Isoflurane has no effect on cognitive or behavioral performance in a mouse model of early-stage Alzheimer’s disease

Borgstedt, Laura; Bratke, Sebastian; Blobner, Manfred; Pötzl, Christoph; Ulm, Bernhard; Jungwirth, Bettina; Schmid, Sebastian

doi:10.3389/fnins.2022.1033729

Cited by 5 publications

(9 citation statements)

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“…After general anesthesia, mice underwent neurocognitive testing for 8 consecutive days. Results from the post-anesthesia part of the study have already been published ( 18 ). Data on anesthesia in Tg2576 mice over a period of 120 min are not very frequently encountered in the literature, as general anesthesia in animal experiments is often maintained for a shorter time period and published data on vital parameters is limited ( 13 , 15 , 17 , 21 ).…”

Section: Discussionmentioning

confidence: 99%

“…At least 14 days prior to anesthesia, cognitive and behavioral testing, mice were transferred to a test facility for acclimatization. Mice were housed under standard laboratory conditions (specific pathogen free environment, 12 h light/12 h dark cycle, 22°C, 60% humidity and free access to water and standard mouse chow) ( 11 , 18 ).…”

Section: Methodsmentioning

confidence: 99%

“…Mice were randomly assigned to the experimental groups regarding isoflurane anesthesia or sham procedure using a computer-generated randomization list ( 18 ).…”

Section: Methodsmentioning

confidence: 99%

“…In a previously published study, our group investigated the influence of isoflurane anesthesia on neurocognition, behavior and amyloidopathy in 10 months old Tg2576 mice with respect to sex. Typical symptoms of early stage AD with corresponding histopathological alterations were found, whereas relevant sex-specific differences or an influence of isoflurane on AD symptomology and pathology could not be detected ( 18 ). We retrospectively assessed vital parameters during general anesthesia in order to address whether isoflurane affects heart rate, respiratory rate and body temperature dependent on transgenic status or sex.…”

Section: Introductionmentioning

confidence: 99%

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Genotype- and sex-specific changes in vital parameters during isoflurane anesthesia in a mouse model of Alzheimer’s disease

Bratke,

Schmid,

Ulm

et al. 2024

Front. Med.

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BackgroundThe prevalence of neurodegenerative diseases is increasing as is life expectancy with Alzheimer’s disease accounting for two-thirds of dementia cases globally. Whether general anesthesia and surgery worsen cognitive decline is still a matter of debate and most likely depending on the interplay of various influencing factors. In order to account for this complexity, Alzheimer’s disease animal models have been developed. The Tg2576 model of Alzheimer’s disease is a well-established mouse model exhibiting amyloidopathy and age-dependent sex-specific differences in Alzheimer’s disease symptomology. Yet, data on anesthesia in this mouse model is scarce and a systematic comparison of vital parameters during anesthesia with wild-type animals is missing. In order to investigate the safety of general anesthesia and changes in vital parameters during general anesthesia in Tg2576 mice, we did a secondary analysis of vital parameters collected during general anesthesia in aged Tg2576 mice.MethodsAfter governmental approval (General Administration of the Free State of Bavaria, file number: 55.2-1-54-2532-149-11) 60 mice at 10-12 months of age were exposed to isoflurane (1.6 Vol%) for 120 min, data of 58 mice was analyzed. During general anesthesia, heart rate, respiratory rate, temperature, isoflurane concentration and fraction of inspired oxygen were monitored and collected. Data were analyzed using univariate and multivariate linear mixed regression models.ResultsDuring general anesthesia, heart rate decreased in a sex-specific manner. Respiratory rate decreased and body temperature increased dependent on genotype. However, the changes were limited and all vital parameters stayed within physiological limits.ConclusionIsoflurane anesthesia in the Tg2576 mouse model is safe and does not seem to influence experimental results by interacting with vital parameters. The present study provides information on appropriate anesthesia in order to advance research on anesthesia and AD and could contribute to improving laboratory animal welfare.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…Mice were randomly assigned to the experimental groups regarding isoflurane anesthesia or sham procedure using a computer-generated randomization list ( 18 ).…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Genotype- and sex-specific changes in vital parameters during isoflurane anesthesia in a mouse model of Alzheimer’s disease

Bratke,

Schmid,

Ulm

et al. 2024

Front. Med.

