Isoform-specific regulation of the lactate transporters MCT1 and MCT4 by contractile activity

Bonen, Arend; Tonouchi, Mio; Miskovic, Dragana; Heddle, Catherine; Heikkila, John J.; Halestrap, Andrew P.

doi:10.1152/ajpendo.2000.279.5.e1131

Cited by 98 publications

(151 citation statements)

References 49 publications

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“…5 ' 6 In humans, expression of MCTl may correlates with lactate removal 7 ' 8 and with the amount of lactate oxidised into muscle cells. 9 In humans, defects in MCTl transporter have been associated with several pathologies. Symptoms and signs of muscle injury on exercise and heat exposure plus subnormal erythrocyte lactate transport have been related with the presence of two mutations in the gene that codifies MCTl.…”

Section: Introductionmentioning

confidence: 99%

MCT1 genetic polymorphism influence in high intensity circuit training: A pilot study

Cupeiro

Benito

Maffulli

et al. 2010

Journal of Science and Medicine in Sport

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Monocarboxylate Transporter 1 (MCTl) mediates the transport of the main fraction of lactate across the sarcolemma. A common polymorphic MCTl variant has been identified, but its role in high intensity exercise performance has not been defined. We investigated the influence of MCTl A1470T polymorphism (rsl049434) on lactate accumulation after high intensity circuit training. Ten men aged 20-26 performed three controlled circuit training (CWT) sessions at 60%, 70%, and 80% of the 15 repetition maximum (15 RM), in non-consecutive days. CWT included three sets of a circuit of eight exercises, obtaining lactate measurements immediately after each set had been completed. Two independent variables were analysed: MTC1 genotypes according to the presence or absence of the A1470T polymorphism, and the intensity of circuit training. Genotype distributions were in Hardy-Weinberg equilibrium, being 30% wild-type, 50% heterozygotes, and 20% mutated homozygotes. Mean lactate concentration at 80% of 15RM were significantly higher than the mean lactate values at the other intensities (p < 0.01). Significant differences between genetic groups were found in the lactate accumulation slope at 80% of 15 RM (p = 0.02) and in the maximal lactate concentration reached by all subjects in the study (L max ) (p = 0.03). The carriers of the A1470T polymorphism in the MTC1 gene seem to exhibit a worse lactate transport capability into the less active muscle cells for oxidation.

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Section: Introductionmentioning

confidence: 99%

MCT1 genetic polymorphism influence in high intensity circuit training: A pilot study

Cupeiro

Benito

Maffulli

et al. 2010

Journal of Science and Medicine in Sport

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“…MCT facilitates lactate transport across the plasma membrane. Endurance exercise training increases MCT1 expression, which facilitates lactate uptake into muscle cells (16). Endurance training also increases muscle glycogen concentration, but it remains unknown whether increases in MCT1 protein expression are associated with glycogen concentration after training.…”

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confidence: 99%

Chronic Post-Exercise Lactate Administration with Endurance Training Increases Glycogen Concentration and Monocarboxylate Transporter 1 Protein in Mouse White Muscle

Hoshino

Hanawa

Takahashi

et al. 2014

J Nutr Sci Vitaminol

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“…Numerous studies have shown that lactate transport and MCT expression in muscle could be regulated by physical activity and electrical stimulation in nonpathological states (3,9). Recently it has been shown that caloric restriction could also affect lactate transport activity in rats (20).…”

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confidence: 99%

Effect of weight loss on lactate transporter expression in skeletal muscle of obese subjects

Metz¹,

Mercier²,

Tremblay³

et al. 2008

Journal of Applied Physiology

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DR. Effect of weight loss on lactate transporter expression in skeletal muscle of obese subjects. J Appl Physiol 104: 633-638, 2008. First published December 13, 2007 doi:10.1152/japplphysiol.00681.2007.-The effects of weight loss on skeletal muscle lactate transporter [monocarboxylate transporter (MCT)] expression in obese subjects were investigated to better understand how lactate transporter metabolism is regulated in insulin-resistant states. Ten obese subjects underwent non-macronutrient-specific energy restriction for 15 wk. Anthropometric measurements and a needle biopsy of the vastus lateralis muscle before and after the weight loss program were performed. Enzymatic activity, fiber type distribution, and skeletal muscle MCT protein expression were measured. Muscle from nonobese control subjects was used for comparison of MCT levels. The program induced a weight loss of 9.2 Ϯ 1.6 kg. Compared with controls, muscle from obese subjects showed a strong tendency (P ϭ 0.06) for elevated MCT4 expression (ϩ69%) before the weight loss program. MCT4 expression decreased (Ϫ7%) following weight loss to reach levels that were not statistically different from control levels. There were no differences in MCT1 expression between controls and obese subjects before and after weight loss. A highly predictive regression model (R 2 ϭ 0.93), including waist circumference, citrate synthase activity, and percentage of type 1 fibers, was found to explain the highly variable MCT1 response to weight loss in the obese group. Therefore, in obesity, MCT1 expression appears linked both to changes in oxidative parameters and to changes in visceral adipose tissue content. The strong tendency for elevated expression of muscle MCT4 could reflect the need to release greater amounts of muscle lactate in the obese state, a situation that would be normalized with weight loss as indicated by decreased MCT4 levels. monocarboxylate transporters; lactate transport; diet THE PREVALENCE OF OBESITY and associated comorbidities is increasing (30,40), underscoring the importance of developing effective strategies for reducing obesity and the risk of metabolic disease linked to insulin resistance. Diet-induced weight loss can promote significant improvement in clinical and metabolic parameters (14,19) and plays a role in the prevention of Type 2 diabetes. This therapeutic approach can also act at the cellular level, improving glucose metabolism. For example, Dean et al. (6) showed that dietary restriction could increase insulin-stimulated glucose transport by enhancing the proportion of GLUT-4 at the muscle cell surface.It has been shown that in insulin-resistant states there is an increase in plasma lactate levels at rest (22). Lactate and glucose are closely related since the former is an intermediate metabolite of the latter, and hyperlactatemia has been recognized as an independent risk factor for the development and aggravation of insulin resistance (31). Moreover, lactate is now recognized as an important substrate for ATP production in several...

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Isoform-specific regulation of the lactate transporters MCT1 and MCT4 by contractile activity

Cited by 98 publications

References 49 publications

MCT1 genetic polymorphism influence in high intensity circuit training: A pilot study

MCT1 genetic polymorphism influence in high intensity circuit training: A pilot study

Chronic Post-Exercise Lactate Administration with Endurance Training Increases Glycogen Concentration and Monocarboxylate Transporter 1 Protein in Mouse White Muscle

Effect of weight loss on lactate transporter expression in skeletal muscle of obese subjects

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