Isoflavone profiles and antioxidant properties in different parts of soybean sprout

Abstract

Sprouting is one of the processing methods to enhance bioavailability of some nutrients of agricultural products. Soybeans were germinated for 6 days and soybean (SB), soybean sprout (SBS), cotyledon of soybean sprout (CSS), and hypocotyl and root of soybean sprout (HRSS) were collected. Isoflavone profiles and in vitro antioxidant activities of 80% ethanol extracts of samples were analyzed. In addition, oxidative stability of the extract was evaluated in corn oils heated at 100 °C. The hypocotyl and root part of soybean sprout contained significantly (P < 0.05) higher content of isoflavone aglycones than the cotyledon part of soybean sprout. Diphenyl‐1‐picrylhydrazyl radial and 2,2’‐azinobis(3‐ethylbenzothiazoline‐6‐sul‐fonic acid cation radical scavenging activities of SBS were 1.6 and 1.3 times higher than those of SB, respectively (P < 0.05), whereas those of CSS were 1.1 and 1.8 times higher than those of HRSS, respectively (P < 0.05). CSS had higher ORAC and FRAP values than others. High correlations between contents of total flavonoids and antioxidant activities were observed in all in vitro antioxidant assays. SBS, CSS, and HRSS had significantly higher oxidative stability than SB in 100 °C heated corn oil after 9 hr treatment (P < 0.05). Generally, 80% ethanol extract of CSS had high in vitro antioxidant properties whereas HRSS had high aglycone forms of isoflavones. Isoflavone aglycones may not be major ingredients providing antioxidant activities of soybean sprout. Practical Application Soybeans are important crops for the preparation of tofu, soymilk, and protein sources. Soybean sprout prepared by germinating soybean and growing for several days is one of important food materials used as daily diet in some countries. In this study, antioxidant activities of different parts of soybean sprouts were evaluated using in vitro methods and bulk oil model systems. In addition, isoflavone profiles of soybean sprouts were determined. Enhanced antioxidant activities of soybean sprouts may help consumers choose healthier foods and extend business areas of the soybean food industry.

SR-5, the specific ratio of Korean multi-herbal formula: An evaluation of antiulcerogenic effects on experimentally induced gastric ulcers in mice

“…62-64 It has also been reported that soybean sprouts, the main component of SR-5, have higher in vitro antioxidant activities than soybean and soybean oil. 16,17 The present study showed that pretreatment with SR-5 significantly protected the gastric mucosa from HCl/EtOH- and INDO/HCl-induced ulceration by restoring the depleted GSH level and CAT activity, together with reducing the level of MDA and ROS. Thus, the protective effect of SR-5 against ulcers could be partly attributed to its inhibitory effect on oxidative stress and lipid peroxidation.…”

“…Based on ethnopharmacological information, our previous hepatoprotective experiments, 15 and the reported antioxidant and anti-inflammatory effects and safety data, we selected 12 Korean medicinal herbs: soybean sprouts, 16,17 Oenanthe javanica (Blume) DC., 18 immature Citrus reticulata Blanco peel, 19 Hovenia dulcis Thunb., 20,21 immature Rubus coreanus Miq., 22,23 Artemisia capillaris Thunb., 24 Chaenomeles sinensis (Dum. Cours.)…”

“…35 The effects of germination on the antioxidant activities of soybean sprouts have been reported in diverse conditions 16 and different areas. 17 Phytochemical studies have revealed that Z. jujuba var. inermis contains various constituents, including triterpenoid acids, flavonoids, cerebrosides, amino acids, phenolic acids, polysaccharides, and lignans.…”

Corrective effects of original bioflavonoid complex in the cyclophosphamide-induced immunity disorders

“…Иммуномодулирующие свойства выявлены и у других компонентов КБ. Например, для изофлавонов сои характерна высокая антиоксидантная активность -способность связывать свободные радикалы кислорода, а также противовоспалительные свойства, в частности за счет снижения уровня IL-18 [24]. Эпигаллокатехин-галлат зеленого чая обладает иммуномодулирующими свойствами посредством регуляции PI3K/AKT сигнального пути [14].…”

Nutritional evaluation and transcriptome analyses of short-time germinated seeds in soybean (Glycine max L. Merri.)

“…Although higher nutritional value has been suggested in hypocotyl and root part of soybean seeds 23 , 47 , and large-scale transcriptional analysis has been conducted on axis at early germination phase 48 , the nutrient value and genome-wide gene expression pattern of whole seeds germinated for short-time has not been fully investigated. Considering the importance of entire seeds, especially the cotyledon accounting for the most part of soybean seeds, the objectives of this study were to evaluate the major nutrients in whole seeds germinated for 36 h, and investigate the gene expression pattern dynamically at 0HAG (Hours After Germination), 18HAG, and 36HAG respectively.…”