Isoform‐specific expression and characterization of 14‐3‐3 proteins in human glioma tissues discovered by stable isotope labeling with amino acids in cell culture‐based proteomic analysis

Liang, Shufang; Shen, Guobo; Liu, Qingping; Xu, Yingxi; Zhou, Liangxue; Xiao, Shiyin; Xu, Zhaochun; Gong, Fengming; You, Chao; Wei, Yuquan

doi:10.1002/prca.200800198

Cited by 37 publications

(36 citation statements)

References 65 publications

(85 reference statements)

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“…The effect of 14-3-3 proteins on cancer development and their interaction with various oncogenes and tumor suppressor genes is complex (36). A previous study investigated the increased immune reactivity of various 14-3-3 isoforms in human astrocytoma samples, and the immunoreactivity of cells was significantly enhanced with increased disease progression (37).…”

Section: Discussionmentioning

confidence: 99%

Molecular mechanisms of the effect of TGF-β1 on U87 human glioblastoma cells

Bryukhovetskiy

Шевченко

2016

Oncology Letters

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Abstract. Glioblastoma multiforme (GBM) is the most widespread and aggressive type of primary brain tumor. The prognosis following diagnosis with GBM is poor, with a median survival time of 14 months. Tumor cell invasion, metastasis and proliferation are the major causes of mortality in patients with GBM. In order to develop effective GBM treatment methods it is necessary to identify novel targets involved in these processes. Recently, there has been increasing interest in investigating the signaling pathways involved in GBM development, and the transforming growth factor-β (TGF-β) signaling pathway is understood to be significant for regulating the behavior of GBM, as well as stimulating its invasion and metastatic development. Particular interest has been given to investigating the modulation of TGF-β-induced epithelial-to-mesenchymal transition (EMT); during this process, epithelial cells transdifferentiate into mobile cells with a mesenchymal phenotype. The induction of EMT increases the invasiveness of various types of carcinoma; however, the role of TGF-β in this process remains to be elucidated, particularly in the case of GBM. The current study presents a comparative proteome mapping of the U87 human glioblastoma cell line, with and without TGF-β1 treatment. Proteome analysis identified numerous proteins involved in the molecular mechanisms of GBM oncogenesis and TGF-β1 signaling in glioblastoma. The results of the present study facilitated the identification of novel potential markers of metastasis and candidates for targeted glioblastoma therapy, which may potentially be validated and used in clinical medicine to develop improved approaches for GBM diagnosis and treatment.

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Section: Discussionmentioning

confidence: 99%

Molecular mechanisms of the effect of TGF-β1 on U87 human glioblastoma cells

Bryukhovetskiy

Шевченко

2016

Oncology Letters

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“…10 In addition, 14-3-3␤ and 14-3-3 were found specifically expressed in human astrocytoma. 11,12 The expression frequency and levels of 14-3-3␤ and 14-3-3 were associated with human astrocytoma. 12 Moreover, increased expression of 14-3-3 in breast cancer was shown in a proteomic study.…”

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confidence: 99%

Increased Expression of 14-3-3β Promotes Tumor Progression and Predicts Extrahepatic Metastasis and Worse Survival in Hepatocellular Carcinoma

Liu

Jan

et al. 2011

The American Journal of Pathology

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14-3-3␤ is implicated in cell survival, proliferation, migration, and tumor growth; however, its clinical relevance in tumor progression and metastasis have never been elucidated. To evaluate the clinical significance of 14-3-3␤, we analyzed the association of 14-3-3␤ expression and clinicopathologic characteristics in primary and subsequent metastatic tumors of hepatocellular carcinoma patients. 14-3-3␤ was expressed abundantly in 40 of 55 (70.7%) primary tumors. Increased 14-3-3␤ expression in primary tumors predicted a higher 5-year cumulative incidence of subsequent extrahepatic metastasis, and multivariate analysis revealed 14-3-3␤ overexpression was an independent risk factor for extrahepatic metastasis. Patients with increased 14-3-3␤ expression in primary tumors had worse 5-year overall survival rates, and 14-3-3␤ overexpression was an independent prognostic factor on Cox regression analysis. Furthermore, stably overexpressed 14-3-3␤ enhanced hepatocellular carcinoma cell migration and proliferation and increased anchorage-independent cell growth. In addition, in vivo study in a nude-mice model showed tumor formation significantly increased with 14-3-3␤ overexpression. In conclusion, this is the first report to show that increased 14-3-3␤ expression is associated with subsequent extrahepatic metastasis and worse survival rates, as well as cancer progression of hepatocellular carcinoma. Thus, 14-3-3␤ may be a novel prognostic biomarker and therapeutic target in hepatocellular carcinoma.

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“…Роль белков 14-3-3 в злокачественных опухолях и их взаимодействие с различным опухолеродными генами и генами-супрессорами опухоли сложна и ма-ло изучена [32]. Предыдущие исследования продемон-стрировали, что иммунореактивность изоформ 14-3-3b, 3, d, q, h и z наблюдалась в большинстве образцов с астро-цитом, и их иммунореактивные показатели заметно увеличивались с ростом стадии заболевания [33].…”

Section: результатыunclassified