Isoenzymmuster der Phosphoglucomutase der menschlichen Spermien (Sp.-PGM1)

Renninger, W.; Sina, D.

doi:10.1007/bf00297643

Cited by 14 publications

(1 citation statement)

References 6 publications

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“…Only the PGM 1 1 t y p e shows an additional weak isoenzyme migrating slightly faster t h a n isoenzyme "d" of the PGM 1 2 --1 type. This spot is identical with an isoenzyme described b y Renninger and Sina (1970) and designated "d'" b y B r i n k m a n n and Koops (1971) of the PGM 1 1 t y p e of h u m a n sperm cell lysates. So far, we have detected this isoenzyme (d') of red cell lysates in all PGM 1 1 types.…”

Section: The Pgm Systemmentioning

confidence: 94%

Comments on the determination of isoenzyme polymorphism (ADA, AK, 6-PGD, PGM) by cellulose acetate electrophoresis

Sonneborn

1972

Hum Genet

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Summary. The paper highlights certain peculiarities of isoenzyme polymorphism as determined by cellulose acetate electrophoresis, as compared to other supporting media at different pH levels and concentrations of the buffer.Zusammen/assung. Es werden Besonderheiten bei der Bestimmung der Isoenzymmuster mit der Celluloseacetatfolien-Elektrophorese in bezug auf andere TrEgermedien als auch in bezug auf pH-Wert und Konzentration der verwendeten Puffer beschrieben.Methods for the determination of isoenzyme polymorpbism by cellulose acetate electrophoresis have been previously described for adenosine deaminase (ADA), adenylate kinase (AK) and phosphoglucomutase (PGM) Renninger, 1970a, b, 1971;Sonneborn, 1971).This report describes a method for the determination of 6-phosphoglueonate dehydrogenase (6-PGD) isoenzymes. Iv also discussed certain peculiarities of the isoenzyme systems in relation to other supporting media and the pattern and number of spots, as well as the manner in which they are influenced by pH level and buffer concentration. Materials and MethodsFor electrophoretic studies we used the CAF electrophoretie film system and the BiotestSerum-Institut cellulose acetate film (7.5 X 15 cm). :For all investigations we used red cell lysates obtained by freezing for 12 hrs at --20°C.Buffer for staining solution: Tris/ItC1 buffer 0.03 M, pH 7.9 (containing 0.01 M MgC12 • 6 H20). (Properties of the buffer used for electrophoresis are given separately for each isoenzyme system.) Electrophoresis: 90 min at room temperature and 23 V/cm. The isoenzymes were stained by the sandwich method.Preparation of the agar solution: Dissolve 300 mg agar in 20 ml staining buffer in a boiling water bath and allow solution to cool to 50°C.Heat 15 ml staining buffer to 50°C and add the following reagents 5 to 10 min before the end of electrophoresis::For PG!VI staining: 10 mg NADP, 70 mg glucose-l-phosphate, 0.5 mg glucose-l,6-diphosphate, 5 mg PMS, 7 mg ~TT and 20 izl glucose-6-phosphate dehydrogenase (purity grade IX, 1 mg/ml, Boehringer).For AK staining: 150 mg glucose, 30 mg ADP, 10 mg NADP, 5 mg PMS, 5 mg MTT, 20 ~l glucose-6-phosphate dehydrogenase and 20 izl hexokinase (2 mg/ml, Boehringer). 4 t-Iumangenetik, Bd. 17

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Section: The Pgm Systemmentioning

confidence: 94%

Comments on the determination of isoenzyme polymorphism (ADA, AK, 6-PGD, PGM) by cellulose acetate electrophoresis

Sonneborn

1972

Hum Genet

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Phosphoglucomutase (PGM) and 6-phosphogluconate dehydrogenase (PGD) isozymes in human sperm cells

Brinkmann

1971

Hum Genet

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Investigations on the polymorphism of sperm diaphorase in man

et al. 1977

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The polymorphism of sperm diaphorase (SD) was investigated in 141 unrelated persons from Hessen, Germany, by high voltage thin-layer agarose gel electrophoresis (Age) and thin-layer isoelectric focusing on polyacrylamide gel (Pagif). In addition to the three known common phenotypes SD 1, 2-1, and 2, two further phenotypes with the preliminary designation SD 3-1 and SD 3-2 were discovered. This polymorphism can thus be explained in terms of three alleles, SD1, SD2, and SD3 segregating at an autosomal locus. The allele frequencies calculated from the five different phenotypes SD 1, 2, 2-1, 3-1, and 3-2 are: SD1 = 0.7553, SD2 = 0.2234, and SD3 = 0.0213. As we also found SD activity in female reproductive tract tissues (ovaries, oviducts, uterus), the term 'gonadal diaphorase' (GD) appears to be applicable.

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Isoenzymmuster der Phosphoglucomutase der menschlichen Spermien (Sp.-PGM1)

Cited by 14 publications

References 6 publications

Comments on the determination of isoenzyme polymorphism (ADA, AK, 6-PGD, PGM) by cellulose acetate electrophoresis

Comments on the determination of isoenzyme polymorphism (ADA, AK, 6-PGD, PGM) by cellulose acetate electrophoresis

Phosphoglucomutase (PGM) and 6-phosphogluconate dehydrogenase (PGD) isozymes in human sperm cells

Investigations on the polymorphism of sperm diaphorase in man

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