Isoflavonoides Da Tribo Dalbergieae: Uma Contribuição Quimiossistemática Para a Subfamília Papilionoideae

Carvalho, Adonias Almeida; Santos, Lucivania Rodrigues dos; Freitas, Jurema Santana de; Chaves, Mariana Helena

doi:10.21577/0100-4042.20170588

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“…The literature reports the compounds identified in this study in species of the Fabaceae family, tribe Dalbergieae, which suggests that the bees collected the resins from species of the genus Dalbergia [ 35 , 40 ]. A phytogeographic study was conducted near the area where the propolis used in this study was collected, and identified the presence of the plant species Dalbergia monetaria L.f. (Fabaceae) and D. ecastaphyllum , which corroborates our results [ 41 ].…”

Section: Discussionmentioning

confidence: 95%

Brazilian Amazon Red Propolis: Leishmanicidal Activity and Chemical Composition of a New Variety of Red Propolis

Dutra,

de Sousa,

Mignoni

et al. 2023

Metabolites

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Leishmaniasis is caused by protozoans of the genus Leishmania, and its treatment is highly toxic, leading to treatment discontinuation and the emergence of resistant strains. In this study, we assessed the leishmanicidal activity and chemical composition of red propolis collected from the Amazon-dominated region of northern Tocantins State, Brazil. The MTT assay was employed to determine the samples’ activity against Leishmania amazonensis promastigotes and their cytotoxicity against RAW macrophages. Spectrophotometric assays were utilised to measure the concentrations of total phenolics and flavonoids, while high-performance liquid chromatography coupled to a mass spectrometer (LC-MS/MS) was used to determine the chemical composition. An in silico study was conducted to evaluate which compounds from Brazilian Amazon red propolis may correlate with this biological activity. Brazilian Amazon red propolis exhibited a high concentration of phenolic compounds and an inhibitory activity against L. amazonensis, with an IC50 ranging from 23.37 to 36.10 µg/mL. Moreover, fractionation of the propolis yielded a fraction with enhanced bioactivity (16.11 µg/mL). Interestingly, neither the propolis nor its most active fraction showed cytotoxicity towards macrophages at concentrations up to 200 µg/mL. The red colour and the presence of isoflavonoid components (isoflavones, isoflavans, and pterocarpans) confirm that the substance is Brazilian red propolis. However, the absence of polyprenylated benzophenones suggests that this is a new variety of Brazilian red propolis. The in silico study performed with two of the main leishmanicidal drug targets using all compounds identified in Amazon red propolis reported that liquiritigenin was the compound that exhibited the best electronic interaction parameters, which was confirmed in an assay with promastigotes using a standard. The findings indicate that Amazon red propolis possesses leishmanicidal activity, low toxicity, and significant biotechnological potential.

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Section: Discussionmentioning

confidence: 95%