Isoflavonoid production by soy plant callus suspension culture

Gueven, Alper; Knorr, Dietrich

doi:10.1016/j.jfoodeng.2010.10.019

Cited by 66 publications

(33 citation statements)

References 25 publications

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“…150 μM of the elicitor concentration and 72h exposure time verbascoside level (8.45 mg·g -1 DW) was only 1.4-fold higher in comparison to the control (6.04 mg·g -1 DW) and up to 5.8 times lower than that in the roots treated with the same concentration of MeJa at the same exposure time. Likewise, Gueven and Knorr (2011) reported that MeJa was more effective than SA for production of isoflavones in soybean cell suspension culture.…”

Section: Results and Discussion Growth Kinetics In Shake Flasksmentioning

confidence: 99%

The Influence of Methyl Jasmonate and Salicylic Acid on Secondary Metabolite Production in Rehmannia Glutinosa Libosch. Hairy Root Culture

Piątczak¹,

Kuźma²,

Wysokińska³

2016

Acta Biologica Cracoviensia S. Botanica

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Rehmannia glutinosa hairy roots were used to evaluate the effect of methyl jasmonate (MeJa) and salicylic acid (SA) on increase of root biomass and production of iridoids (catalpol, harpagide) and phenylethanoids (verbascoside and isoverbascoside). The elicitors were added to 23-day-old culture separately at concentrations between 50 and 200 μM or in combinations at concentrations of 50 and 100 μM. Roots were harvested 72 h and 120 h after elicitation. The type of elicitor, its concentration and exposure time were found to strongly affect the content of each analyzed compound. A 72-hour treatment with 200 μM MeJa was the most effective in increase of verbascoside content (60.07 mg·DW -1 equivalent to 845.45 mg·L -1 ) and isoverbascoside (1.77 mg·DW -1 equivalent to 24.94 mg·L -1 ): these respective amounts were roughly 10-and 6.4-fold higher than the control values (unelicited roots). Exposure to 150 μM MeJa provided optimal harpagide content after 72 hours (0.136 mg·DW -1 ; 7.5-fold increase compared to the control), and catalpol content after 120 hours (up to 2.145 mg·DW -1 ). The combination of MeJa and SA also resulted in higher levels of secondary metabolites compared to the control culture, although these levels were lower than those observed for MeJa alone at the optimal concentration and exposure time. SA alone was less efficient in enhancing metabolite production than MeJa.

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Section: Results and Discussion Growth Kinetics In Shake Flasksmentioning

confidence: 99%

The Influence of Methyl Jasmonate and Salicylic Acid on Secondary Metabolite Production in Rehmannia Glutinosa Libosch. Hairy Root Culture

Piątczak¹,

Kuźma²,

Wysokińska³

2016

Acta Biologica Cracoviensia S. Botanica

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“…Although there has been an increasing interest in isoflavonoid production from plant tissue grown in vitro from soy, 11) P. lobata 10) and P. mirifica, 46) so as to make the production independent of the influence of variation from changes in the physical environment, this in vitro approach is often more expensive than a conventional large scale production method using farm grown plants, which for example, can produce an abundant mass of leaves.…”

Section: Yes Assaymentioning

confidence: 99%

“…9) However, a new source of isoflavonoids would promote the alternative production and consumption of such chemicals from those new plant sources and relieve the pressure on the currently limited amount of available plant material and risks associated with sole large scale monoculture production 10) or the in vitro culture of plants. 11) Pueraria mirifica Airy Shaw et Suvatabhandu is a Thai indigenous legume herb with a long-term folk medicinal consumption among Thai women for menopausal treatment. The tuberous materials of the plant revealed strong estrogenic effects in the MCF-7 proliferation/antiproliferation, 12) uterotrophic 13,14) and Yeast Estrogen Screen (YES) 15) test assays.…”

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confidence: 99%

Pueraria mirifica leaves, an alternative potential isoflavonoid source

Jungsukcharoen

Dhiani

Cherdshewasart

et al. 2014

Bioscience, Biotechnology, and Biochemistry

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We investigated the major leaf isoflavonoid contents of Pueraria mirifica from three different cultivars (PM-III, PM-IV, and PM-V) using reverse RP-HPLC analysis. The proportions and net levels of puerarin, daidzin, genistin, and daidzein in P. mirifica leaves were found to depend on the plant cultivar and to correlate with cultivation temperature and rainfall amount. The crude leaf-extracts were tested using the Yeast Estrogen Screen (YES) assay with both human estrogen receptors (hERα and hERβ). Their estrogenic activity was higher when determined by the YES system containing hERβ than that with hERα and was also higher when the Δsnq2 than the wildtype yeast was employed. The results open the possibility of selecting and cultivating certain P. mirifica cultivars at a farm scale to produce a sufficient supply of leaf material to act as a starting source for the commercial scale extraction of these major isoflavonoids.

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“…Methyl jasmonate also elicited by 5-6 times the production of isofl avonoids by Glycine max plant cell suspension batch culture (Gueven and Knorr 2010 ).…”

Section: Elicitationmentioning

confidence: 99%

“…The accumulation of secondary metabolites can be according two different patterns related to plant growth and development: (a) they accumulate during the stationary growth phase or associated to differentiation, as is the case of the anthocyanines produced by V. vinifera cultures; (b) accumulation during the exponential growth phase, as is the case of isofl avonoids by soy plant callus suspension culture (Gueven and Knorr 2010 ). The second case is the most frequent because plants need a certain degree of differentiation for the synthesis of determined secondary metabolites.…”

Section: Differentiation and Compartmentationmentioning

confidence: 99%

In Vitro Plant Cultures as Biofactories

Álvarez

2014

Plant Biotechnology for Health

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In vitro plant cell cultures are defi ned as a culture in aseptic conditions of any part of the plant body in a nutritive media. Cells, differentiated and undifferentiated (calli and suspended cells) tissues, and organs can be maintained in vitro .Some of the advantages of in vitro cultures are their development and maintenance in defi ned conditions (nutrients, light, humidity, temperature), independent from environmental infl uences (pests, diseases, extreme weather, geographical location, etc.), and manipulated by experienced operators in confi ned facilities.Since its development, in vitro plant cell cultures have been an essential tool for studying plant metabolism, and to micropropagate plant species, maintaining their genetic background. Also, they were helpful to analyze and optimize the production of plant secondary metabolites.Secondary metabolites productive processes are conducted, with a different degree of success, in undifferentiated cultures and in cultures of transformed organs. Several strategies were displayed in order to increase yields as manipulation of the plant growth regulator balance, addition of precursors, biotransformation, and immobilization.Also, as plant cells have a different behavior in liquid culture compared with microorganisms and mammal cells, bioreactor design was adapted to develop plant cell cultures.

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Isoflavonoid production by soy plant callus suspension culture

Cited by 66 publications

References 25 publications

The Influence of Methyl Jasmonate and Salicylic Acid on Secondary Metabolite Production in Rehmannia Glutinosa Libosch. Hairy Root Culture

The Influence of Methyl Jasmonate and Salicylic Acid on Secondary Metabolite Production in Rehmannia Glutinosa Libosch. Hairy Root Culture

Pueraria mirifica leaves, an alternative potential isoflavonoid source

In Vitro Plant Cultures as Biofactories

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