Isoflavone glucoside genistin, an inhibitor targeting Sortase A and Listeriolysin O, attenuates the virulence of Listeria monocytogenes in vivo and in vitro

Liu, Minda; Lv, Qianghua; Xu, Jingwen; Liu, Baichen; Zhou, Yonglin; Zhang, Siqi; Shen, Xue; Wang, Lin

doi:10.1016/j.bcp.2023.115447

Cited by 6 publications

(2 citation statements)

References 58 publications

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“…Thereafter, 10 µL CCK‐8 detection solution was added to each well and further incubated for 1 h. Finally, the OD value was measured using the microplate detector (BioTek) at OD 450 and the percentage of living cells was calculated. After cells exposed to LPC, LPB or the commonly used positive control 0.2% Triton X‐100 (M. Liu et al, 2023) for 10 h, the cell culture supernatant was collected to detect lactate dehydrogenase (LDH) release using kits in accordance with the manufacturer's instruction (Nanjing Jiancheng Bioengineering Institute).…”

Section: Methodsmentioning

confidence: 99%

Lactobacillus plantarum postbiotics trigger AMPK‐dependent autophagy to suppress Salmonella intracellular infection and NLRP3 inflammasome activation

Wu¹,

Hu²,

Shu³

et al. 2023

Journal Cellular Physiology

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We previously found that Lactobacillus plantarum (LP)‐derived postbiotics protected animals against Salmonella infection, but the molecular mechanism remains obscure. This study clarified the mechanisms from the perspective of autophagy. Intestinal porcine epithelial cells (IPEC‐J2) were pretreated with LP‐derived postbiotics (the culture supernatant, LPC; or heat‐killed bacteria, LPB), and then challenged with Salmonella enterica Typhimurium (ST). Results showed that LP postbiotics markedly triggered autophagy under ST infection, as indicated by the increased LC3 and Beclin1 and the decreased p62 levels. Meanwhile, LP postbiotics (particularly LPC) exhibited a strong capacity of inhibiting ST adhesion, invasion and replication. Pretreatment with the autophagy inhibitor 3‐methyladenine (3‐MA) led to a significant decrease of autophagy and the aggravated infection, indicating the importance of autophagy in LP postbiotics‐mediated Salmonella elimination. LP postbiotics (especially LPB) significantly suppressed ST‐induced inflammation by modulating inflammatory cytokines (the increased interleukin (IL)‐4 and IL‐10, and decreased tumor necrosis factor‐α (TNF), IL‐1β, IL‐6 and IL‐18). Furthermore, LP postbiotics inhibited NOD‐like receptor protein 3 (NLRP3) inflammasome activation, as evidenced by the decreased levels of NLRP3, Caspase‐1 and apoptosis‐associated speck‐like protein containing a caspase recruitment domain (ASC). Deficits in autophagy resulted in an increase of inflammatory response and inflammasome activation. Finally, we found that both LPC and LPB triggered AMP‐activated protein kinase (AMPK) signaling pathway to induce autophagy, and this was further confirmed by AMPK RNA interference. The intracellular infection and NLRP3 inflammasome were aggravated after AMPK knockdown. In summary, LP postbiotics trigger AMPK‐mediated autophagy to suppress Salmonella intracellular infection and NLRP3 inflammasome in IPEC‐J2 cells. Our findings highlight the effectiveness of postbiotics, and provide a new strategy for preventing Salmonella infection.

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Section: Methodsmentioning

confidence: 99%

Lactobacillus plantarum postbiotics trigger AMPK‐dependent autophagy to suppress Salmonella intracellular infection and NLRP3 inflammasome activation

Wu¹,

Hu²,

Shu³

et al. 2023

Journal Cellular Physiology

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“…In addition, the four compounds specific to TM are Genistein-7-O-Glucoside, Salvianolic acid A, Petunidin-3-O-(6″-O-p-Coumaroyl)glucoside and Luteolin-7-O-glucuronide-(2→1)-(2″-Feruloyl)glucuronide. These four compounds, which have a strong antioxidant activity [ 40 , 41 , 42 ], may be the reason why the antioxidant activity of TM is stronger than that of other species.…”

Section: Resultsmentioning

confidence: 99%

Comprehensive Analysis of Secondary Metabolites of Four Medicinal Thyme Species Used in Folk Medicine and Their Antioxidant Activities In Vitro

et al. 2023

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Thyme is a colloquial term for number of aromatic species belonging to the genus Thymus L., known for their expressed biological activities and therefore used worldwide for seasoning and in folk medicine. In the present paper, the content of the total polyphenols (TP), total flavonoids (TF), and antioxidant capacity were assessed in the extracts of four traditionally used thyme species. Moreover, a comprehensive metabolomic study of thyme bioactive compounds was performed, and the obtained data were processed using multivariate statistical tests. The results clearly demonstrated the positive correlation between the content of the TP, TF, and antioxidant activity, and TF was more significant than TP. The findings revealed that four selected thyme species contained 528 secondary metabolites, including 289 flavonoids and 146 phenolic acids. Thymus marschallianus had a higher concentration of active ingredients, which improve its antioxidant capacity. Differentially accumulated metabolites were formed by complex pathways such as flavonoid, flavone, flavonol, isoflavonoid, and anthocyanin biosynthesis. Correlation analysis showed that 59 metabolites (including 28 flavonoids, 18 phenolic acids, and 7 terpenoid compounds) were significantly correlated with obtained values of the antioxidant capacity. The results suggested that selected thyme species exhibit a great diversity in antioxidant-related components, whereas flavonoids may be responsible for the high antioxidant capacity of all studied thyme species. The present study greatly expands our understanding of the complex phytochemical profiles and related applications of selected medicinal plants.

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Harnessing the combined effect of antivirulence agent trans-chalcone with bactericidal curcumin against sortase A enzyme to tackle Gram-positive bacterial infections

Kumari,

Banerjee,

Murty

et al. 2023

Folia Microbiol

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Isoflavone glucoside genistin, an inhibitor targeting Sortase A and Listeriolysin O, attenuates the virulence of Listeria monocytogenes in vivo and in vitro

Cited by 6 publications

References 58 publications

Lactobacillus plantarum postbiotics trigger AMPK‐dependent autophagy to suppress Salmonella intracellular infection and NLRP3 inflammasome activation

Lactobacillus plantarum postbiotics trigger AMPK‐dependent autophagy to suppress Salmonella intracellular infection and NLRP3 inflammasome activation

Comprehensive Analysis of Secondary Metabolites of Four Medicinal Thyme Species Used in Folk Medicine and Their Antioxidant Activities In Vitro

Harnessing the combined effect of antivirulence agent trans-chalcone with bactericidal curcumin against sortase A enzyme to tackle Gram-positive bacterial infections

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