Isoflavone-Free Soy Protein Prepared by Column Chromatography Reduces Plasma Cholesterol in Rats

Fukui, Kensuke; Tachibana, Nobuhiko; Wanezaki, Satoshi; Tsuzaki, Shinichi; Takamatsu, Kiyoharu; Yamamoto, Takashi; Hashimoto, Yukio; Shimoda, Tadahisa

doi:10.1021/jf025642f

Cited by 101 publications

(67 citation statements)

References 23 publications

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“…3,7,8,[22][23][24] In our study the isoflavones were not determined in the b-conglycinin fraction; however, McVeigh et al 25 and Adams et al 26 showed in humans and monkeys that a soy protein diet low in isoflavones produces the same effect on cholesterol levels as a soy protein diet high in isoflavones. These products may be vastly different when comparing studies and=or distinct sources.…”

Section: B-conglycinin In Hypercholesterolemic Ratscontrasting

confidence: 54%

Soy β-Conglycinin (7S Globulin) Reduces Plasma and Liver Cholesterol in Rats Fed Hypercholesterolemic Diet

Ferreira

Silva

Demonte

et al. 2011

Journal of Medicinal Food

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The aim of this study was to examine the comparative hypocholesterolemic effect of soybean 7S fraction in rats fed a high-cholesterol diet. Soybean 7S globulin (b-conglycinin) was administered orally once a day to rats, and the effects were measured after 28 days. Wistar rats were divided into four groups: standard diet (STD) (casein alone), hypercholesterolemic (HC) diet (STD plus 1 g=100 g cholesterol and 0.5 g=100 g cholic acid), HCþ7S 1 diet (HC diet plus 200 mg of 7S=kg of body weight=day), and HCþ7S 2 diet (HC diet plus 300 mg of 7S=kg of body weight=day). Food intake, weight gain, animals' growth, and feeding efficiency ratio were similar among the STD and three HC groups, indicating that these parameters were not affected by treatments. Animals that had received different doses of soybean 7S globulin had lower total cholesterol (TC), triglycerides (TG), and low-density lipoprotein (LDL)=high-density lipoprotein (HDL) ratio in serum and lower levels of hepatic TC and TG than those fed only the HC diet. The atherogenic indexes of HCþ7S 1 and HCþ7S 2 groups were 40% and 55% lower than that of the HC group, respectively. The results showed that the oral daily administration of bconglycinin in the diet to HC rats, at between 1.85% and 2.75% of total ingested protein, promotes the reduction of TC, LDLcholesterol, and TG and an increase in HDL-cholesterol in the plasma, besides a small but significant reduction in cholesterol and TG levels in the liver of the animals as well as a reduced atherogenic index.

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Section: B-conglycinin In Hypercholesterolemic Ratscontrasting

confidence: 54%

Soy β-Conglycinin (7S Globulin) Reduces Plasma and Liver Cholesterol in Rats Fed Hypercholesterolemic Diet

Ferreira

Silva

Demonte

et al. 2011

Journal of Medicinal Food

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“…In recent years, peptides have been identified to possess physiological functions, such as immunomodulatory [20], antimicrobial [21], antihypertensive [22], anticancer [23], antioxidative [24] and cholesterollowering activities [25]. These bioactive peptides are mostly derived from milk, wheat, soybean, egg and fish proteins by enzymatic hydrolysis or fermentation [26].…”

Section: Introductionmentioning

confidence: 99%

Dipeptidyl Peptidase-IV Inhibitory Activity of Peptides in Porcine Skin Gelatin Hydrolysates

Hsu¹,

Tung²,

Huang³

et al. 2013

Bioactive Food Peptides in Health and Disease

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“…Dietary soy isoflavones, including genistein, have received great attention as anti-atherogenic foods due to their lipid-improving effects [15][16][17][18][19], but in vivo and in vitro findings regarding their total and/or LDL cholesterol-lowering effects have been inconsistent [20][21][22][23][24][25]. Some studies report that dietary supplementation with genistein, a strong bioactive soy isoflavone, lowers total and LDL-cholesterol levels [23,24], while others describe no effect of soy isoflavones on the concentration of LDL-cholesterol even when vascular function is improved [25].…”

Section: Introductionmentioning

confidence: 99%

JNK-Mediated SREBP-2 Processing by Genistein up-regulates LDLR Expression in HepG2 Cells

Lee

Han²,

Kim³

2014

J Nutr Food Sci

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Genistein has been implicated for its anti-atherogenic effects. We investigated the molecular mechanisms behind the impact of genistein on expression of LDLR, the receptor for LDL-cholesterol, and related signaling pathways in HepG2 cells. Genistein increased mRNA and protein levels of LDLR in a time-dependent manner. In order to find out the effects of genistein on the transcriptional levels, a luciferase reporter construct containing LDLR promoter (pLDLR-luc) was constructed and examined for its response to genistein. Genistein increased the reporter activity but failed to increase transcriptional activity when sterol-regulatory element (SRE) in the LDLR promoter was deleted. Genistein increased nuclear translocation of SREBP-2 and DNA binding activity of SREBP-2 to LDLR promoter by chromatin immunoprecipitation assay (CHIP). Pre-treatment of 4-(2-aminoethyl) benzenesulfonyl fluoride (AEBSF), serine protease inhibitor, prevented the effects of genistein while brefeldin A causing the fusion of the endoplasmic reticulum (ER) and the Golgi apparatus did not, suggesting that genistein may have an effect on SREBP-2 trafficking from the ER to the Golgi apparatus. Insig-1 protein levels were not changed by genistein. Among mitogen-activated protein kinases (MAPK), genistein phosphorylated JNK but not p38 and ERK signals. JNK inhibitor (SP600126) abolished genistein-stimulated levels of LDLR and nuclear SREBP-2. To minimize the effects of c-Jun, a transcription factor activated by JNK signals, a truncated LDLR luciferase construct that contained SRE but lacked the c-jun putative binding site was constructed. Genistein still was able to boost the transcriptional activity of the truncated LDLR construct. All the genistein effects were abolished by the addition of cholesterol. In conclusion, genistein has the anti-atherogenic effects by activating JNK signals and SREBP-2 processing, which is followed by up-regulation of LDLR. However, the beneficial effects of genistein could be affected by the amount of cellular cholesterols.

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Isoflavone-Free Soy Protein Prepared by Column Chromatography Reduces Plasma Cholesterol in Rats

Cited by 101 publications

References 23 publications

Soy β-Conglycinin (7S Globulin) Reduces Plasma and Liver Cholesterol in Rats Fed Hypercholesterolemic Diet

Soy β-Conglycinin (7S Globulin) Reduces Plasma and Liver Cholesterol in Rats Fed Hypercholesterolemic Diet

Dipeptidyl Peptidase-IV Inhibitory Activity of Peptides in Porcine Skin Gelatin Hydrolysates

JNK-Mediated SREBP-2 Processing by Genistein up-regulates LDLR Expression in HepG2 Cells

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