Isoflavones derived from plant raw materials: bioavailability, anti-cancer, anti-aging potentials, and microbiome modulation

Aboushanab, Saied A.; Khedr, Shaimaa M; Гетте, И. Ф.; Данилова, И. Г.; Кольберг, Наталья Александровна; Ravishankar, G. A.; Ambati, Ranga Rao; Kovaleva, Elena G.

doi:10.1080/10408398.2021.1946006

Cited by 42 publications

(25 citation statements)

References 269 publications

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“…Seven Ac-Glu isomers (m/z 459, 489 and 475 [M + H] + , based on D, Gy and Gn, respectively) could be tentatively characterized as ' After steaming, Ac-Glu slightly contained in raw soybeans increased potentially as numerous isomers due to decarboxylation of Mal-Glu [32]. Seven Ac-Glu isomers (m/z (1) New isoflavone derivatives in this source. (2) Further confirmed in comparison with authentic standards.…”

Section: Identification Of 38 Isoflavone Derivatives In Raw Steamed A...mentioning

confidence: 98%

“…ND, not detected. (1) New isoflavone derivatives in this source. (2) Further confirmed in comparison with authentic standards.…”

Section: Succinyl-glucosides and Phosphorylated Conjugates During Fer...mentioning

confidence: 99%

“…Isoflavones are mainly distributed as O-glycosyl or C-glycosyl derivatives with acylated moieties (e.g., none-, acetyl-and malonyl-) in soybean, kudzu, astragalus and red clover belonging to Leguminosae Family [1,2] and have been reported to have potential preventive effects in a wide range of human diseases such as cancer, hypertension, atherosclerosis, diabetes, hyperlipidemia, menopausal symptoms, osteoporosis, neurodegeneration and multiple sclerosis [1,3,4]. Particularly, soybean (Glycine max L.), commonly known as an isoflavone-rich source, is one of the most important crops consumed worldwide because it provides the functional nutrients (vegetable protein, oil, fatty acid, isoflavone, saponin, etc.)…”

Section: Introductionmentioning

confidence: 99%

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Changes in Isoflavone Profile from Soybean Seeds during Cheonggukjang Fermentation Based on High-Resolution UPLC-DAD-QToF/MS: New Succinylated and Phosphorylated Conjugates

et al. 2022

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In this study, thirty-eight isoflavone derivatives were comprehensively identified and quantified from the raw, steamed and fermented seeds of four selected soybean cultivars based on UPLC-DAD-QToF/MS results with reference to the previously reported LC-MS library and flavonoid database, and summarized by acylated group including glucosides (Glu), malonyl-glucosides (Mal-Glu), acetyl-glucosides (Ac-Glu), succinyl-glucosides (Suc-Glu) and phosphorylated conjugates (Phos) in addition to aglycones. Among them, Suc-Glu and Phos derivatives were newly generated due to fermentation by B. subtilis AFY-2 (cheonggukjang). In particular, Phos were characterized for the first time in fermented soy products using Bacillus species. From a proposed roadmap on isoflavone-based biotransformation, predominant Mal-Glu (77.5–84.2%, raw) decreased rapidly by decarboxylation and deesterification into Ac-Glu and Glu (3.5–8.1% and 50.0–72.2%) during steaming, respectively. As fermentation continued, the increased Glu were mainly succinylated and phosphorylated as well as gradually hydrolyzed into their corresponding aglycones. Thus, Suc-Glu and Phos (17.3–22.4% and 1.5–5.4%, 36 h) determined depending on cultivar type and incubation time, and can be considered as important biomarkers generated during cheonggukjang fermentation. Additionally, the changes of isoflavone profile can be used as a fundamental report in applied microbial science as well as bioavailability research from fermented soy foods.

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Section: Identification Of 38 Isoflavone Derivatives In Raw Steamed A...mentioning

confidence: 98%

“…ND, not detected. (1) New isoflavone derivatives in this source. (2) Further confirmed in comparison with authentic standards.…”

Section: Succinyl-glucosides and Phosphorylated Conjugates During Fer...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Changes in Isoflavone Profile from Soybean Seeds during Cheonggukjang Fermentation Based on High-Resolution UPLC-DAD-QToF/MS: New Succinylated and Phosphorylated Conjugates

et al. 2022

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“…Soy isoflavones have obvious therapeutic effects on breast cancer, colon cancer, lung cancer, prostate cancer, skin cancer, and leukemia. Soy isoflavones can also prevent the occurrence of ovarian cancer, colon cancer, stomach cancer, and prostate cancer ( Suthar et al, 2001 ; Sarkar et al, 2006 ; Kaushik et al, 2018 ; Park et al, 2018 ; Imai-Sumida et al, 2020 ; Aboushanab et al, 2021 ). In addition, down-regulation of ALKBH5 expression enhanced the expression of mesenchymal phenotype markers α-smooth muscle actin and snail, which are factors that promote EMT.…”

