polyanthum (Wight) Walp) DENGAN METODE FRAP (Ferric Reducing Antioxidant Power)

The activity of Pometia pinnata leaf extract against pathogenic bacteria in fish

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