Isoform switching of Cdc6 contributes to developmental cell cycle remodeling

Tikhmyanova, Nadia; Coleman, Thomas R.

doi:10.1016/s0012-1606(03)00253-7

Cited by 13 publications

(17 citation statements)

References 40 publications

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“…We note that two XCdc6 isoforms (XCdc6A and XCdc6B) have been identified, and XCdc6A is down-regulated after the MBT44. The two isoforms, which are named as XCdc6 protein and Cdc6B protein in the protein database, can be placed into two different protein groups in the protein identification lists (supplementary material II).…”

Section: Resultsmentioning

confidence: 99%

Quantitative proteomics of Xenopus laevis embryos: expression kinetics of nearly 4000 proteins during early development

Sun

Bertke

Champion

et al. 2014

Sci Rep

103

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While there is a rich literature on transcription dynamics during the development of many organisms, protein data is limited. We used iTRAQ isotopic labeling and mass spectrometry to generate the largest developmental proteomic dataset for any animal. Expression dynamics of nearly 4,000 proteins of Xenopus laevis was generated from fertilized egg to neurula embryo. Expression clusters into groups. The cluster profiles accurately reflect the major events that mark changes in gene expression patterns during early Xenopus development. We observed decline in the expression of ten DNA replication factors after the midblastula transition (MBT), including a marked decline of the licensing factor XCdc6. Ectopic expression of XCdc6 leads to apoptosis; temporal changes in this protein are critical for proper development. Measurement of expression in single embryos provided no evidence for significant protein heterogeneity between embryos at the same stage of development.

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Section: Resultsmentioning

confidence: 99%

Quantitative proteomics of Xenopus laevis embryos: expression kinetics of nearly 4000 proteins during early development

Sun

Bertke

Champion

et al. 2014

Sci Rep

103

View full text Add to dashboard Cite

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“…One possibility is that during evolution, these duplicated genes acquired different functions and/or involvement in different stages during development. In X. laevis, the two isoforms of CDC6 are functional and are expressed in different developmental stages [34]. Similarly, AtCDC6a and AtCDC6b expression pattern is different in plant organs, suggesting different roles in development.…”

Section: Discussionmentioning

confidence: 99%

Genome based identification and analysis of the pre‐replicative complex of Arabidopsis thaliana

et al. 2004

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Eukaryotic DNA replication requires an ordered and regulated machinery to control G1/S transition. The formation of the pre-replicative complex (pre-RC) is a key step involved in licensing DNA for replication. Here, we identify all putative components of the full pre-RC in the genome of the model plant Arabidopsis thaliana. Different from the other eukaryotes, Arabidopsis houses in its genome two putative homologs of ORC1, CDC6 and CDT1. Two mRNA variants of AtORC4 subunit, with different temporal expression patterns, were also identified. Two-hybrid binary interaction assays suggest a primary architectural organization of the Arabidopsis ORC, in which AtORC3 plays a central role in maintaining the complex associations. Expression profiles differ among pre-RC components suggesting the existence of various forms of the complex, possibly playing different roles during development. In addition, the expression of the putative pre-RC genes in non-proliferating plant tissues suggests that they might have roles in processes other than DNA replication licensing.

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“…Similarly, AtCDC6A (At2g29680) and AtCDC6B (At1g07270) have distinct expression profiles (Masuda et al, 2004). The only other eukaryote known to have multiple CDC6 genes is Xenopus laevis, where XlCDC6A and XlCDC6B have distinct N-terminal regulatory motifs and different expression patterns in the developing frog embryo (Tikhmyanova and Coleman, 2003). XlCDC6A acts prior to the midblastula transition, after which XlCDC6B becomes the dominant protein (Tikhmyanova and Coleman, 2003).…”

Section: Okazaki Fragment Maturationmentioning

confidence: 99%

“…The only other eukaryote known to have multiple CDC6 genes is Xenopus laevis, where XlCDC6A and XlCDC6B have distinct N-terminal regulatory motifs and different expression patterns in the developing frog embryo (Tikhmyanova and Coleman, 2003). XlCDC6A acts prior to the midblastula transition, after which XlCDC6B becomes the dominant protein (Tikhmyanova and Coleman, 2003). The midblastula transition coincides with extensive chromatin remodeling, activation of zygotic transcription, and a clear shift in the regulation of origin usage.…”

Section: Okazaki Fragment Maturationmentioning

confidence: 99%

Genome-Wide Analysis of the Core DNA Replication Machinery in the Higher Plants Arabidopsis and Rice

et al. 2007

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Core DNA replication proteins mediate the initiation, elongation, and Okazaki fragment maturation functions of DNA replication. Although this process is generally conserved in eukaryotes, important differences in the molecular architecture of the DNA replication machine and the function of individual subunits have been reported in various model systems. We have combined genome-wide bioinformatic analyses of Arabidopsis (Arabidopsis thaliana) and rice (Oryza sativa) with published experimental data to provide a comprehensive view of the core DNA replication machinery in plants. Many components identified in this analysis have not been studied previously in plant systems, including the GINS (go ichi ni san) complex (PSF1, PSF2, PSF3, and SLD5), MCM8, MCM9, MCM10, NOC3, POLA2, POLA3, POLA4, POLD3, POLD4, and RNASEH2. Our results indicate that the core DNA replication machinery from plants is more similar to vertebrates than single-celled yeasts (Saccharomyces cerevisiae), suggesting that animal models may be more relevant to plant systems. However, we also uncovered some important differences between plants and vertebrate machinery. For example, we did not identify geminin or RNASEH1 genes in plants. Our analyses also indicate that plants may be unique among eukaryotes in that they have multiple copies of numerous core DNA replication genes. This finding raises the question of whether specialized functions have evolved in some cases. This analysis establishes that the core DNA replication machinery is highly conserved across plant species and displays many features in common with other eukaryotes and some characteristics that are unique to plants.DNA replication depends on the coordinated action of numerous multiprotein complexes. At the simplest level, it requires an initiator to establish the site of replication initiation, a helicase to unwind DNA, a polymerase to synthesize new DNA, and machinery to process the Okazaki fragments generated during discontinuous synthesis. Much is known about the DNA replication machinery in yeast (Saccharomyces cerevisiae) and animal model systems, but relatively little is known about the apparatus in plants. To gain insight into plant DNA replication components, we have combined published experimental information with our own bioinformatic analysis of genomic sequence data to examine the core DNA replication machinery in the model plants Arabidopsis (Arabidopsis thaliana) and rice (Oryza sativa).Figure 1 depicts a model eukaryotic DNA replication fork and illustrates the protein complexes known or suspected to be part of the core DNA replication machine. These complexes mediate the initiation, elongation, and maturation stages of DNA replication and, as such, constitute the core eukaryotic DNA replication machinery. The events leading to the formation of an active DNA replication fork occur in a stepwise fashion, but our understanding of the timing and specific details of how these events unfold in diverse eukaryotes is limited, and there are a growing number of examples of vari...

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Isoform switching of Cdc6 contributes to developmental cell cycle remodeling

Cited by 13 publications

References 40 publications

Quantitative proteomics of Xenopus laevis embryos: expression kinetics of nearly 4000 proteins during early development

Quantitative proteomics of Xenopus laevis embryos: expression kinetics of nearly 4000 proteins during early development

Genome based identification and analysis of the pre‐replicative complex of Arabidopsis thaliana

Genome-Wide Analysis of the Core DNA Replication Machinery in the Higher Plants Arabidopsis and Rice

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