Section: Part 3: the Anti-cancer Effect Of Nature Products By Regulat...mentioning

confidence: 99%

The Role of m6A RNA Methylation in Cancer: Implication for Nature Products Anti-Cancer Research

et al. 2022

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N6-methyladenosine (m6A) RNA methylation is identified as the most common, abundant and reversible RNA epigenetic modification in messenger RNA (mRNA) and non-coding RNA, especially within eukaryotic messenger RNAs (mRNAs), which post-transcriptionally directs many important processes of RNA. It has also been demonstrated that m6A modification plays a pivotal role in the occurrence and development of tumors by regulating RNA splicing, localization, translation, stabilization and decay. Growing number of studies have indicated that natural products have outstanding anti-cancer effects of their unique advantages of high efficiency and minimal side effects. However, at present, there are very few research articles to study and explore the relationship between natural products and m6A RNA modification in tumorigenesis. m6A is dynamically deposited, removed, and recognized by m6A methyltransferases (METTL3/14, METTL16, WTAP, RBM15/15B, VIRMA, CBLL1, and ZC3H13, called as “writers”), demethylases (FTO and ALKBH5, called as “erasers”), and m6A-specific binding proteins (YTHDF1/2/3, YTHDC1/2, IGH2BP1/2/3, hnRNPs, eIF3, and FMR1, called as “readers”), respectively. In this review, we summarize the biological function of m6A modification, the role of m6A and the related signaling pathway in cancer, such as AKT, NF-kB, MAPK, ERK, Wnt/β-catenin, STAT, p53, Notch signaling pathway, and so on. Furthermore, we reviewed the current research on nature products in anti-tumor, and further to get a better understanding of the anti-tumor mechanism, thus provide an implication for nature products with anti-cancer research by regulating m6A modification in the future.

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“…The pharmaceutical potency of plant-derived compounds is remarkably dependent on their dose, chemical profile, and bioavailability [ 19 ]. Hence, it is essential to determine the unique profile and quantify the plant-derived isoflavones to assess therapeutic interventions in vitro and in vivo.…”

Section: Introductionmentioning

confidence: 99%

Antioxidant and Cytotoxic Activities of Kudzu Roots and Soy Molasses against Pediatric Tumors and Phytochemical Analysis of Isoflavones Using HPLC-DAD-ESI-HRMS

Aboushanab

Шевырин

Slesarev

et al. 2022

Plants

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Pediatric solid tumors (PSTs) are life-threatening and can lead to high morbidity and mortality rates in children. Developing novel remedies to treat these tumors, such as glioblastoma multiforme and sarcomas, such as osteosarcoma, and rhabdomyosarcoma, is challenging, despite immense attempts with chemotherapeutic or radiotherapeutic interventions. Soy (Glycine max) and kudzu roots (KR) (Pueraria spp.) are well-known phytoestrogenic botanical sources that contain high amounts of naturally occurring isoflavones. In the present study, we investigated the antioxidant and cytotoxic effects of the extracts of KR and soy molasses (SM) against PSTs. The green extraction of isoflavones from KR and SM was performed using natural deep eutectic solvents. The extracts were subsequently analyzed by high-performance liquid chromatography (HPLC)-diode array detector (DAD) coupled with high-resolution (HR) mass spectrometry (MS), which identified 10 isoflavones in KR extracts and 3 isoflavones in the SM extracts. Antioxidant and cytotoxic activities of KR and SM extracts were assessed against glioblastoma multiforme (A-172), osteosarcoma (HOS), and rhabdomyosarcoma (Rd) cancer cell lines. The KR and SM extracts showed satisfactory cytotoxic effects (IC50) against the cancer cell lines tested, particularly against Rd cancer cell lines, in a dose-dependent manner. Antioxidant activity was found to be significantly (p ≤ 0.05) higher in KR than in SM, which was consistent with the results of the cytotoxic activity observed with KR and SM extracts against glioblastoma and osteosarcoma cells. The total flavonoid content and antioxidant activities of the extracts were remarkably attributed to the isoflavone content in the KR and SM extracts. This study provides experimental evidence that HPLC-ESI-HRMS is a suitable analytical approach to identify isoflavones that exhibit potent antioxidant and anticancer potential against tumor cells, and that KR and SM, containing many isoflavones, can be a potential alternative for health care in the food and pharmaceutical industries.

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Isoflavones derived from plant raw materials: bioavailability, anti-cancer, anti-aging potentials, and microbiome modulation

Cited by 42 publications

References 269 publications

Changes in Isoflavone Profile from Soybean Seeds during Cheonggukjang Fermentation Based on High-Resolution UPLC-DAD-QToF/MS: New Succinylated and Phosphorylated Conjugates

Changes in Isoflavone Profile from Soybean Seeds during Cheonggukjang Fermentation Based on High-Resolution UPLC-DAD-QToF/MS: New Succinylated and Phosphorylated Conjugates

The Role of m6A RNA Methylation in Cancer: Implication for Nature Products Anti-Cancer Research

Antioxidant and Cytotoxic Activities of Kudzu Roots and Soy Molasses against Pediatric Tumors and Phytochemical Analysis of Isoflavones Using HPLC-DAD-ESI-HRMS

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