Isolasi dan Identifikasi Morfologi Jamur Selulolitik dari Limbah Rumah Tangga di Desa Sanur Kauh, Bali

Sutari, Ni Wayan Sri

doi:10.21107/agrovigor.v13i2.7443

Cited by 5 publications

(7 citation statements)

References 3 publications

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“…To calculate the phosphate solubility index, the sum of the colony diameter and clear zone diameter was divided by the colony diameter [26]. Similarly, the cellulolytic index was calculated by subtracting the colony diameter from the clear zone diameter and then dividing the result by the colony diameter [27].…”

Section: Testing the Potential Of Endophytic Fungi As Phosphate Solub...mentioning

confidence: 99%

“…The value of the cellulolytic which is less than 1 indicates that all isolates fall under the criteria of being low in decompose cellulose. According Sutari [27] the criteria for the cellulolytic index values are as follows: less than 1 (including low criteria), between 1and 2 (moderate), greater than 2 (high), if does not form a clear zone, it means there is no cellulolytic activity. In addition to measuring the cellulolytic index, the potential of endophytic fungi as cellulose decomposer can be done by calculating the cellulase enzyme produced.…”

Section: Testing the Potential Of Endophytic Fungi As Phosphate Solub...mentioning

confidence: 99%

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Styrax sumatranus J.J.Sm endophytes fungi and its potential as phosphate solubilizing and cellulose decomposition

Elfiati,

Susilowati,

Manalu

et al. 2024

IOP Conf. Ser.: Earth Environ. Sci.

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Styrax sumatranus J.J.Sm is a plant from North Sumatra, which is widely used by community for religious rituals, medicines and it has economic value. Endophytic fungi are fungi that harbour inside the plant tissue without causing any negative effects to their host plant. Endophytic fungi isolated from the leaves and bark of Styrax sumatranus J.J.Sm. The aim of this research was to obtain endophytic fungi that have potential as phosphate solubilizing and cellulose decomposition. The bark and leaves of Styrax sumatranus J.J.Sm as a source of endophytic fungi were taken from Pandumaan village, Pollung District, Humbang Hasundutan Regency, North Sumatra. Test the potential of endophytic fungal as phosphate solubilizers using selective Pikovskaya media and as cellulose decomposition using carboxyl methyl cellulose (CMC) media plus congo red 0.1%. Then calculate the phosphate solubility index and cellulolytic index. The result show that, of 20 isolates tested, there were 12 isolates that had potential as phosphate solubilizers and 12 isolates had potential as cellulose decomposition. Phosphate solubility index dan cellulolytic index obtained range from 2.10-2.44 and 0.22-0.55 respectively.

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Section: Testing the Potential Of Endophytic Fungi As Phosphate Solub...mentioning

confidence: 99%

Section: Testing the Potential Of Endophytic Fungi As Phosphate Solub...mentioning

confidence: 99%

Styrax sumatranus J.J.Sm endophytes fungi and its potential as phosphate solubilizing and cellulose decomposition

Elfiati,

Susilowati,

Manalu

et al. 2024

IOP Conf. Ser.: Earth Environ. Sci.

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“…Rumus indeks selulolitik yaitu : Indeks Selulolitik = Sumber. (Puspawati et al, 2018) Keterangan : DB : Diameter zona bening (mm) DK : Diameter koloni (mm) Karakterisasi isolat fungi selulolitik meliputi pengamatan morfologi koloni fungi : pengamatan secara makroskopis yaitu dengan melihat warna koloni, permukaan koloni, dan bentuk koloni (Sutari, 2020), dan pengamatan secara mikroskopis dilakukan dengan dengan mengambil isolat secara tipis dan ditaruh di atas kaca benda yang diberi warna Lactofenol caton blue (Sepriyani, 2018). Kemudian diamati bentuk hifa, bentuk konidia, dan bentuk konidiofor.…”

Section: Bahan Dan Metodeunclassified

“…Dilihat dari pertumbuhannya bentuk koloni dari fungi Aspergillus sp tidak beraturan. Aspergillus sp memiliki daya selulolitik yang cukup tinggi sehingga mampu mendegradasi serat kasar pada limbah padat terutama pada serasah daun mangrove (Sutari, 2020).…”

