Isoflavin‐β modifies muscle oxidative stress and prevents a thyrotoxicosis‐induced loss of muscle mass in rats

Marinello, Poliana Camila; Bernardes, Sara Santos; Guarnier, Flávia Alessandra; Silva, Thamara N X Da; Borges, Fernando H.; Lopes, Natália Medeiros Dias; Simão, Andréa Name Colado; Armani, André; Cecchini, Rúbens; Cecchini, Alessandra Lourenço

doi:10.1002/mus.25546

Cited by 8 publications

(5 citation statements)

References 39 publications

(74 reference statements)

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“…Indeed, astaxanthin—a powerful antioxidant—was reported to attenuate immobilization-induced increased collagen deposition via modulation of oxidative stress, most notably via altered SOD-1 and TGF- β signaling in rats [24]. Similarly, the antioxidant flavone mixture isoflavin- β was reported to attenuate toxin-induced loss of gastrocnemius muscle mass by preventing the oxidative modification of proteins [30].…”

Section: Discussionmentioning

confidence: 99%

Redox Status and Muscle Pathology in Rheumatoid Arthritis: Insights from Various Rat Hindlimb Muscles

Oyenihi

Ollewagen

Myburgh

et al. 2019

Oxidative Medicine and Cellular Longevity

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Due to atrophy, muscle weakness is a common occurrence in rheumatoid arthritis (RA). The majority of human studies are conducted on the vastus lateralis muscle—a muscle with mixed fiber type—but little comparative data between multiple muscles in either rodent or human models are available. The current study therefore assessed both muscle ultrastructure and selected redox indicators across various muscles in a model of collagen-induced rheumatoid arthritis in female Sprague-Dawley rats. Only three muscles, the gastrocnemius, extensor digitorum longus (EDL), and soleus, had lower muscle mass (38%, 27%, and 25% loss of muscle mass, respectively; all at least P<0.01), while the vastus lateralis muscle mass was increased by 35% (P<0.01) in RA animals when compared to non-RA controls. However, all four muscles exhibited signs of deterioration indicative of rheumatoid cachexia. Cross-sectional area was similarly reduced in gastrocnemius, EDL, and soleus (60%, 58%, and 64%, respectively; all P<0.001), but vastus lateralis (22% smaller, P<0.05) was less affected, while collagen deposition was significantly increased in muscles. This pathology was associated with significant increases in tissue levels of reactive oxygen species (ROS) in all muscles except the vastus lateralis, while only the gastrocnemius had significantly increased levels of lipid peroxidation (TBARS) and antioxidant activity (FRAP). Current data illustrates the differential responses of different skeletal muscles of the hindlimb to a chronic inflammatory challenge both in terms of redox changes and resistance to cachexia.

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Section: Discussionmentioning

confidence: 99%

Redox Status and Muscle Pathology in Rheumatoid Arthritis: Insights from Various Rat Hindlimb Muscles

Oyenihi

Ollewagen

Myburgh

et al. 2019

Oxidative Medicine and Cellular Longevity

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“…Cytochrome c, which is trapped in the cristae junction compartment, is released upon damage to the mitochondrial ultrastructure and activates the caspase pathway that executes apoptosis 31 . Excessive or prolonged energy demands and oxidative stress can lead to ROSmediated mitochondrial damage and dysfunction 22 . Short duration exogenous levothyroxine administration (for 3 days) increased the expression of uncoupling proteins and decreased the mitochondrial e ciency of skeletal muscles in male subjects 37 .…”

Section: Discussionmentioning

confidence: 99%

“…We observed that the mTOR pathway was inhibited by thyroid hormones, and the catabolic action of thyroid hormone excess enhanced the expression of MuRF1, but lean mass did not decrease compared to the ovariectomy group. The demand for energy in the skeletal muscles is provided for by the increased hepatic glucose production rate and metabolic activity induced by hyperthyroidism 22 . As in previous studies 25,28 , creatinine kinase (CK), a marker of muscle damage, was not elevated in hyperthyroidism.…”

Section: Discussionmentioning

confidence: 99%

“…Similar to a high fat diet, estrogen de ciency due to ovariectomy can lead to skeletal muscle dysfunction due to increased oxidative stress in muscles and mitochondrial dysfunction 21 . Some previous preclinical studies have investigated the effects of thyroid hormone on skeletal muscles 3,22 , but studies investigating hormonal changes in a female model remain insu cient. Therefore, the present study was performed to investigate changes in the histology and molecular mechanisms of skeletal muscles in levothyroxine-induced hyperthyroidism in ovariectomy animal model.…”