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“…In this study, we used a concentration of 4.0% isoflurane which may affect cellular damage and thus affect cognitive function. 5,24) Finally, we did not fully investigate neuroinflammation. Neuroinflammation by itself or through interactions with Aβ protein and tau proteins can impair cognitive function.…”

Section: Discussionmentioning

confidence: 99%

Effect of Therapeutic Hypothermia on Cognitive Impairment in a Mouse Model of Ischemia-Reperfusion Injury

Lee¹,

Cho²,

Youn³

et al. 2023

J Korean Neurosurg Soc

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Objective : Therapeutic hypothermia (TH) is increasingly being used to reduce secondary brain damage and intracranial pressure occurring in the acute phase after mechanical thrombectomy. However, the effect of TH on cognitive impairment has yet to be elucidated. In this work, we investigated whether TH can improve cognitive impairment in a mouse model of cerebral ischemia/reperfusion injury.Methods : Nine-week-old C57BL/6N mice (male) were randomly assigned to three groups: sham control, transient middle cerebral artery occlusion (tMCAO), and tMCAO with TH. One month after model induction, the expression levels of amyloid-β (Aβ 1-42), tau, and cyclophilin A were obtained using western blot analysis, while cognitive functioning was tested using open field and Y-maze, and the results were compared based on treatment group.Results : Compared to sham mice, mice with tMCAO exhibited significantly increased expression of Aβ (1-42) in the hippocampus, temporal cortex, and basal forebrain, along with significantly increased expression of tau and cyclophilin A in the basal forebrain. Further, mice with tMCAO exhibited decreased function of learning and memory. TH significantly decreased the Aβ (1-42) in the basal forebrain (0.84 ± 0.24 vs. 1.47 ± 0.29 in tMCAO), Aβ (1-42) in the hippocampus (0.73 ± 0.15 vs. 1.79 ± 0.58 in tMCAO), and tau in the basal forebrain (1.14 ± 0.21 vs. 1.58 ± 0.98 in tMCAO), but not that of cyclophilin A. Cognitive functions were significantly improved in mice with tMCAO and TH compared to those with tMCAO. Conclusion :TH might be beneficial in ameliorating cognitive impairment after ischemia/reperfusion injury by reducing the expressions of Aβ (1-42) and tau.

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Neuroprotective Effect of Therapeutic Hypothermia in Cognitive Impairment of tMCAo Mouse Model

Lim,

Kim,

Park

et al. 2023

Preprint

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Therapeutic hypothermia (TH) has emerged as a promising approach with neuroprotective effects that can mitigate secondary brain damage and intracranial pressure in the acute phase after successful mechanical thrombectomy. However, the effect of TH on cognitive impairment has yet fully elucidated. In the present study, we demonstrated whether TH can improve cognitive impairment in a transient middle cerebral artery occlusion (tMCAO) mouse model Nine-week-old C57BL/6N mice (male) were randomized to three groups: sham, tMCAO, and tMCAO with TH. One month after model induction, cognitive function test and regional cerebral glucose metabolism were evaluated using the Y-maze test and positron emission tomography with fluorine-18 fluorodeoxyglucose, respectively. tMCAO led to cognitive impairment, which was improved by TH. The cerebral glucose metabolism of the thalamus was significantly recovered in the TH group compared to that in the tMCAO group. These results suggest that TH might have potential as a therapeutic strategy for improving the ischemia/reperfusion-induced cognitive impairment.

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Isoflurane has no effect on cognitive or behavioral performance in a mouse model of early-stage Alzheimer’s disease

Cited by 5 publications

References 41 publications

Genotype- and sex-specific changes in vital parameters during isoflurane anesthesia in a mouse model of Alzheimer’s disease

Genotype- and sex-specific changes in vital parameters during isoflurane anesthesia in a mouse model of Alzheimer’s disease

Effect of Therapeutic Hypothermia on Cognitive Impairment in a Mouse Model of Ischemia-Reperfusion Injury

Neuroprotective Effect of Therapeutic Hypothermia in Cognitive Impairment of tMCAo Mouse Model

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