Section: Hasil Dan Pembahasanunclassified

“…Hasil uji degradasi selulosa pada fungi selulolitik dari sampah rumah tangga didapat 44 isolat fungi selulolitik dengan genus Aspergillus sp, dan Trichoderma sp. Aspergillus sp merupakan salah satu jenis fungi eukariotik yang memiliki ciri-ciri hifa bersepta dan bercabang, konidia berbentuk rantai bewarna hijau (Sutari, 2020) Berdasarkan hasil pengukuran diameter zona bening dan pengukuran diameter koloni fungi selulolitik dari 10 isolatfungi selulolitik diperoleh seperti yang dapat dilihat pada tabel 3 didapatkan 3 isolat fungi selulolitik mempunyai nilai indeks selulolitik tertinggi dengan kode isolat FS8,FS9,FS3. Daya degradasi selulosa diklasifikasikan berdasarkan nilai indeks selulolitik dengan kategori rendah apabila nilai IS sedangkan apabila nilai IS antara 1 sampai dengan 2 atau maka masuk dalam kategori tinggi.…”

Section: Hasil Dan Pembahasanunclassified

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Karakterisasi Fungi Selulotik Dari Serasah Daun Mangrove Asal Hutan Mangrove Lamnga Kabupaten Aceh Besar

Syafrina Sari Lubis,

Marzha Faradilla,

Diannita Harahap

2022

kenanga

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Desa Lamnga Kabupaten Aceh Besar merupakan salah satu desa yang terletak di pesisir pantai dan merupakan kawasan hutan mangrove. Serasah daun mangrove merupakan penyumbang utama biomassa selulosa di lingkungan pesisir laut yang dibantu oleh mikroorganisme. Tujuan dari penelitian ini adalah untuk mengetahui karakteristik jamur selulolitik dari serasah daun mangrove dan mengetahui potensi enzim selulolitik yang dihasilkan oleh jamur selulolitik. Pengambilan sampel dilakukan secara acak dengan titik pengambilan berdasarkan lokasi ditemukannya serasah daun mangrove. Sampel serasah daun dimasukkan ke dalam media CMC dan diinkubasi selama 7 hari pada suhu 25-300C. Didapatkan empat jenis jamur selulolitik yaitu genus Aspergillus sp, Aspergillus niger, Penicillium, dan Culvularia sp. Hasil uji kemampuan degradasi selulosa diperoleh nilai indeks ≥11 , kategori sedang dengan kode isolat FS3, FS8, FS9, dan nilai indeks ≤11 , kategori lemah dengan kode isolat FS1, FS2, FS4, FS5, FS6 , FS7 dan FS10.

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Morphological identification of phosphate solubilizing and cellulolytic fungi from mangrove soil under Rhizophora stylosa stands

Elfiati

Delvian

Susilowati

et al. 2021

IOP Conf. Ser.: Earth Environ. Sci.

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Mangrove forest is one of the forest ecosystems that can be found in tidal areas. Mangrove forest have ecological, socio-economic benefits for organisms that live in the sea. One of the microorganism that live in mangrove forest is fungus. Therefore, this study aimed to isolate and identify phosphate solubilizing and cellulolytic fungi from mangrove soil. Soil samples were collected from a stands of Rhizophora stylosa in Lubuk Kertang Village, West Brandan District, Langkat Regency, North Sumatra. Soil samples were taken randomly with a dept of 0-20 cm. Isolation of phosphate solubilizing fungi was carried out using Pikovskaya medium and cellulolytic fungi using carboxyl methyl cellulose (CMC) medium. To determine the effectiveness of fungi qualitatively, the phosphate solubility and cellulolytic index were calculated. All isolates obtained were identified morphologically by observing colonies macroscopically and microscopically. This study obtained 12 isolates of phosphate solubilizing and 8 isolates of cellulolytic fungi. Phosphate solubility index ranging from 2.06-2.87 and cellulolytic index of 0.33-3.00. Morphological identifications showed that all isolates of phosphate solubilizing fungi belonged to the genus Aspergillus, while all isolates of cellulolytic fungi belonged to the genus Chaetomium.

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Isolasi dan Identifikasi Morfologi Jamur Selulolitik dari Limbah Rumah Tangga di Desa Sanur Kauh, Bali

Cited by 5 publications

References 3 publications

Styrax sumatranus J.J.Sm endophytes fungi and its potential as phosphate solubilizing and cellulose decomposition

Styrax sumatranus J.J.Sm endophytes fungi and its potential as phosphate solubilizing and cellulose decomposition

Karakterisasi Fungi Selulotik Dari Serasah Daun Mangrove Asal Hutan Mangrove Lamnga Kabupaten Aceh Besar

Morphological identification of phosphate solubilizing and cellulolytic fungi from mangrove soil under Rhizophora stylosa stands

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