Section: Introductionmentioning

confidence: 99%

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The Effect of TSH-Suppressive Dose of Levothyroxine On Skeletal Muscle In Ovariectomized Rats

Kim

Lee

et al. 2021

Preprint

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Hyperthyroidism is often observed in postmenopausal women due to conditions such as thyroiditis and toxic nodular goiter, Grave’s disease or thyroid stimulating hormone suppressive therapy for treating differentiated thyroid carcinoma (DTC). However, the effect of such hormonal changes on skeletal muscles in females remain unclear. Therefore, this study aimed to observe the effects of hyperthyroidism on the skeletal muscle of ovariectomized rats. We randomly divided female Sprague-Dawley rats into sham-operated (Sham), ovariectomized (OVX), and levothyroxine-treated ovariectomized groups (OVX+LT4). Levothyroxine was administered intraperitoneally at 0.3 mg/kg, daily for six weeks. Protein synthesis was increased after ovariectomy whereas protein synthesis was suppressed and protein degradation was increased in response to levothyroxine treatment. However, there was no difference in lean mass between the two groups. Collagen I levels were similar between the Sham and OVX groups, but were significantly decreased in the OVX+LT4 group. The mRNA levels of matrix metalloproteinase (MMP) ‐2 and ‐9 were similar between the Sham and OVX groups but were upregulated in the OVX+LT4 group. After ovariectomy, mitochondrial biogenesis and dynamics were changed; these changes were exacerbated in hyperthyroidism. Our findings indicate that in postmenopausal rats with hyperthyroidism, the progression of muscle weakness occurs through impaired regulation of signaling pathways related to extracellular matrix homeostasis, protein turnover, and mitochondrial quality.

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“…Whereafter, Aoyama et al found that dietary genistein supplementation attenuates denervation-induced muscle atrophy in male C57BL/6J mice [115]. Daily intraperitoneal injections of isoflavin-β, a mixture of isoflavones, prevents thyrotoxicosis-induced muscle mass loss in Wistar rats through increasing muscle total antioxidant capacity and decreasing mitochondrial cytochrome oxidase activity [116].…”

Section: Flavonoidsmentioning

confidence: 99%

Nutraceuticals in the Prevention and Treatment of the Muscle Atrophy

Wang

Liu²,

Quan

et al. 2021

Nutrients

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Imbalance of protein homeostasis, with excessive protein degradation compared with protein synthesis, leads to the development of muscle atrophy resulting in a decrease in muscle mass and consequent muscle weakness and disability. Potential triggers of muscle atrophy include inflammation, malnutrition, aging, cancer, and an unhealthy lifestyle such as sedentariness and high fat diet. Nutraceuticals with preventive and therapeutic effects against muscle atrophy have recently received increasing attention since they are potentially more suitable for long-term use. The implementation of nutraceutical intervention might aid in the development and design of precision medicine strategies to reduce the burden of muscle atrophy. In this review, we will summarize the current knowledge on the importance of nutraceuticals in the prevention of skeletal muscle mass loss and recovery of muscle function. We also highlight the cellular and molecular mechanisms of these nutraceuticals and their possible pharmacological use, which is of great importance for the prevention and treatment of muscle atrophy.

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Isoflavin‐β modifies muscle oxidative stress and prevents a thyrotoxicosis‐induced loss of muscle mass in rats

Abstract: Iso-β decreased oxidative modification of proteins, which is known to exert a major role during proteolysis induction and is present in thyrotoxic myopathy, highlighting the potential action of Iso-β in this complication of the disease. Muscle Nerve 56: 975-981, 2017.

Cited by 8 publications

References 39 publications

Redox Status and Muscle Pathology in Rheumatoid Arthritis: Insights from Various Rat Hindlimb Muscles

Redox Status and Muscle Pathology in Rheumatoid Arthritis: Insights from Various Rat Hindlimb Muscles

The Effect of TSH-Suppressive Dose of Levothyroxine On Skeletal Muscle In Ovariectomized Rats

Nutraceuticals in the Prevention and Treatment of the Muscle Atrophy

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