Isoforms of acetyl-CoA carboxylase: structures, regulatory properties and metabolic functions

Brownsey, Roger W.; Zhande, Rachel; Boone, Adrienne N.

doi:10.1042/bst0251232

Cited by 61 publications

(56 citation statements)

References 14 publications

(23 reference statements)

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“…ACC is a biotinyl-enzyme and catalyses the rate limiting step in the biogenesis of long-chain fatty-acids (for reviews, see Brownsey et al, 1997;Kim, 1997). Two forms of ACC encoded by separate genes, have been identified: the 265-kDa ACCA and the 280-kDa ACCB (Thampy, 1989;Bianchi et al, 1990).…”

Section: Discussionmentioning

confidence: 99%

BRCA1 interacts with acetyl-CoA carboxylase through its tandem of BRCT domains

et al. 2002

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Germ-line alterations in BRCA1 are associated with an increased susceptibility to breast and ovarian cancer. BRCA1 is a 220-kDa protein that contains a tandem of two BRCA1 C-Terminal (BRCT) domains. Among missense and nonsense BRCA1 mutations responsible for family breast cancer, some are located into the BRCT tandem of BRCA1 coding sequence. In an attempt to understand how BRCT is critical for BRCA1 function, we search for partners of this BRCT tandem of BRCA1. Using a glutathione-S-transferase (GST) pull-down assay with murine cells, we isolated only one major BRCA1-interacting protein, further identified as Acetyl Coenzyme A (CoA) Carboxylase a (ACCA). We showed that this interaction is conserved through murine and human species. We also delineated the minimum interacting region as being the whole tandem of BRCT domains. We demonstrated that BRCA1 interacts in vitro and in vivo with ACCA. This interaction is completely abolished by five distinct germline BRCA1 deleterious mutations affecting the BRCT tandem of BRCA1. Interestingly, ACCA originally known as a rate-limiting enzyme for fatty acids biosynthesis, has been recently shown to be overexpressed in breast cancers and considered as a potential target for anti-neoplastic therapy. Furthermore, our observation is making a bridge between the genetic factors involved in susceptibility to breast and ovarian cancers, and environmental factors such as nutrition considered as key elements in the etiology of those cancers.

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Section: Discussionmentioning

confidence: 99%

BRCA1 interacts with acetyl-CoA carboxylase through its tandem of BRCT domains

et al. 2002

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“…It is a substrate for fatty acid synthases (4,16), for polyketide synthases in plants, fungi, and bacteria (19), and for fatty acid chain elongation systems (37). It also plays a role as a modulator of the activity of some proteins (8). Since malonyl-CoA is used in the production of many of the pharmaceutically important polyketides made by streptomycetes (19), there is considerable interest in understanding the pathway(s) that leads to its synthesis.…”

mentioning

confidence: 99%

Role of an Essential Acyl Coenzyme A Carboxylase in the Primary and Secondary Metabolism of Streptomyces coelicolor A3(2)

Rodrı́guez

Banchio

Diacovich

et al. 2001

Appl Environ Microbiol

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Two genes, accB and accE, that form part of the same operon, were cloned from Streptomyces coelicolor A3(2). AccB is homologous to the carboxyl transferase domain of several propionyl coezyme A (CoA) carboxylases and acyl-CoA carboxylases (ACCases) of actinomycete origin, while AccE shows no significant homology to any known protein. Expression of accB and accE in Escherichia coli and subsequent in vitro reconstitution of enzyme activity in the presence of the biotinylated protein AccA1 or AccA2 confirmed that AccB was the carboxyl transferase subunit of an ACCase. The additional presence of AccE considerably enhanced the activity of the enzyme complex, suggesting that this small polypeptide is a functional component of the ACCase. The impossibility of obtaining an accB null mutant and the thiostrepton growth dependency of a tipAp accB conditional mutant confirmed that AccB is essential for S. coelicolor viability. Normal growth phenotype in the absence of the inducer was restored in the conditional mutant by the addition of exogenous long-chain fatty acids in the medium, indicating that the inducer-dependent phenotype was specifically related to a conditional block in fatty acid biosynthesis. Thus, AccB, together with AccA2, which is also an essential protein (E. Rodriguez and H. Gramajo, Microbiology 143:3109-3119, 1999), are the most likely components of an ACCase whose main physiological role is the synthesis of malonyl-CoA, the first committed step of fatty acid synthesis. Although normal growth of the conditional mutant was restored by fatty acids, the cultures did not produce actinorhodin or undecylprodigiosin, suggesting a direct participation of this enzyme complex in the supply of malonyl-CoA for the synthesis of these secondary metabolites.

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“…ACCase is essential for the malonyl-CoA supply of fatty acid biosynthesis (Brownsey et al, 1997), with high ACCase mRNAs tissue accumulations indicating highly active of fatty acids biosynthesis (Ke et al, 2000a). The Q-PCR results of the current study showed that the changes in expression of ACCase genes (ACC, α-CT, and β-CT) were negatively correlated with changes in oil content in response to GA 3 .…”

Section: Oil Biosynthesis Related Genesmentioning

confidence: 53%

Effect of exogenous gibberellin on reserve accumulation during the seed filling stage of oilseed rape

Huang¹,

He²,

Liao³

et al. 2014

Genet. Mol. Res.

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ABSTRACT. Exogenous gibberellins (GAs) are widely applied to increase crop yields, with knowledge about the physiological functioning and biochemistry mechanisms of these phytohormones improving; however, information remains limited about the effect of GAs on seed filling. In this study, the siliques (containing the seeds) of oilseed rape (Brassica napus L.) were treated with GA 3 at 3 stages of seed filling. We confirmed that GA 3 regulates the deposition of storage reserves in developing seeds. The percentage of crude fat in the seeds increased during the early stage, but remained stable during the middle and late stages. In comparison, the percentage of total protein decreased during the early and middle stages, but significantly increased during the late stage. In addition, Q-PCR was employed to analyze the expression level of related genes in 2828 X.Q. Huang et al.©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 13 (2): 2827-2839 (2014) response to GA 3 . It was found that the expression of WRI and ABI3 transcription factors corresponded to crude fat content and total protein content, respectively. The expression of storage reserve related genes DGAT, MCAT, SUC2, and GPT was consistent with crude fat content, whereas the expression of Napin corresponded to total protein content. The results of this study indicate that exogenous GA 3 has a different effect on storage reserve deposition in seed during different stages of seed filling, and the effect might be achieved via changing the expression of related genes.

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Isoforms of acetyl-CoA carboxylase: structures, regulatory properties and metabolic functions

Cited by 61 publications

References 14 publications

BRCA1 interacts with acetyl-CoA carboxylase through its tandem of BRCT domains

BRCA1 interacts with acetyl-CoA carboxylase through its tandem of BRCT domains

Role of an Essential Acyl Coenzyme A Carboxylase in the Primary and Secondary Metabolism of Streptomyces coelicolor A3(2)

Effect of exogenous gibberellin on reserve accumulation during the seed filling stage of oilseed rape

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