Isoflavonoids from the Roots ofCampylotropis hirtella

Abstract

From the dried roots of Campylotropis hirtella (Franch.) Schindl., five novel isoflavonoids, 3( S)-7,2',4'-trihydroxy-5,5'-dimethoxy-6-(3-methylbut-2-enyl)-isoflavan ( 1), 3( S)-2',4'-dihydroxy-5,5'-dimethoxy-(6'',6''-dimethylpyrano)-(2'',3'':7,6)-isoflavan ( 2), 3( R)-5,4'-dihydroxy-2'-methoxy-3'-(3-methylbut-2-enyl)-(6'',6''-dimethylpyrano)-(7,6 : 2'',3'')-isoflavanone ( 3), 3( R)-5,4'-dihydroxy-2'-methoxy-(6'',6''-dimethylpyrano)-(7,6: 2'',3'')-isoflavanone ( 4), and 3( R)-5,4'-dihydroxy-7,2'-dimethoxy-6-geranylisoflavanone ( 5) were isolated. The structures of the compounds were elucidated on the basis of spectroscopic analysis. All isolates exhibited good immunosuppressive activities on mitogen-induced splenocyte proliferation, and their cytotoxicity on splenic lymphocytes was also tested.

Human neutrophil elastase inhibitory potential of flavonoids fromCampylotropis hirtellaand their kinetics

“…0 -geranyl-5,7,2 0 ,5 0 -tetrahy-droxyisoflavone (5), myrsininone A (6), isosophoranone (7), and myrsininone B (8) through analysis of spectroscopic data and by comparison with previous studies [22][23][24] . The exact chemical structures were fully determined from spectroscopic data including 2D-NMR (Figure 1 Human neutrophil esterase inhibitory activity HNE is controlled by its endogenous inhibitors, like a1-antitrysin, but the affinity of these endogenous inhibitors for HNE is strongly decreased by oxidative stress and by proteases released upon inflammation.…”

A new potent immunosuppressive isoflavanonol from Campylotropis hirtella

“…Previous chemical studies revealed that the plant C. hirtella contains tannins (Qin et al 1991), steroids, triterpenes (Lu et al 1997), flavonoids , coumarins , lignans , C-glycosylfavones and aromatic glycosides (Wen et al 2007). More than 50 new flavonoids were isolated from C. hirtella in our laboratory (Shou, Fu, et al 2009;Shou et al 2009a;Shou et al 2009b;Zhang et al 2010;Tan et al 2011;Li et al 2014), and some of these flavonoids were found to possess very interesting biological activities. For example, Hirtellanine A ( Figure 1) showed significant inhibitory activity against B lymphocyte proliferation.…”

Flavonoids from the Roots ofCampylotropis hirtella

“…MTT (5 mg/mL) reagent was added 4 h before the end of culture, the supernatants discarded, and the cells lysed with DMSO. The OD 570 values of the samples were obtained, and the percentage of cell death was calculated [12].…”

“…Its dried roots are used as a traditional Chinese medicine for the treatment of irregular menstruation, dysmenorrhea, metrorrhagia, and metrostaxis as well as gastric ulcers [2]. Various chemical entities including tannins, steroids, triterpenes, flavonoids, coumarins, lignans, C-glycosylfavones, and aromatic glycosides have been isolated from this plant [3][4][5][6][7][8][9][10][11][12][13]. Our previous study revealed that a coumaronochromone from this plant possesses very potent immunosuppressive activity [11] and, as a continuation of our work identifying promising immunosuppressants from this Chinese medical material, eight new flavonoids were isolated from the dried roots of C. hirtella (three pterocarpenes, 1, 2, 3; two flavanones, 4, 5; and three isoflavones, 6,7,8).…”