Isoflurane Preconditioning Promotes the Survival and Migration of Bone Marrow Stromal Cells

Sun, Yinghao; Li, Qifang; Jia, Yan; Hu, Rong; Jiang, Hong

doi:10.1159/000430300

Cited by 21 publications

(28 citation statements)

References 49 publications

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“… [ 91 ] ISO Bone marrow Stroke model In vitro / in vivo (rats) Upregulated CXCR4 and HIF-1α expression; improved engraftment into the ischemic brain and improved functional recovery. [ 92 ] CCPA Dental pulp Osteogenesis In vitro Improved proliferation and osteogenic differentiation; upregulated RUNX-2 and alkaline phosphatase expression; improved mineralization in extracellular matrix. [ 96 ] ATRA Bone marrow (rat) Excisional wounds model In vitro / in vivo (rats) Upregulated COX-2, HIF-1, CXCR4, CCR2, VEGF, Ang-2 and Ang-4 gene expression; improved wound healing.…”

Section: Msc Priming With Pharmacological or Chemical Agentsmentioning

confidence: 99%

“…In vitro preconditioning of BM-MSC with ISO (low doses and short treatment duration, 2% ISO for 4 h) increases cell viability and migration potential. There was hypoxia-induced upregulation of CXCR4 and HIF-1α, whereas no effect was observed in the expression of HIF-1β [ 92 ]. However, longer exposures to ISO at higher doses decreased MSC viability and migration potential.…”

Section: Msc Priming With Pharmacological or Chemical Agentsmentioning

confidence: 99%

“…However, longer exposures to ISO at higher doses decreased MSC viability and migration potential. In a rat model of stroke, preconditioned ISO-MSC improved the function and engraftment into the ischemic brain [ 92 ].…”

Section: Msc Priming With Pharmacological or Chemical Agentsmentioning

confidence: 99%

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Priming approaches to improve the efficacy of mesenchymal stromal cell-based therapies

et al. 2019

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Multipotent mesenchymal stromal cells (MSC) have been widely explored for cell-based therapy of immune-mediated, inflammatory, and degenerative diseases, due to their immunosuppressive, immunomodulatory, and regenerative potentials. Preclinical studies and clinical trials have demonstrated promising therapeutic results although these have been somewhat limited. Aspects such as low in vivo MSC survival in inhospitable disease microenvironments, requirements for ex vivo cell overexpansion prior to infusions, intrinsic differences between MSC and different sources and donors, variability of culturing protocols, and potency assays to evaluate MSC products have been described as limitations in the field. In recent years, priming approaches to empower MSC have been investigated, thereby generating cellular products with improved potential for different clinical applications. Herein, we review the current priming approaches that aim to increase MSC therapeutic efficacy. Priming with cytokines and growth factors, hypoxia, pharmacological drugs, biomaterials, and different culture conditions, as well as other diverse molecules, are revised from current and future perspectives.

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Section: Msc Priming With Pharmacological or Chemical Agentsmentioning

confidence: 99%

Section: Msc Priming With Pharmacological or Chemical Agentsmentioning

confidence: 99%

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Priming approaches to improve the efficacy of mesenchymal stromal cell-based therapies

et al. 2019

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“…Effects of BMSCs on improving neurological functions have been well studied in recent years and studies are ongoing into methods of increasing their viability after transplant [33,34]. BMSCs promote remyelination and accelerate conductive velocity of electrophysiology of demyelinated nerves [35].…”

Section: Discussionmentioning

confidence: 99%

Bone Marrow Stromal Cells Promote Neuronal Restoration in Rats with Traumatic Brain Injury: Involvement of GDNF Regulating BAD and BAX Signaling

