Isoflavone Content and Composition in Soybean

Tepavčević, Vesna; Cvejić, Jelena; Poša, Mihalj; Popović, Jovan

doi:10.5772/14736

Cited by 4 publications

(2 citation statements)

References 47 publications

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“…), a representative leguminous plant, contains a high abundance of isoflavones. However, significant differences have been noted in the total isoflavone content of soybean depending on the country or region of cultivation [20]. Wild soybean (Glycine soja Siebold & Zucc.…”

Section: Introductionmentioning

confidence: 99%

Production of Daidzein and Genistein from Seed and Root Extracts of Korean Wild Soybean (Glycine soja) by Thermostable β-Galactosidase from Thermoproteus uzoniensis

Shin

Kang

et al. 2022

Applied Sciences

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Isoflavone glycosides are commonly biotransformed into isoflavone aglycones due to the superior biological activities of the latter. Wild soybeans contain a higher isoflavone content than domesticated soybeans due to their high level of genetic diversity. In this study, we cloned and characterized a thermostable β-galactosidase from the extreme thermophile Thermoproteus uzoniensis for potential application in isoflavone conversion in Korean wild soybeans. The purified recombinant enzyme exhibited a maximum specific activity of 1103 μmol/min/mg at pH 5.0 and 90 °C with a half-life of 46 h and exists as a homodimer of 113 kDa. The enzyme exhibited the highest activity for p-nitrophenyl (pNP)-β-D-galactopyranoside among aryl glycosides and it hydrolyzed isoflavone glycosides in the order genistin > daidzin > ononin > glycitin. The enzyme completely hydrolyzed 2.77 mM daidzin and 3.85 mM genistin in the seed extract of wild soybean after 80 and 70 min with productivities of 1.86 and 3.30 mM/h, respectively, and 9.89 mM daidzin and 1.67 mM genistin in the root extract after 180 and 30 min, with the highest productivities of 3.30 and 3.36 mM/h, respectively, compared to other glycosidases. Our results will contribute to the industrial production of isoflavone aglycone using wild soybean and this is the first report on the enzymatic production of isoflavone aglycones from isoflavone glycosides in wild soybeans.

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Section: Introductionmentioning

confidence: 99%

Production of Daidzein and Genistein from Seed and Root Extracts of Korean Wild Soybean (Glycine soja) by Thermostable β-Galactosidase from Thermoproteus uzoniensis

Shin

Kang

et al. 2022

Applied Sciences

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“…Generally, the flavonoids most present in soybean are isoflavones (daidzen, glycitein, genistein and their glucosides), which are extensively studied for their estrogen-like properties [9]. Content and composition of isoflavones in soybeans is largely dependent on plant genotype, as well as the environmental conditions [10,11]. During incompatible reactions, daidzein (dihydroxylated isoflavone), immediate precursor of the glyceollins, as well as genistein (trihydroxylated isoflavone) are accumulated in soybean tissues [8].…”

Section: Introductionmentioning

confidence: 99%

Changes in L-phenylalanine ammonia-lyase activity and isoflavone phytoalexins accumulation in soybean seedlings infected with Sclerotinia sclerotiorum

et al. 2013

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Soybean [Glycine max (L.) Merr.] cultivars (Meli, Alisa, Sava and 1511/99) were grown up to V1 phase (first trifoliate and one node above unifoliate) and then inoculated with Sclerotinia sclerotiorum (Lib.) de Bary under controlled conditions. Changes in L-phenylalanine ammonia-lyase (PAL) activity and isoflavone phytoalexins were recorded 12, 24, 48 and 72 h after the inoculation. Results showed an increase in PAL activity in all four examined soybean cultivars 48 h after the inoculation, being the highest in Alisa (2-fold higher). Different contents of total daidzein, genistein, glycitein and coumestrol were detected in all samples. Alisa and Sava increased their total isoflavone content (33.9% and 6.2% higher than control, respectively) as well as 1511/99, although 48 h after the inoculation its content decreased significantly. Meli exhibited the highest rate of coumestrol biosynthesis (72 h after the inoculation) and PAL activity (48 h after the inoculation). All investigated cultivars are invariably susceptible to this pathogen. Recorded changes could point to possible differences in mechanisms of tolerance among them.

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Effect of activated carbon on greenhouse gas emissions, seed yield, soil chemical properties and isoflavone content of soybean genotypes with varying nodulation capacities under sandy soil conditions

et al. 2020

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Isoflavone Content and Composition in Soybean

Cited by 4 publications

References 47 publications

Production of Daidzein and Genistein from Seed and Root Extracts of Korean Wild Soybean (Glycine soja) by Thermostable β-Galactosidase from Thermoproteus uzoniensis

Production of Daidzein and Genistein from Seed and Root Extracts of Korean Wild Soybean (Glycine soja) by Thermostable β-Galactosidase from Thermoproteus uzoniensis

Changes in L-phenylalanine ammonia-lyase activity and isoflavone phytoalexins accumulation in soybean seedlings infected with Sclerotinia sclerotiorum

Effect of activated carbon on greenhouse gas emissions, seed yield, soil chemical properties and isoflavone content of soybean genotypes with varying nodulation capacities under sandy soil conditions

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