Isolamento e caracterização de células-tronco mesenquimais de filtros reutilizáveis e descartáveis de medula óssea

Deus, Glaziane Cordeiro de; Normanton, Marilia; Hamerschlak, Nelson; Kondo, Andrea Tiemi; Ribeiro, Andreza Alice Feitosa; Goldberg, Anna Carla; Marti, Luciana Cavalheiro

doi:10.1590/s1679-45082012000300007

Cited by 8 publications

(4 citation statements)

References 21 publications

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“…hMSCs were obtained from health bone marrow donors, by washing the cells trapped in the dischargeable collection filter as previously described by Deus et al [22]. The lymphocytes were obtained from health donor’s peripheral blood following the signing of informed consent (Approved by Hospital Albert Einstein Ethical Committee -10/1412; CAAE: 06592712.4.0000.0071).…”

Section: Methodsmentioning

confidence: 99%

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Interleukin 7 Plays a Role in T Lymphocyte Apoptosis Inhibition Driven by Mesenchymal Stem Cell without Favoring Proliferation and Cytokines Secretion

et al. 2014

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Since 2004, when a case report describing the use of human mesenchymal stem cells (hMSCs) infusion as a therapy for GVHD after bone marrow transplantation, a new perspective in MSC function emerged. Since then hMSCs immunomodulatory potential became the target of several studies. Although great progress has been made in our understanding of hMSCs, their effect on T cell remains obscure. Our study has confirmed the already described effect of hMSCs on lymphocytes proliferation and survival. We also show that the impairment of lymphocyte proliferation and apoptosis is contact-independent and occurs in a prostaglandin-independent manner. A potential correlation between IL-7 and hMSCs effect is suggested, as we observed an increase in IL-7 receptors (CD127) on lymphocyte membrane in MSC presence. Additionally, blocking IL-7 in hMSCs-lymphocytes co-cultures increased lymphocytes apoptosis and we also have demonstrated that hMSCs are able to produce this interleukin. Moreover, we found that during Th1/Th17 differentiation in vitro, hMSCs presence leads to Th1/Th17 cells with reduced capacity of INF-y and IL-17 secretion respectively, regardless of having several pro-inflammatory cytokines in culture. We did not confirm an increment of Treg in these cultures, but a reduced percentage of INF-y/IL-17 secreting cells was observed, suggesting that the ratio between anti and pro-inflammatory cells changed. This changed ratio is very important to GvHD therapy and links hMSCs to an anti-inflammatory role. Taken together, our findings provide important preliminary results on the lymphocyte pathway modulated by MSCs and may contribute for developing novel treatments and therapeutic targets for GvHD and others autoimmune diseases.

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Section: Methodsmentioning

confidence: 99%

“…hMSCs were characterized by flow cytometry and checked for the ability to differentiate in osteocytes, chondrocytes or adipocytes as previously published by our group and briefly described below [22].…”

Section: Methodsmentioning

confidence: 99%

Interleukin 7 Plays a Role in T Lymphocyte Apoptosis Inhibition Driven by Mesenchymal Stem Cell without Favoring Proliferation and Cytokines Secretion

et al. 2014

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“…Deus et al (2012) destacam que estas células-tronco mesenquimais são obtidas principalmente da medula óssea, porém há relatos de caso destas células terem sido isoladas de cordão umbilical, fluido amniótico, tecido adiposo, músculo e fígado. Elas possuem a capacidade de se diferenciar em várias linhagens de células como células musculares, células epiteliais, células neurais, hepatócitos, tecido conjuntivo, ossos, entre outros (BYDLOWSKI et al, 2009;CABRAL et al, 2008;ARGOLO NETO;CARLO, 2010).…”

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A Utilização de Células-tronco em Doenças Cardíacas: Apresentação dos Estudos Publicados no Brasil

et al. 2015

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RESUMO:As doenças relacionadas ao músculo cardíaco e suas derivações representam grande parte da mortalidade no Brasil ao decorrer dos anos, por isso a utilização de células-tronco em doenças cardíacas vem sendo estudada através de ensaios clínicos realizados por pesquisadores na área da saúde. Objetivando descrever os resultados obtidos e publicados até o ano atual nas pesquisas de restauração funcional do tecido cardíaco, neste trabalho foi realizado um levantamento bibliográfico nas principais bases de dados: Scielo e Ebsco, publicadas no período de 2003 a 2014, com os seguintes descritores: Células-tronco, doenças cardíacas, terapia celular, transplante celular, infarto agudo do miocárdio, doença de Chagas, cardiomiopatia isquêmica e cardiomiopatia dilatada. Os resultados obtidos nos ensaios de utilização de células-tronco em doenças cardíacas têm se mostrado promissores, porém há muitos fatores ainda desconhecidos pela escassez de dados obtidos através dessas pesquisas a respeito de reação imunológica, dosagem correta, método ideal, tipo celular mais adequado entre outros parâmetros a serem testados a fim de aplicar ampla e efetivamente este novo tratamento, além da existência de divergências entre resultados obtidos por diferentes pesquisadores. PALAVRAS-CHAVE:Células-tronco; Doenças Cardíacas; Terapia Celular; Transplante Celular. STEM CELLS IN HEART DISEASES: REVIEW OF STUDIES PUBLISHED IN BRAZILABSTRACT: Diseases of the heart muscle and its derivatives account for most of the mortality rates in Brazil. The use of stem cells in heart diseases is currently studied by clinical tests undertaken by health professionals. Current analysis describes results published up to the present on the functional restoration of the heart tissue, by means of a bibliographical survey retrieved from SciELO and Ebsco data bases between 2003 and 2014. The descriptors stem cells, heart diseases, cell therapy, cell transplant, acute infarction, Chagas disease, ischemic cardiomyopathy and extended cardiomyopathy were researched.Results of assays on the use of stem cells in heart diseases are highly promising even though several unknown factors exist due to the scarcity of data on immunological reaction, correct doses, best method, the most adequate cell type and others. The above are parameters that should be tested to apply safely the new treatment, even though divergences exist in results obtained by different researchers.

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“…Na primeira fase deste estudo, nós demonstramos que células-tronco mesenquimais humanas (MSCs) podem ser obtidas para utilização em pesquisa cientifica a partir da lavagem de filtros de coletas de medula óssea, reutilizáveis ou descartáveis (121).…”

Section: Discussionunclassified

Estudo da interação das células-tronco mesenquimais e linfócitos no modelo da doença do enxerto contra hospedeiro

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Isolamento e caracterização de células-tronco mesenquimais de filtros reutilizáveis e descartáveis de medula óssea

Cited by 8 publications

References 21 publications

Interleukin 7 Plays a Role in T Lymphocyte Apoptosis Inhibition Driven by Mesenchymal Stem Cell without Favoring Proliferation and Cytokines Secretion

Interleukin 7 Plays a Role in T Lymphocyte Apoptosis Inhibition Driven by Mesenchymal Stem Cell without Favoring Proliferation and Cytokines Secretion

A Utilização de Células-tronco em Doenças Cardíacas: Apresentação dos Estudos Publicados no Brasil

Estudo da interação das células-tronco mesenquimais e linfócitos no modelo da doença do enxerto contra hospedeiro

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