Isoflavonoids are present in Arabidopsis thaliana despite the absence of any homologue to known isoflavonoid synthases

Lapčı́k, Oldřich; Honys, David; Koblovská, R.; Mackova, Zuzana; Vítková, Michaela; Klejdus, Bořivoj

doi:10.1016/j.plaphy.2005.11.006

Cited by 21 publications

(13 citation statements)

References 26 publications

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“…Separately, isoflavone, which is prevalent in legumes, is found to exist in A. thaliana although A. thaliana does not possess the genetic machinery (annotation of genome) of isoflavone synthesis. 22) Hence, we cannot exclude the possibility that glucosinolates are biosynthesized in V. faba in response to certain stress or that endogenous ITCs induce stomatal closure in V. faba.…”

Section: Discussionmentioning

confidence: 99%

Allyl isothiocyanate induces stomatal closure in Vicia faba

Sobahan

Akter

Okuma

et al. 2015

Bioscience, Biotechnology, and Biochemistry

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Isothiocyanates are enzymatically produced from glucosinolates in plants, and allyl isothiocyanate (AITC) induces stomatal closure in Arabidopsis thaliana. In this study, we investigated stomatal responses to AITC in Vicia faba. AITC-induced stomatal closure accompanied by reactive oxygen species (ROS) and NO production, cytosolic alkalization and glutathione (GSH) depletion in V. faba. GSH monoethyl ester induced stomatal reopening and suppressed AITC-induced GSH depletion in guard cells. Exogenous catalase and a peroxidase inhibitor, salicylhydroxamic acid, inhibited AITC-induced stomatal closure, unlike an NAD(P)H oxidase inhibitor, diphenylene iodonium chloride. The peroxidase inhibitor also abolished the AITC-induced ROS production, NO production, and cytosolic alkalization. AITC-induced stomatal closure was suppressed by an NO scavenger, 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide, and an agent to acidify cytosol, butyrate. These results indicate that AITC-induced stomatal closure in V. faba as well as in A. thaliana and suggest that AITC signaling in guard cells is conserved in both plants.

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Section: Discussionmentioning

confidence: 99%

Allyl isothiocyanate induces stomatal closure in Vicia faba

Sobahan

Akter

Okuma

et al. 2015

Bioscience, Biotechnology, and Biochemistry

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“…Finally, analyses on isoflavones such as daidzein and genistein are potentially useful for identification and separation of varieties [ 49 , 61 ]. As an example of more detailed investigations on isoflavones see Lapcik et al [ 62 ], who used five isoflavone specific enzyme-linked immunosorbent assays (ELISAs) after HPLC sample fractionation to identify isoflavones in Arabidopsis .…”

Section: Future Perspectivesmentioning

confidence: 99%

A Guide to the Variability of Flavonoids in Brassica oleracea

2017

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Flavonoids represent a typical secondary metabolite class present in cruciferous vegetables. Their potential as natural antioxidants has raised considerable scientific interest. Impacts on the human body after food consumption as well as their effect as pharmaceutical supplements are therefore under investigation. Their numerous physiological functions make them a promising tool for breeding purposes. General methods for flavonoid analysis are well established, though new compounds are still being identified. However, differences in environmental circumstances of the studies and analytical methods impede comparability of quantification results. To promote future investigations on flavonoids in cruciferous plants we provide a checklist on best-practice in flavonoid research and specific flavonoid derivatives that are valuable targets for further research, choosing a representative species of scientific interest, Brassica oleracea.

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“…In addition, Lapcik et al (2006) found that isoflavonoids are present in A. thaliana despite the absence of any homologue to known isoflavonoid synthase and they concluded that another gene must be responsible for the biosynthesis of the isoflavone skeleton in Brassicaceae.…”

Section: Isoflavone Synthase and Isoflavone Reductasementioning

confidence: 99%

An in silico analysis of the key genes involved in flavonoid biosynthesis in Citrus sinensis

et al. 2007

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Citrus species are known by their high content of phenolic compounds, including a wide range of flavonoids. In plants, these compounds are involved in protection against biotic and abiotic stresses, cell structure, UV protection, attraction of pollinators and seed dispersal. In humans, flavonoid consumption has been related to increasing overall health and fighting some important diseases. The goals of this study were to identify expressed sequence tags (EST) in Citrus sinensis (L.) Osbeck corresponding to genes involved in general phenylpropanoid biosynthesis and the key genes involved in the main flavonoids pathways (flavanones, flavones, flavonols, leucoanthocyanidins, anthocyanins and isoflavonoids). A thorough analysis of all related putative genes from the Citrus EST (CitEST) database revealed several interesting aspects associated to these pathways and brought novel information with promising usefulness for both basic and biotechnological applications

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Isoflavonoids are present in Arabidopsis thaliana despite the absence of any homologue to known isoflavonoid synthases

Cited by 21 publications

References 26 publications

Allyl isothiocyanate induces stomatal closure in Vicia faba

Allyl isothiocyanate induces stomatal closure in Vicia faba

A Guide to the Variability of Flavonoids in Brassica oleracea

An in silico analysis of the key genes involved in flavonoid biosynthesis in Citrus sinensis

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