Isoflurane: An Ideal Anesthetic for Rodent Orthotopic Liver Transplantation Surgery?

Cao, Ding; Liu, Y.; Li, J.; Gong, Jianping

doi:10.1016/j.transproceed.2016.06.057

Cited by 3 publications

(4 citation statements)

References 15 publications

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“…Continuous suture and cuff method comprise the reconstruction of SHVC [1.2.9-14], suturing should be completed rapidly while SHVC is blocked and it necessitates the diaphragmatic ring being clamped a little more without ventilator aid. The diaphragm is to maintain respiratory movement, once clamped and retracted downward (caudalad), it dramatically affects respiratory and causes the rat to move due to compromised respiration (supplemental video 1), some surgeons might add anesthesia, resultantly the rat will die, especially under plain and simple mask anesthesia inhalation (ether etc), this is the reason that cardiac arrest and higher mortality occurred during AHT [7]. Cuff method e ciently shortens SHVC anastomosis, but it is not universally applicable due to short SHVC in length.…”

Section: Discussionmentioning

confidence: 99%

“…Rat OLT is well-accepted model especially in the research of tolerance induction since Lee and his colleagues developed [1,2], it is complicated in microsurgical techniques, especially for new microsurgeons although many valuable modi cations have come into forth [3][4][5]. It is documented that the short anhepatic time plays a critical role of the success of LT and improves survival greatly, and that cardiac arrest in the recipient frequently occurred when AHT was more than 26 minutes [6,7]. Higher mortality rate during AHT necessitates researchers to extend AHT.…”

Section: Introductionmentioning

confidence: 99%

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The simple maneuver to safely extend the anhepatic time to 30 minutes for rat liver transplantation

Tang

Zhang

Zhao

et al. 2021

Preprint

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Background Orthotopic rat liver transplantation (OLT) is widely used; it remains to be challenging although many renovations have been made. Methods For tolerance induction, we performed OLT including whole graft OLT from close Sprague Dawley (SD) rat to SD (whole graft group, control group, n = 21) and 30 minute anhepatic time (AHT) group (AHT group, n = 11). For good outcomes of AHT group and generalization of this maneuver, partial OLT was performed from 50% Lewis allograft to Brown Norway (BN) rats to induce tolerance (half graft group, n = 28), Cyclosporine A was injected once daily for 14 days. Results For whole graft group, 30-day survival rate was 85.5% (18/21), the reasons of death were gas embolism due to the missed suturing in 2 cases, blood loss in 1 case. For AHT group and tolerance group, 30-day survival rate was 72.7% (8/11), 64.3% (18/28), the causes of death were diverse. There were no differences in survival in 3 groups (p = 0.289). Conclusion The anhepatic time can be extended simply through the change of clamping the diaphragm, which facilitates its application in the research.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The simple maneuver to safely extend the anhepatic time to 30 minutes for rat liver transplantation

Tang

Zhang

Zhao

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…Continuous suture and cuff method comprise the reconnection of SHVC [1.2.9-14], suturing should be completed rapidly while SHVC is blocked and it necessitates clamping the diaphragmatic ring a little more without ventilator assisting. The diaphragm is to maintain respiratory movement, once clamped, that dramatically affects respiratory and causes rats to move due to compromised respiration (supplemental video 1), some surgeons might add anesthesia, consequently the rats will die, especially under plain and simple mask anesthesia inhalation (ether etc), this is the reason that cardiac arrest occurred and higher mortality came forth during AHT [7]. Cuff method e ciently shortens SHVC anastomosis, but it is not universally applicable due to short SHVC in length.…”

Section: Discussionmentioning

confidence: 99%

“…Rat OLT is well-accepted model on immunology, ischemia-reperfusion injury and regeneration since Lee and his colleagues developed [1,2], nowadays it is complicated requiring microsurgical techniques, especially for new microsurgeons although many valuable modi cations have come into use [3][4][5]. It is documented that the short anhepatic time plays a critical role of the success of LT and improves survival greatly, and that cardiac arrest in the recipient frequently occurred when AHT was more than 26 minutes [6,7]. Higher mortality rate during AHT necessitates researchers to extend AHT.…”

Section: Introductionmentioning

confidence: 99%

The simple surgery to safely extend the anhepatic time to 30 minutes for rat liver transplantation

Tang

Zhang

Zhao

et al. 2020

Preprint

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Abstract Background Orthotopic rat liver transplantation (OLT) is widely used, it remains complicated although many renovations have been made. Methods OLT was performed for tolerance induction, including whole graft OLTs from close male Sprague Dawley (SD) rat to male SD (whole graft group, n = 21) and 30 minute anhepatic time group (AHT group, n = 11), OLT was performed from 50% male Lewis allograft to male Brown Norway (BN) rats to induce tolerance (half graft group, n = 28), Cyclosporine A was injected once daily for 14 days. Results For whole graft group, 30-day survival rate was 85.5% (18/21), the reasons of death were gas embolism due to the missed suturing in 2 cases, blood loss in 1 case. For AHT group, 30-day survival rate 72.7% (8/11), the causes of death were respiratory failure in 1 case, unknown in 2 cases. For tolerance group, 30-day survival rate was 64.3% (18/28), the reasons of death were diarrhea in 4 cases, wound dehiscence in 6 cases. There were no differences in survival in 3 groups (p = 0.289). Conclusion The anhepatic time can be extended simply through the change of clamping the diaphragm, which facilitates its application in the research.

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Circulating levels of miR-122 increase post-mortem, particularly following lethal dosing with pentobarbital sodium: implications for pre-clinical liver injury studies

Clarke

Forootan

Lea

et al. 2017

Toxicology Research

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microRNA-122 (miR-122) is increasingly being measured in pre-clinical and clinical settings due to greater sensitivity and hepatic specificity compared to the gold standard liver injury biomarker alanine aminotransferase (ALT). In pre-clinical studies, various culling methods can be employed prior to collection of blood samples, including lethal injection with pentobarbital sodium (Pentoject). However, little is known about whether such an approach could alter the circulating levels of miR-122 and compromise the interpretation of data. We therefore exposed C57BL/6J mice to saline or the model hepatotoxin paracetamol and collected blood samples pre-cull ( tail bleed) and post-cull ( cardiac puncture following exposure to a rising concentration of CO or intraperitoneal injection of Pentoject). Compared to pre-cull levels there was a significant increase in serum miR-122 level in mice culled with CO and, to a much greater extent, in mice culled with Pentoject. As a result, whilst the serum level of miR-122 increased in Pentoject-culled animals exposed to paracetamol, the higher level in saline-treated mice rendered this difference statistically non-significant, in contrast to findings in animals culled with CO. ALT levels were unaffected by sacrifice method. Consistent with the findings, exposure of primary mouse hepatocytes to Pentoject provoked a rapid and concentration-dependent release of miR-122 into the culture media. Thus, for optimal design and interpretation of data from pre-clinical liver injury studies in which miR-122 is to be used as a biomarker, we recommend that blood samples are collected pre-cull whenever possible, and that lethal injection with Pentoject is avoided.

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Isoflurane: An Ideal Anesthetic for Rodent Orthotopic Liver Transplantation Surgery?

Cited by 3 publications

References 15 publications

The simple maneuver to safely extend the anhepatic time to 30 minutes for rat liver transplantation

The simple maneuver to safely extend the anhepatic time to 30 minutes for rat liver transplantation

The simple surgery to safely extend the anhepatic time to 30 minutes for rat liver transplantation

Circulating levels of miR-122 increase post-mortem, particularly following lethal dosing with pentobarbital sodium: implications for pre-clinical liver injury studies

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