Shen¹,

Yin²,

Xia³

et al. 2016

Cell Physiol Biochem

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Background/Aims: To investigate the effects of bone marrow stromal cells (BMSCs) and underlying mechanisms in traumatic brain injury (TBI). Methods: Cultured BMSCs from green fluorescent protein-transgenic mice were isolated and confirmed. Cultured BMSCs were immediately transplanted into the regions surrounding the injured-brain site to test their function in rat models of TBI. Neurological function was evaluated by a modified neurological severity score on the day before, and on days 7 and 14 after transplantation. After 2 weeks of BMSC transplantation, the brain tissue was harvested and analyzed by microarray assay. And the coronal brain sections were determined by immunohistochemistry with mouse anti-growth-associated protein-43 kDa (anti-GAP-43) and anti-synaptophysin to test the effects of transplanted cells on the axonal regeneration in the host brain. Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay and Western blot were used to detect the apoptosis and expression of BAX and BAD. Results: Microarray analysis showed that BMSCs expressed growth factors such as glial cell-line derived neurotrophic factor (GDNF). The cells migrated around the injury sites in rats with TBI. BMSC grafts resulted in an increased number of GAP-43-immunopositive fibers and synaptophysin-positive varicosity, with suppressed apoptosis. Furthermore, BMSC transplantation significantly downregulated the expression of BAX and BAD signaling. Moreover, cultured BMSC transplantation significantly improved rat neurological function and survival. Conclusion: Transplanted BMSCs could survive and improve neuronal behavior in rats with TBI. Mechanisms of neuroprotection and regeneration were involved, which could be associated with the GDNF regulating the apoptosis signals through BAX and BAD.

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“…Bone marrow mesenchymal stem cells (BMSCs) could differentiate into various cell types because they are multi-potent and have a self-renewing capacity [13]. A previous study demonstrated that BMSC transplantation is effective in acute brain injury by increasing neurotrophic factors and promoting functional recovery [14].…”

Section: Introductionmentioning

confidence: 99%

Bone Marrow Mesenchymal Stem Cell Transplantation Increases GAP-43 Expression via ERK1/2 and PI3K/Akt Pathways in Intracerebral Hemorrhage

Chen

Cui

et al. 2017

Cell Physiol Biochem

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Background/Aims: Intracerebral hemorrhage (ICH) occurs in hypertensive patients and results in high rates of mortality and disability. This study determined whether bone marrow mesenchymal stem cell (BMSC) transplantation affects axonal regeneration and examined the underlying mechanisms after the administration of PD98059 (p-ERK1/2 inhibitor) or/ and LY294002 (PI3K inhibitor). The hypothesis that was intended to be tested was that BMSC transplantation regulates the expression of growth-associated protein-43 (GAP-43) via the ERK1/2 and PI3K/Akt signaling pathways. Methods: Seventy-five male rats (250-280 g) were subjected to intracerebral blood injection and then randomly received a vehicle, BMSCs, PD98059 or LY294002 treatment. Neurological deficits were evaluated prior to injury and at 1, 3 and 7 days post-injury. The expression of GAP-43, Akt, p-Akt, ERK1/2, and p-ERK1/2 proteins was measured by western blot analysis. Results: BMSC transplantation attenuated neurological deficits 3-7 days post-ICH. The expression of GAP-43 was increased 3 days following BMSC transplantation. However, this increase was inhibited by either PD98059 or LY294002 treatment. Treatment with both PD98059 and LY294002 was more effective than was treatment with an individual compound. Conclusion: BMSC transplantation could attenuate neurological deficits and activate axonal regeneration in this rat ICH model. The protective effects might be associated with increased GAP-43 expression by activating both the ERK1/2 and PI3K/Akt signaling pathways.J. Cui and C. Cui contributed equally to this work as co-first authors.

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Isoflurane Preconditioning Promotes the Survival and Migration of Bone Marrow Stromal Cells

Cited by 21 publications

References 49 publications

Priming approaches to improve the efficacy of mesenchymal stromal cell-based therapies

Priming approaches to improve the efficacy of mesenchymal stromal cell-based therapies

Bone Marrow Stromal Cells Promote Neuronal Restoration in Rats with Traumatic Brain Injury: Involvement of GDNF Regulating BAD and BAX Signaling

Bone Marrow Mesenchymal Stem Cell Transplantation Increases GAP-43 Expression via ERK1/2 and PI3K/Akt Pathways in Intracerebral Hemorrhage

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